mouse monoclonal igg1 antihuman contactin  (R&D Systems)


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    R&D Systems mouse monoclonal igg1 antihuman contactin
    Mouse Monoclonal Igg1 Antihuman Contactin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat polyclonal anti tag1  (R&D Systems)


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    R&D Systems goat polyclonal anti tag1
    Goat Polyclonal Anti Tag1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti tag1 europium donor fluorescence detection  (Tecan Systems)

     
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    Tecan Systems anti tag1 europium donor fluorescence detection
    Anti Tag1 Europium Donor Fluorescence Detection, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat polyclonal anti tag1  (R&D Systems)


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    R&D Systems goat polyclonal anti tag1
    Goat Polyclonal Anti Tag1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal igg1 antihuman contactin  (R&D Systems)


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    R&D Systems mouse monoclonal igg1 antihuman contactin
    Mouse Monoclonal Igg1 Antihuman Contactin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat anti tag1 polyclonal antibody  (R&D Systems)


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    R&D Systems goat anti tag1 polyclonal antibody
    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Goat Anti Tag1 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types"

    Article Title: A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types

    Journal: Science Advances

    doi: 10.1126/sciadv.adk2149

    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Figure Legend Snippet: ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].

    Techniques Used: Labeling, Mutagenesis, Comparison


    Structured Review

    Jackson Immuno tag1
    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Tag1, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types"

    Article Title: A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types

    Journal: Science Advances

    doi: 10.1126/sciadv.adk2149

    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Figure Legend Snippet: ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].

    Techniques Used: Labeling, Mutagenesis, Comparison

    anti tag 1  (R&D Systems)


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    R&D Systems anti tag 1
    Anti Tag 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti tag1  (Danaher Inc)


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    Danaher Inc anti tag1
    Anti Tag1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Developmental Studies Hybridoma Bank anti tag1
    Anti Tag1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems mouse monoclonal igg1 antihuman contactin
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    R&D Systems goat anti tag1 polyclonal antibody
    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
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    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Tag1, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Anti Tag 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
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    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
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    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to <t>Tag1</t> and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].
    Anti Tag1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].

    Journal: Science Advances

    Article Title: A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types

    doi: 10.1126/sciadv.adk2149

    Figure Lengend Snippet: ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].

    Article Snippet: The primary antibodies used were rabbit anti-Barhl1 polyclonal antibody (Atlas Antibodies; HPA004809, Sigma-Aldrich; 1:500), goat anti-Robo3 polyclonal antibody (R&D Systems, AF3076; 1:200), goat anti-Tag1 polyclonal antibody (R&D Systems, AF4439; 1:500), mouse anti–NF-160kD monoclonal antibody (clone RMO-270; Thermo Fisher Scientific, 13-0700; 1:500), rat anti-L1CAM monoclonal antibody (clone 324; Merck Millipore, MAB5272; 1:400), goat anti-DCC polyclonal antibody (Santa Cruz Biotechnology, sc-6535; 1:200), rabbit anti-FoxP2 polyclonal antibody (Abcam, ab16046; 1:1000), mouse anti-Brn3a monoclonal antibody (clone 5A3.2; Merck Millipore, MAB1585; 1;200), rabbit anti-Lim1/Lhx1 antibody (Abcam, ab229474; 1:250), rabbit anti-Lhx2 (Invitrogen, PA5-78287; 1:250), rat anti-HA (Roche; clone3F10; 1:100), guinea pig anti-Isl1 antibody (a gift from Y. Tanabe, Kyoto University, Japan), and chick anti-GFP polyclonal antibody (Abcam, ab13970; 1:1500).

    Techniques: Labeling, Mutagenesis, Comparison

    ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].

    Journal: Science Advances

    Article Title: A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types

    doi: 10.1126/sciadv.adk2149

    Figure Lengend Snippet: ( A ) The schematic shows the PN formation in an E16.5 hindbrain. Red dashed lines mark the rostral (r) and caudal (c) span of the sections shown on the right. PN is visualized by Barhl1 IHC. PN neurons form a nucleus adjacent to the ventral midline (arrows) ( n = 3) in the control but were laterally positioned (arrowheads) indicating failure to approach the ventral midline in the mutants ( n = 3). ( B to D ) Coronal sections from the spinal cord (B), hindbrain (C), and midbrain (D) at E11.5 were subjected to Tag1 and NF double IHC with the Tag1 labeling the commissural axons and the NF signals depicting the general axonal patterns. The DAPI counterstain indicates the overall cytoarchitecture. Comparisons were made between the control genotype ( Nhlh1 +/+ Nhlh2 +/m ) ( n = 3) and the double mutant ( Nhlh1 m/m Nhlh2 m/m ) ( n = 3). The Tag1 and NF merged images in the bottom panel are high-magnification images of the ventral commissure regions. The double mutant showed a complete lack of ventral commissures (hollow arrows), in comparison to the control (filled arrows). Scale bars, 200 μm [(A) to (D)].

    Article Snippet: The secondary antibodies used were Cy3-donkey anti-rabbit immunoglobulin G (IgG) (Jackson ImmunoResearch, 1:300) for Barhl1, FoxP2, Lhx1, and Lhx2 antibodies; Cy3-donkey anti-goat IgG (Jackson ImmunoResearch, 1:300) for Robo3, Tag1, and DCC antibodies; Cy3-donkey anti-mouse IgG (Jackson ImmunoResearch, 1:300) for NF and Brn3a antibodies; Cy3-donkey anti-rat IgG (Jackson ImmunoResearch, 1:300) for an L1CAM antibody; Alexa Fluor 488–donkey anti-chick IgY (Jackson ImmunoResearch, 1:400) for a GFP antibody; and Alexa Fluor 647–donkey anti-guinea pig-IgG (Jackson ImmunoResearch 1:400) for Isl1 antibody.

    Techniques: Labeling, Mutagenesis, Comparison