anti rabbit nr2a  (Alomone Labs)


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    Alomone Labs anti rabbit nr2a
    Assessment of <t>NR2A</t> and NR2B expression in the PrV by Western blot analysis. (A) NR2A and NR2B protein levels at P5 and P14 in the developing and denervated PrV. (B) NR2A and NR2B expression on the normal side of the PrV at different ages. The intensity
    Anti Rabbit Nr2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit nr2a/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit nr2a - by Bioz Stars, 2022-12
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    1) Product Images from "NMDA RECEPTOR SUBUNIT COMPOSITION IN THE RAT TRIGEMINAL PRINCIPAL NUCLEUS REMAINS CONSTANT DURING POSTNATAL DEVELOPMENT AND FOLLOWING NEONATAL DENERVATION"

    Article Title: NMDA RECEPTOR SUBUNIT COMPOSITION IN THE RAT TRIGEMINAL PRINCIPAL NUCLEUS REMAINS CONSTANT DURING POSTNATAL DEVELOPMENT AND FOLLOWING NEONATAL DENERVATION

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2011.01.023

    Assessment of NR2A and NR2B expression in the PrV by Western blot analysis. (A) NR2A and NR2B protein levels at P5 and P14 in the developing and denervated PrV. (B) NR2A and NR2B expression on the normal side of the PrV at different ages. The intensity
    Figure Legend Snippet: Assessment of NR2A and NR2B expression in the PrV by Western blot analysis. (A) NR2A and NR2B protein levels at P5 and P14 in the developing and denervated PrV. (B) NR2A and NR2B expression on the normal side of the PrV at different ages. The intensity

    Techniques Used: Expressing, Western Blot

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    Alomone Labs agc 002
    Changes in AMPAR density and subunit composition in the VTA of sexually satiated rats and involvement of eCBs in their induction. Western blot analysis showing changes in <t>GluA1</t> (A) and GluA2 AMPAR subunit (B) densities in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of males that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA followed by Tukey test, *** P
    Agc 002, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    agc 002 - by Bioz Stars, 2022-12
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    Changes in AMPAR density and subunit composition in the VTA of sexually satiated rats and involvement of eCBs in their induction. Western blot analysis showing changes in GluA1 (A) and GluA2 AMPAR subunit (B) densities in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of males that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA followed by Tukey test, *** P

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Endocannabinoids Released in the Ventral Tegmental Area During Copulation to Satiety Modulate Changes in Glutamate Receptors Associated With Synaptic Plasticity Processes

    doi: 10.3389/fnsyn.2021.701290

    Figure Lengend Snippet: Changes in AMPAR density and subunit composition in the VTA of sexually satiated rats and involvement of eCBs in their induction. Western blot analysis showing changes in GluA1 (A) and GluA2 AMPAR subunit (B) densities in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of males that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA followed by Tukey test, *** P

    Article Snippet: The following primary antibodies were used: rabbit anti-CB1R, mouse anti-β-actin, goat anti-β-Arrestin2 (β-A2), mouse anti-GluN2B and mouse anti-GLUA2 (Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-GluN2A, rabbit anti-GluN1and rabbit anti-GluA1 (Alomone Labs, Jerusalem, Israel); rabbit anti-CB1R phospho S316 (pCB1R) (Abcam, Cambridge, UK), rabbit anti-ERK 1/2 phospho Thr202/Tyr204 (pERK 1/2) (Cell Signaling Technology, Danvers, MA, USA) and mouse anti-VGlut2 (Merck-Millipore, Burlington, MA, USA).

    Techniques: Western Blot

    Changes in VTA NMDAR subunit composition produced by copulation to satiety in male rats and involvement of eCBs in their induction. Western blot analysis showing changes in GluN2A (A) and GluN2B (B) NMDAR subunit expression in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of rats that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA followed by Tukey test, *** P

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Endocannabinoids Released in the Ventral Tegmental Area During Copulation to Satiety Modulate Changes in Glutamate Receptors Associated With Synaptic Plasticity Processes

    doi: 10.3389/fnsyn.2021.701290

    Figure Lengend Snippet: Changes in VTA NMDAR subunit composition produced by copulation to satiety in male rats and involvement of eCBs in their induction. Western blot analysis showing changes in GluN2A (A) and GluN2B (B) NMDAR subunit expression in the VTA of control sexually experienced unmated rats (Control), males that ejaculated once (1 Ejac) or copulated to satiety (Sexually satiated) 24 h earlier, and of rats that copulated to satiety in the presence of the CB1R antagonist AM251 (Satiated + AM251). Differences among untreated groups with different sexual conditions were determined by means of a one-way ANOVA followed by Tukey test, *** P

    Article Snippet: The following primary antibodies were used: rabbit anti-CB1R, mouse anti-β-actin, goat anti-β-Arrestin2 (β-A2), mouse anti-GluN2B and mouse anti-GLUA2 (Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-GluN2A, rabbit anti-GluN1and rabbit anti-GluA1 (Alomone Labs, Jerusalem, Israel); rabbit anti-CB1R phospho S316 (pCB1R) (Abcam, Cambridge, UK), rabbit anti-ERK 1/2 phospho Thr202/Tyr204 (pERK 1/2) (Cell Signaling Technology, Danvers, MA, USA) and mouse anti-VGlut2 (Merck-Millipore, Burlington, MA, USA).

    Techniques: Produced, Western Blot, Expressing

    Full-length NMDA receptors are expressed by cultured HPASMCs. Cultured HPASMCs were lysed and immunoprecipitated with GluN1- or GluN2- subunit specific antibodies or control non-immune IgG, and the pellets were analyzed by Western blot. Immunopositive bands containing full-length GluN1 ( A ) and GluN2 (2A–D) ( B – E ) protein were detected in immune pellets from lysates of PASMCs but not in the IgG control conditions studied in parallel. Cropped images are shown for conciseness. Full-length blots are represented in Supplementary Fig. S9 . Results are representative of three separate experiments.

    Journal: Scientific Reports

    Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-021-87667-0

    Figure Lengend Snippet: Full-length NMDA receptors are expressed by cultured HPASMCs. Cultured HPASMCs were lysed and immunoprecipitated with GluN1- or GluN2- subunit specific antibodies or control non-immune IgG, and the pellets were analyzed by Western blot. Immunopositive bands containing full-length GluN1 ( A ) and GluN2 (2A–D) ( B – E ) protein were detected in immune pellets from lysates of PASMCs but not in the IgG control conditions studied in parallel. Cropped images are shown for conciseness. Full-length blots are represented in Supplementary Fig. S9 . Results are representative of three separate experiments.

    Article Snippet: After 3 washes in TBS, sections were permeabilized and pre-blocked followed by primary antibody incubation at the following concentrations: platelet endothelial cell adhesion molecule (PECAM-1) (ab28364, Abcam, Cambridge, MA) (1/150), GluN1 (556308, BD Pharmingen, San Jose, CA) (1/1000) (this reference contains characterization of the antibody against GluN1), GluN2A (AGC-002, Alomone, Jerusalem, Israel) (1/20,000), GluN2B (AGC-003, Alomone, Jerusalem, Israel) (1/3000), GluN2C (ab105146, Abcam, Cambridge, MA) (1/30,000) or GluN2D (ab186816, Abcam, Cambridge, MA) (1/10,000).

    Techniques: Cell Culture, Immunoprecipitation, Western Blot

    NMDA receptor expression by HPASMCs in vivo . Human lung tissue containing pulmonary artery was stained with NMDA receptor subunit specific antibodies. PECAM-1 was used as a marker for vascular endothelial cells (EC). Antibody against PECAM-1 revealed immunoreactivity within the interior surface of pulmonary artery. Immunoreactive staining for both GluN1 ( B ) and four GluN2 subunits (2A–D) ( C – F ) was observed in pulmonary artery smooth muscle cells (SMC). Control experiments with primary antibody omitted showed no immunoreactivity in either pulmonary artery endothelial cells or PASMCs ( G ). Scale bar = 50 μM.

    Journal: Scientific Reports

    Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-021-87667-0

    Figure Lengend Snippet: NMDA receptor expression by HPASMCs in vivo . Human lung tissue containing pulmonary artery was stained with NMDA receptor subunit specific antibodies. PECAM-1 was used as a marker for vascular endothelial cells (EC). Antibody against PECAM-1 revealed immunoreactivity within the interior surface of pulmonary artery. Immunoreactive staining for both GluN1 ( B ) and four GluN2 subunits (2A–D) ( C – F ) was observed in pulmonary artery smooth muscle cells (SMC). Control experiments with primary antibody omitted showed no immunoreactivity in either pulmonary artery endothelial cells or PASMCs ( G ). Scale bar = 50 μM.

    Article Snippet: After 3 washes in TBS, sections were permeabilized and pre-blocked followed by primary antibody incubation at the following concentrations: platelet endothelial cell adhesion molecule (PECAM-1) (ab28364, Abcam, Cambridge, MA) (1/150), GluN1 (556308, BD Pharmingen, San Jose, CA) (1/1000) (this reference contains characterization of the antibody against GluN1), GluN2A (AGC-002, Alomone, Jerusalem, Israel) (1/20,000), GluN2B (AGC-003, Alomone, Jerusalem, Israel) (1/3000), GluN2C (ab105146, Abcam, Cambridge, MA) (1/30,000) or GluN2D (ab186816, Abcam, Cambridge, MA) (1/10,000).

    Techniques: Expressing, In Vivo, Staining, Marker

    NMDA receptor expression in cultured HPASMCs. Immunofluorescence staining was performed on permeabilized HPASMCs. Network-like immunoreactive staining was observed for both GluN1 and all four GluN2 (2A–D) subunits. Control experiments with primary antibody omitted showed no immunoreactivity. Results are representative images taken from at least three separate experiments. Scale bar = 50 μM.

    Journal: Scientific Reports

    Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-021-87667-0

    Figure Lengend Snippet: NMDA receptor expression in cultured HPASMCs. Immunofluorescence staining was performed on permeabilized HPASMCs. Network-like immunoreactive staining was observed for both GluN1 and all four GluN2 (2A–D) subunits. Control experiments with primary antibody omitted showed no immunoreactivity. Results are representative images taken from at least three separate experiments. Scale bar = 50 μM.

    Article Snippet: After 3 washes in TBS, sections were permeabilized and pre-blocked followed by primary antibody incubation at the following concentrations: platelet endothelial cell adhesion molecule (PECAM-1) (ab28364, Abcam, Cambridge, MA) (1/150), GluN1 (556308, BD Pharmingen, San Jose, CA) (1/1000) (this reference contains characterization of the antibody against GluN1), GluN2A (AGC-002, Alomone, Jerusalem, Israel) (1/20,000), GluN2B (AGC-003, Alomone, Jerusalem, Israel) (1/3000), GluN2C (ab105146, Abcam, Cambridge, MA) (1/30,000) or GluN2D (ab186816, Abcam, Cambridge, MA) (1/10,000).

    Techniques: Expressing, Cell Culture, Immunofluorescence, Staining

    Functional NMDA receptors are localized on the surface of HPASMCs. Immunofluorescence staining was performed on non-permeabilized HPASMCs. Both GluN1 and GluN2 (2B and 2D) subunits were detected on the surface of HPASMCs ( A , B ). GluN1 co-localized with GluN2B ( A ) and GluN2D ( B ) on the surface of HPASMCs, respectively. Results are representative images taken from at least three separate experiments. Scale bar = 100 µM. ( C , D ) are representative scatter plots of each fluorescent pixel from confocal images with GluN1 green fluorescent intensity along the x -axis and GluN2 red fluorescent intensity along the y -axis. The shaded area in the upper right quadrant represents colocalized pixels above background with the associated Pearson correlation coefficient indicated for all colocalized pixels. ( E ) Representative traces for cultured HPASMCs in response to 100 μM NMDA and 10 μM glycine treatment. Cells were clamped at − 50 mV and whole cell recordings were performed. ( F ) NMDA and glycine treatment evoked an inward whole-cell current of 10.9 ± 1.7 pA (Mean ± SE, **p

    Journal: Scientific Reports

    Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-021-87667-0

    Figure Lengend Snippet: Functional NMDA receptors are localized on the surface of HPASMCs. Immunofluorescence staining was performed on non-permeabilized HPASMCs. Both GluN1 and GluN2 (2B and 2D) subunits were detected on the surface of HPASMCs ( A , B ). GluN1 co-localized with GluN2B ( A ) and GluN2D ( B ) on the surface of HPASMCs, respectively. Results are representative images taken from at least three separate experiments. Scale bar = 100 µM. ( C , D ) are representative scatter plots of each fluorescent pixel from confocal images with GluN1 green fluorescent intensity along the x -axis and GluN2 red fluorescent intensity along the y -axis. The shaded area in the upper right quadrant represents colocalized pixels above background with the associated Pearson correlation coefficient indicated for all colocalized pixels. ( E ) Representative traces for cultured HPASMCs in response to 100 μM NMDA and 10 μM glycine treatment. Cells were clamped at − 50 mV and whole cell recordings were performed. ( F ) NMDA and glycine treatment evoked an inward whole-cell current of 10.9 ± 1.7 pA (Mean ± SE, **p

    Article Snippet: After 3 washes in TBS, sections were permeabilized and pre-blocked followed by primary antibody incubation at the following concentrations: platelet endothelial cell adhesion molecule (PECAM-1) (ab28364, Abcam, Cambridge, MA) (1/150), GluN1 (556308, BD Pharmingen, San Jose, CA) (1/1000) (this reference contains characterization of the antibody against GluN1), GluN2A (AGC-002, Alomone, Jerusalem, Israel) (1/20,000), GluN2B (AGC-003, Alomone, Jerusalem, Israel) (1/3000), GluN2C (ab105146, Abcam, Cambridge, MA) (1/30,000) or GluN2D (ab186816, Abcam, Cambridge, MA) (1/10,000).

    Techniques: Functional Assay, Immunofluorescence, Staining, Cell Culture

    NMDA receptor mRNA expression in human pulmonary artery. RT-PCR was performed using RNA isolated from human pulmonary artery. The presence of mRNAs for GluN1 and all GluN2 subunits (2A–D) was detected in human pulmonary artery. Human brain RNA was used as a positive control.

    Journal: Scientific Reports

    Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-021-87667-0

    Figure Lengend Snippet: NMDA receptor mRNA expression in human pulmonary artery. RT-PCR was performed using RNA isolated from human pulmonary artery. The presence of mRNAs for GluN1 and all GluN2 subunits (2A–D) was detected in human pulmonary artery. Human brain RNA was used as a positive control.

    Article Snippet: After 3 washes in TBS, sections were permeabilized and pre-blocked followed by primary antibody incubation at the following concentrations: platelet endothelial cell adhesion molecule (PECAM-1) (ab28364, Abcam, Cambridge, MA) (1/150), GluN1 (556308, BD Pharmingen, San Jose, CA) (1/1000) (this reference contains characterization of the antibody against GluN1), GluN2A (AGC-002, Alomone, Jerusalem, Israel) (1/20,000), GluN2B (AGC-003, Alomone, Jerusalem, Israel) (1/3000), GluN2C (ab105146, Abcam, Cambridge, MA) (1/30,000) or GluN2D (ab186816, Abcam, Cambridge, MA) (1/10,000).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Positive Control