anti mouse igg2a antibodies  (Millipore)


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    Name:
    Monoclonal Anti Rat IgG2a antibody
    Description:
    IgGs are glycoprotein antibodies that modulate several immune responses Rat IgGs have four subclasses namely IgG1 IgG2a IgG2b and IgG2c Rat IgGs against target proteins are often used as primary antibodies in various research applications Thus secondary anti rat IgG is a useful tool for the analysis of target proteins Monoclonal Anti Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a The antibody localizes the denatured reduced heavy chain molecule of rat IgG2a γ2a The product recognizes rat IgG2a derived from normal serum or myeloma proteins but does not bind to other rat immunoglobulins The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine cat chicken dog goat horse human mouse pig rabbit and sheep
    Catalog Number:
    r0761
    Price:
    None
    Applications:
    Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and slot blot immunoassay.
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    Structured Review

    Millipore anti mouse igg2a antibodies
    IgG1 and <t>IgG2a</t> subclasses of antibodies elicited after transcutaneous boost with CRM 197 given with or without adjuvant or subcutaneous boost with adsorbed DTxd vaccine. Data are presented as individual anti-CRM 197 or anti-DTxd IgG1 (A) and anti-CRM 197
    IgGs are glycoprotein antibodies that modulate several immune responses Rat IgGs have four subclasses namely IgG1 IgG2a IgG2b and IgG2c Rat IgGs against target proteins are often used as primary antibodies in various research applications Thus secondary anti rat IgG is a useful tool for the analysis of target proteins Monoclonal Anti Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a The antibody localizes the denatured reduced heavy chain molecule of rat IgG2a γ2a The product recognizes rat IgG2a derived from normal serum or myeloma proteins but does not bind to other rat immunoglobulins The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine cat chicken dog goat horse human mouse pig rabbit and sheep
    https://www.bioz.com/result/anti mouse igg2a antibodies/product/Millipore
    Average 95 stars, based on 6075 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg2a antibodies - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Transcutaneous Immunization with Cross-Reacting Material CRM197 of Diphtheria Toxin Boosts Functional Antibody Levels in Mice Primed Parenterally with Adsorbed Diphtheria Toxoid Vaccine "

    Article Title: Transcutaneous Immunization with Cross-Reacting Material CRM197 of Diphtheria Toxin Boosts Functional Antibody Levels in Mice Primed Parenterally with Adsorbed Diphtheria Toxoid Vaccine

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00797-07

    IgG1 and IgG2a subclasses of antibodies elicited after transcutaneous boost with CRM 197 given with or without adjuvant or subcutaneous boost with adsorbed DTxd vaccine. Data are presented as individual anti-CRM 197 or anti-DTxd IgG1 (A) and anti-CRM 197
    Figure Legend Snippet: IgG1 and IgG2a subclasses of antibodies elicited after transcutaneous boost with CRM 197 given with or without adjuvant or subcutaneous boost with adsorbed DTxd vaccine. Data are presented as individual anti-CRM 197 or anti-DTxd IgG1 (A) and anti-CRM 197

    Techniques Used:

    2) Product Images from "Human Cytomegalovirus Inhibits Erythropoietin Production"

    Article Title: Human Cytomegalovirus Inhibits Erythropoietin Production

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2013101125

    Kidney tissue biopsy specimens from patients with CKD are positive for hCMV, and blood hCMV IgG levels inversely correlate with red blood cell count. (A) Biopsy specimens from CKD were stained for hCMV IE proteins; arrows highlight examples of positive
    Figure Legend Snippet: Kidney tissue biopsy specimens from patients with CKD are positive for hCMV, and blood hCMV IgG levels inversely correlate with red blood cell count. (A) Biopsy specimens from CKD were stained for hCMV IE proteins; arrows highlight examples of positive

    Techniques Used: Cell Counting, Staining

    3) Product Images from "Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control"

    Article Title: Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004000

    CD48 undergoes proteolytic degradation during MCMV infection. (A) MEFs and peritoneal macrophages were infected at an moi of 0.025 or 0.1, respectively with wt MCMV and MCMVΔm154. At the indicated days after infection the amount of extracellular (MEFs) or cell-associated (macrophages) infectious virus present in the cultures was determined. Each data point represents the average and standard deviation of three separate cultures. (B) Peritoneal macrophages were mock-infected or infected with wt MCMV at an moi of 10 for 72 h. Whole-cell RNA was harvested, treated with DNase and reverse-transcribed using oligo(dT). PCRs were performed using primer sets specific for murine CD48, m154, and β-actin. The amplified products were separated on agarose gels and visualized with ethidium bromide staining. As shown, specific PCR-amplified products were not detected in control reactions in which the reverse transcriptase (RT) was not added during the reverse transcription reaction. (C) Peritoneal macrophages were mock-infected or infected with wt MCMV at an moi of 10. At the indicated time points after infection, cell lysates were prepared, and subjected to Western blot analysis as indicated in Figure 4B using an anti-mouse CD48 mAb or the anti-m154 mAb followed by anti-Armenian hamster HRP or by anti-mouse IgG HRP, respectively. A mAb anti-β-actin followed by anti-rabbit IgG HRP was used as an internal control. (D) Peritoneal macrophages were mock-infected (mock) or infected with wt MCMV at an moi of 10 in the absence (-) or presence of MG-132 or leupeptin. Cell lysates were prepared at 72 hpi and subjected to Western blot analysis as indicated in C. (E) Peritoneal macrophages were mock-infected or infected with wt MCMV at an moi of 10 for 72 h. When indicated, cultures were treated with MG-132 or leupeptin. Cells were fixed, permeabilized, and stained with anti-CD48-Alexa Fluor 488, and anti-m154 mAb followed by an anti-mouse IgG Alexa fluor 555, and analyzed as indicated in Figure 4C . Shown are representative cells from cultures stained for m154 (panels a, e, i, and m), for CD48 (panels b, f, j, and n), nuclei stained with the DAPI reagent (panels c, g, k, and o), and overlaid images (panels d, h, l, and p). Images were captured at 40× magnification.
    Figure Legend Snippet: CD48 undergoes proteolytic degradation during MCMV infection. (A) MEFs and peritoneal macrophages were infected at an moi of 0.025 or 0.1, respectively with wt MCMV and MCMVΔm154. At the indicated days after infection the amount of extracellular (MEFs) or cell-associated (macrophages) infectious virus present in the cultures was determined. Each data point represents the average and standard deviation of three separate cultures. (B) Peritoneal macrophages were mock-infected or infected with wt MCMV at an moi of 10 for 72 h. Whole-cell RNA was harvested, treated with DNase and reverse-transcribed using oligo(dT). PCRs were performed using primer sets specific for murine CD48, m154, and β-actin. The amplified products were separated on agarose gels and visualized with ethidium bromide staining. As shown, specific PCR-amplified products were not detected in control reactions in which the reverse transcriptase (RT) was not added during the reverse transcription reaction. (C) Peritoneal macrophages were mock-infected or infected with wt MCMV at an moi of 10. At the indicated time points after infection, cell lysates were prepared, and subjected to Western blot analysis as indicated in Figure 4B using an anti-mouse CD48 mAb or the anti-m154 mAb followed by anti-Armenian hamster HRP or by anti-mouse IgG HRP, respectively. A mAb anti-β-actin followed by anti-rabbit IgG HRP was used as an internal control. (D) Peritoneal macrophages were mock-infected (mock) or infected with wt MCMV at an moi of 10 in the absence (-) or presence of MG-132 or leupeptin. Cell lysates were prepared at 72 hpi and subjected to Western blot analysis as indicated in C. (E) Peritoneal macrophages were mock-infected or infected with wt MCMV at an moi of 10 for 72 h. When indicated, cultures were treated with MG-132 or leupeptin. Cells were fixed, permeabilized, and stained with anti-CD48-Alexa Fluor 488, and anti-m154 mAb followed by an anti-mouse IgG Alexa fluor 555, and analyzed as indicated in Figure 4C . Shown are representative cells from cultures stained for m154 (panels a, e, i, and m), for CD48 (panels b, f, j, and n), nuclei stained with the DAPI reagent (panels c, g, k, and o), and overlaid images (panels d, h, l, and p). Images were captured at 40× magnification.

    Techniques Used: Infection, Standard Deviation, Amplification, Staining, Polymerase Chain Reaction, Western Blot

    Analysis of MCMV m154. (A) The deduced amino acid sequence of m154 is shown, with the predicted leader peptide in yellow and the transmembrane region in blue. The putative N-linked glycosylation site (underlined) and O-glycosylation sites (bold) are indicated. (B) Peritoneal macrophages were mock-infected (mock) or infected with wt MCMV at an moi of 10 in the absence (lane -) or presence of cycloheximide and actinomycin D (lane CHX), or phosphonoacetic acid (lane PPA). Cell lysates were prepared at 72 hpi, except for the cycloheximide treated samples that were collected 16 hpi, separated under reducing conditions by SDS-PAGE (10%), and transferred to a nitrocellulose membrane. The blot was proved with the anti-m154 mAb or anti-MCMV IE1 (Croma 101) mAb followed by anti-mouse IgG HRP. A mAb anti-β-actin followed by an anti-rabbit IgG HRP was used as an internal control. (C) Peritoneal macrophages, either mock-infected or infected with wt MCMV at an moi of 10 for 72 h, were fixed, permeabilized, and stained with anti-m154 mAb followed by an anti-mouse IgG Alexa fluor 555. Nuclei were stained with the DAPI reagent. The cells were examined under a microscope at 405 nm (DAPI, panels a and d) and at 555–565 nm (Alexa-555, panels b and e). The overlaid images are shown in panels c and f. (×10 magnification). (D) Peritoneal macrophages, either mock-infected (mock) or infected with wt MCMV at an moi of 10, were surface-labeled with biotin. Cell lysates were prepared at 72 hpi and subjected to immunoprecipitation with anti-m154 mAb, followed by SDS-PAGE (10%) separation and Western blot analysis as indicated in B, using streptavidin-POD conjugate. Molecular weights in kilodaltons are indicated. (E) Flow cytometry analysis of 300.19 cells stably transfected with empty pDisplay vector expressing HA (left panel) or the construct HA-m154 (right panel) and stained after 24 h with biotin anti-HA mAb (black line) or isotype control (dashed line) followed by streptavidin-PE. (F) 300.19 cells non-transfected (shaded histogram) or stably transfected with the construct HA-m154 (black line histogram) were analyzed by flow cytometry for surface expression of CD48 as indicated in Figure 1 . The corresponding isotype was used as a negative control (dashed line histogram).
    Figure Legend Snippet: Analysis of MCMV m154. (A) The deduced amino acid sequence of m154 is shown, with the predicted leader peptide in yellow and the transmembrane region in blue. The putative N-linked glycosylation site (underlined) and O-glycosylation sites (bold) are indicated. (B) Peritoneal macrophages were mock-infected (mock) or infected with wt MCMV at an moi of 10 in the absence (lane -) or presence of cycloheximide and actinomycin D (lane CHX), or phosphonoacetic acid (lane PPA). Cell lysates were prepared at 72 hpi, except for the cycloheximide treated samples that were collected 16 hpi, separated under reducing conditions by SDS-PAGE (10%), and transferred to a nitrocellulose membrane. The blot was proved with the anti-m154 mAb or anti-MCMV IE1 (Croma 101) mAb followed by anti-mouse IgG HRP. A mAb anti-β-actin followed by an anti-rabbit IgG HRP was used as an internal control. (C) Peritoneal macrophages, either mock-infected or infected with wt MCMV at an moi of 10 for 72 h, were fixed, permeabilized, and stained with anti-m154 mAb followed by an anti-mouse IgG Alexa fluor 555. Nuclei were stained with the DAPI reagent. The cells were examined under a microscope at 405 nm (DAPI, panels a and d) and at 555–565 nm (Alexa-555, panels b and e). The overlaid images are shown in panels c and f. (×10 magnification). (D) Peritoneal macrophages, either mock-infected (mock) or infected with wt MCMV at an moi of 10, were surface-labeled with biotin. Cell lysates were prepared at 72 hpi and subjected to immunoprecipitation with anti-m154 mAb, followed by SDS-PAGE (10%) separation and Western blot analysis as indicated in B, using streptavidin-POD conjugate. Molecular weights in kilodaltons are indicated. (E) Flow cytometry analysis of 300.19 cells stably transfected with empty pDisplay vector expressing HA (left panel) or the construct HA-m154 (right panel) and stained after 24 h with biotin anti-HA mAb (black line) or isotype control (dashed line) followed by streptavidin-PE. (F) 300.19 cells non-transfected (shaded histogram) or stably transfected with the construct HA-m154 (black line histogram) were analyzed by flow cytometry for surface expression of CD48 as indicated in Figure 1 . The corresponding isotype was used as a negative control (dashed line histogram).

    Techniques Used: Sequencing, Infection, SDS Page, Staining, Microscopy, Labeling, Immunoprecipitation, Western Blot, Flow Cytometry, Cytometry, Stable Transfection, Transfection, Plasmid Preparation, Expressing, Construct, Negative Control

    4) Product Images from "Expression of Human Herpesvirus-6 Antigens in Benign and Malignant Lymphoproliferative Diseases"

    Article Title: Expression of Human Herpesvirus-6 Antigens in Benign and Malignant Lymphoproliferative Diseases

    Journal: The American Journal of Pathology

    doi:

    A: AILD; negative staining with an isotype-matched control mouse mAb against human IgG1. Immunoperoxidase method; magnification, ×300. B: Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease); negative staining with isotype-matched control mouse mAb against human IgG2b. Immunoperoxidase method; ×200.
    Figure Legend Snippet: A: AILD; negative staining with an isotype-matched control mouse mAb against human IgG1. Immunoperoxidase method; magnification, ×300. B: Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease); negative staining with isotype-matched control mouse mAb against human IgG2b. Immunoperoxidase method; ×200.

    Techniques Used: Negative Staining

    Related Articles

    Modification:

    Article Title: Induction of tropomyosin during hepatic stellate cell activation and the progression of liver fibrosis
    Article Snippet: .. Mouse monoclonal IgG2a antibody against α-SMA, mouse monoclonal IgG1 antibody against tropomyosin, Dulbecco’s modified Eagle’s medium (DMEM), TAA, and fetal bovine serum (FBS) were purchased from Sigma Chemical Co. (Saint Louis, MO, USA). .. Rabbit polyclonal IgG antibodies against human platelet-derived growth factor receptor-β (PDGFR-β) that reacts with rat PDGFR-β were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Incubation:

    Article Title: Alternative Inactivated Poliovirus Vaccines Adjuvanted with Quillaja brasiliensis or Quil-A Saponins Are Equally Effective in Inducing Specific Immune Responses
    Article Snippet: .. After 1 h at 37°C, plates were washed three times with PBS-T and incubated with adequate dilutions of peroxidase conjugated anti-mouse IgG (Sigma), anti-mouse IgG1 (Sigma) or anti-mouse IgG2a (Sigma) for 1 h at 37°C. .. After washing, 100 µl of OPD (ortho -phenylenediamine) Sigma with 0.03% of H2 O2 were added to each well.

    Article Title: Human Cytomegalovirus Inhibits Erythropoietin Production
    Article Snippet: .. Mouse monoclonal IgG2a antibodies specific for CMV IE antigen (clone 8B1.2; Chemicon International) or a negative control antibody against keratin 20 (United States Biologic) were added and sections incubated overnight at 4°C. .. For visualization, we used a horseradish peroxidase detection system (Super Sensitive Link Label Detection System; BioGenex) and positive cells were visualized with chromogen diaminobenzidine (Innovex Biosciences).

    Negative Control:

    Article Title: PI3Kγ integrates cAMP and Akt signalling of the μ-opioid receptor
    Article Snippet: .. Sections 5 μm thick of paraformaldehyde-fixed and paraffin-embedded DRG-tissues from wild-type and PI3Kγ knockout mice were labelled with anti-PI3Kγ monoclonal antibody (641) or mouse anti-IgG2a (Sigma) as isotype negative control. ..

    Article Title: Human Cytomegalovirus Inhibits Erythropoietin Production
    Article Snippet: .. Mouse monoclonal IgG2a antibodies specific for CMV IE antigen (clone 8B1.2; Chemicon International) or a negative control antibody against keratin 20 (United States Biologic) were added and sections incubated overnight at 4°C. .. For visualization, we used a horseradish peroxidase detection system (Super Sensitive Link Label Detection System; BioGenex) and positive cells were visualized with chromogen diaminobenzidine (Innovex Biosciences).

    Knock-Out:

    Article Title: PI3Kγ integrates cAMP and Akt signalling of the μ-opioid receptor
    Article Snippet: .. Sections 5 μm thick of paraformaldehyde-fixed and paraffin-embedded DRG-tissues from wild-type and PI3Kγ knockout mice were labelled with anti-PI3Kγ monoclonal antibody (641) or mouse anti-IgG2a (Sigma) as isotype negative control. ..

    Mouse Assay:

    Article Title: PI3Kγ integrates cAMP and Akt signalling of the μ-opioid receptor
    Article Snippet: .. Sections 5 μm thick of paraformaldehyde-fixed and paraffin-embedded DRG-tissues from wild-type and PI3Kγ knockout mice were labelled with anti-PI3Kγ monoclonal antibody (641) or mouse anti-IgG2a (Sigma) as isotype negative control. ..

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  • 93
    Millipore anti rabbit igg gamma chain specific biotin antibody mouse monoclonal
    Anti Rabbit Igg Gamma Chain Specific Biotin Antibody Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg gamma chain specific biotin antibody mouse monoclonal/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg gamma chain specific biotin antibody mouse monoclonal - by Bioz Stars, 2020-08
    93/100 stars
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