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Jackson Immuno cy3 conjugated streptavidin
Effects of estradiol on translocation of estrogen receptor alpha (ERα) to mitochondria Human osteoblast-like U2OS cells were exposed to 10 nM of estradiol for 1, 6, 12, and 24 h. Distribution of the ERα protein in human osteoblasts was immunodetected using an antibody with <t>Cy3-conjugated</t> <t>streptavidin</t> ( A , top panel). Mitochondria of human osteoblasts were stained with 3,3′-dihexyloxacarbocyanine (DiOC6), a positively charged dye (middle panel). Merged signals indicated that the ERα protein had been translocated into mitochondria (bottom panels). These fluorescent signals were quantified and statistically analyzed (B) . Each value represents the mean ± SEM for n = 6. The symbol * indicates that the value significantly differed from the respective control group, p
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Images

1) Product Images from "Estrogen/ERα signaling axis participates in osteoblast maturation via upregulating chromosomal and mitochondrial complex gene expressions"

Article Title: Estrogen/ERα signaling axis participates in osteoblast maturation via upregulating chromosomal and mitochondrial complex gene expressions

Journal: Oncotarget

doi: 10.18632/oncotarget.23453

Effects of estradiol on translocation of estrogen receptor alpha (ERα) to mitochondria Human osteoblast-like U2OS cells were exposed to 10 nM of estradiol for 1, 6, 12, and 24 h. Distribution of the ERα protein in human osteoblasts was immunodetected using an antibody with Cy3-conjugated streptavidin ( A , top panel). Mitochondria of human osteoblasts were stained with 3,3′-dihexyloxacarbocyanine (DiOC6), a positively charged dye (middle panel). Merged signals indicated that the ERα protein had been translocated into mitochondria (bottom panels). These fluorescent signals were quantified and statistically analyzed (B) . Each value represents the mean ± SEM for n = 6. The symbol * indicates that the value significantly differed from the respective control group, p
Figure Legend Snippet: Effects of estradiol on translocation of estrogen receptor alpha (ERα) to mitochondria Human osteoblast-like U2OS cells were exposed to 10 nM of estradiol for 1, 6, 12, and 24 h. Distribution of the ERα protein in human osteoblasts was immunodetected using an antibody with Cy3-conjugated streptavidin ( A , top panel). Mitochondria of human osteoblasts were stained with 3,3′-dihexyloxacarbocyanine (DiOC6), a positively charged dye (middle panel). Merged signals indicated that the ERα protein had been translocated into mitochondria (bottom panels). These fluorescent signals were quantified and statistically analyzed (B) . Each value represents the mean ± SEM for n = 6. The symbol * indicates that the value significantly differed from the respective control group, p

Techniques Used: Translocation Assay, Staining

Effects of estradiol on translocation of estrogen receptor alpha (ERα) to nuclei Human osteoblast-like U2OS cells were exposed to 10 nM of estradiol for 1, 6, 12, and 24 h. Distribution of the ERα protein in human osteoblasts was immunodetected using an antibody with Cy3-conjugated streptavidin ( A , top panel). Cellular nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (middle panel). The merged signals indicated that the ERα protein had been translocated into nuclei (bottom panel). These merged fluorescent signals were quantified and statistically analyzed (B) . Each value represents the mean ± SEM for n = 6. The symbol * indicates that the value significantly differed from the respective control group, p
Figure Legend Snippet: Effects of estradiol on translocation of estrogen receptor alpha (ERα) to nuclei Human osteoblast-like U2OS cells were exposed to 10 nM of estradiol for 1, 6, 12, and 24 h. Distribution of the ERα protein in human osteoblasts was immunodetected using an antibody with Cy3-conjugated streptavidin ( A , top panel). Cellular nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (middle panel). The merged signals indicated that the ERα protein had been translocated into nuclei (bottom panel). These merged fluorescent signals were quantified and statistically analyzed (B) . Each value represents the mean ± SEM for n = 6. The symbol * indicates that the value significantly differed from the respective control group, p

Techniques Used: Translocation Assay, Staining

2) Product Images from "UDP-Glucuronosyltransferase 1a Enzymes Are Present and Active in the Mouse Blastocyst"

Article Title: UDP-Glucuronosyltransferase 1a Enzymes Are Present and Active in the Mouse Blastocyst

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.114.059766

Confocal immunofluorescence analysis of Ugt expression and localization in blastocysts. (A) Exemplary images of blastocysts stained with pan-specific antibodies against Ugt1a and Ugt2b, with DAPI staining to show the cell nuclei. Strong Ugt1a signal is
Figure Legend Snippet: Confocal immunofluorescence analysis of Ugt expression and localization in blastocysts. (A) Exemplary images of blastocysts stained with pan-specific antibodies against Ugt1a and Ugt2b, with DAPI staining to show the cell nuclei. Strong Ugt1a signal is

Techniques Used: Immunofluorescence, Expressing, Staining

3) Product Images from "Neuromyelitis optica study model based on chronic infusion of autoantibodies in rat cerebrospinal fluid"

Article Title: Neuromyelitis optica study model based on chronic infusion of autoantibodies in rat cerebrospinal fluid

Journal: Journal of Neuroinflammation

doi: 10.1186/s12974-016-0577-8

Axonal damage and loss in the spinal cord and optic nerve of the NMO-rat. a Axon injury detected in the NMO-rat compared to the Control-rat (rats infused with IgG AQP4+ 2 and IgG Control 2, D7) using neurofilament immunostaining: reduced number of axons detected as NF-M-positive spots in the white matter (WM); fragmentation and reduced axon thickness in the gray matter (GM). b Classification (10–20 to 100–140 μm 2 , ImageJ) and quantification (mean by field) of NF-M-positive spots in the spinal cord of the NMO-rats ( n = 6) compared to the Control-rats ( n = 6): loss of axons with 60–140 μm 2 sections in the NMO-rats (in cart: evaluation of the total axon number, p = 0.03). c Co-detection of myelin alteration (MBP in green ) and axonal loss (neurofilament NF-M subtype in red ) in the spinal cord of the NMO-rat compared to the Control-rat. d Increased expression of the NF-H phosphorylated form, a marker of axon injury, detected by Western blot (pNF-H/NF-H ratio; p = 0.04). e Axon fragmentation and loss in the optic nerve of the NMO-rats compared to the Control-rat detected by NF-M immunostaining. Scale bars = 20 μm
Figure Legend Snippet: Axonal damage and loss in the spinal cord and optic nerve of the NMO-rat. a Axon injury detected in the NMO-rat compared to the Control-rat (rats infused with IgG AQP4+ 2 and IgG Control 2, D7) using neurofilament immunostaining: reduced number of axons detected as NF-M-positive spots in the white matter (WM); fragmentation and reduced axon thickness in the gray matter (GM). b Classification (10–20 to 100–140 μm 2 , ImageJ) and quantification (mean by field) of NF-M-positive spots in the spinal cord of the NMO-rats ( n = 6) compared to the Control-rats ( n = 6): loss of axons with 60–140 μm 2 sections in the NMO-rats (in cart: evaluation of the total axon number, p = 0.03). c Co-detection of myelin alteration (MBP in green ) and axonal loss (neurofilament NF-M subtype in red ) in the spinal cord of the NMO-rat compared to the Control-rat. d Increased expression of the NF-H phosphorylated form, a marker of axon injury, detected by Western blot (pNF-H/NF-H ratio; p = 0.04). e Axon fragmentation and loss in the optic nerve of the NMO-rats compared to the Control-rat detected by NF-M immunostaining. Scale bars = 20 μm

Techniques Used: Immunostaining, Expressing, Marker, Western Blot

4) Product Images from "Combinatorial synthesis and screening of cancer cell-specific nanomedicines targeted via phage fusion proteins"

Article Title: Combinatorial synthesis and screening of cancer cell-specific nanomedicines targeted via phage fusion proteins

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2015.00628

Purification and characterization of DMPGTVLP-modified Lipodox . Size exclusion chromatography of modified Lipodox on a Superose 6 column (30 × 1 cm), 2.5 mL/fractions 4–11 of the elution profile are shown. Western blot of recovered fractions probed with a polyclonal anti-fd rabbit primary IgG, followed by a biotinylated goat anti-rabbit secondary IgG and detected with NeutrAvadin-HRP and Pico West luminol substrate. Physicochemical characterization of recovered fractions for size distribution (d.nm), zeta potential (mV), doxorubicin recovery (μg), and percent doxorubicin recovery (%).
Figure Legend Snippet: Purification and characterization of DMPGTVLP-modified Lipodox . Size exclusion chromatography of modified Lipodox on a Superose 6 column (30 × 1 cm), 2.5 mL/fractions 4–11 of the elution profile are shown. Western blot of recovered fractions probed with a polyclonal anti-fd rabbit primary IgG, followed by a biotinylated goat anti-rabbit secondary IgG and detected with NeutrAvadin-HRP and Pico West luminol substrate. Physicochemical characterization of recovered fractions for size distribution (d.nm), zeta potential (mV), doxorubicin recovery (μg), and percent doxorubicin recovery (%).

Techniques Used: Purification, Modification, Size-exclusion Chromatography, Western Blot

5) Product Images from "UDP-Glucuronosyltransferase 1a Enzymes Are Present and Active in the Mouse Blastocyst"

Article Title: UDP-Glucuronosyltransferase 1a Enzymes Are Present and Active in the Mouse Blastocyst

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.114.059766

Detection of Ugt proteins in mouse blastocysts. (A) Proteins of the Ugt1a family were present as detected by a UGT1A/Ugt1a pan-specific antibody (55 kDa). (B) Murine Ugt1a1 was not detected (52 kDa). (C) Murine Ugt1a3 was not detected (37 kDa). (D) Murine
Figure Legend Snippet: Detection of Ugt proteins in mouse blastocysts. (A) Proteins of the Ugt1a family were present as detected by a UGT1A/Ugt1a pan-specific antibody (55 kDa). (B) Murine Ugt1a1 was not detected (52 kDa). (C) Murine Ugt1a3 was not detected (37 kDa). (D) Murine

Techniques Used:

Confocal immunofluorescence analysis of Ugt expression and localization in blastocysts. (A) Exemplary images of blastocysts stained with pan-specific antibodies against Ugt1a and Ugt2b, with DAPI staining to show the cell nuclei. Strong Ugt1a signal is
Figure Legend Snippet: Confocal immunofluorescence analysis of Ugt expression and localization in blastocysts. (A) Exemplary images of blastocysts stained with pan-specific antibodies against Ugt1a and Ugt2b, with DAPI staining to show the cell nuclei. Strong Ugt1a signal is

Techniques Used: Immunofluorescence, Expressing, Staining

6) Product Images from "Flavivirus Infection Activates the XBP1 Pathway of the Unfolded Protein Response To Cope with Endoplasmic Reticulum Stress ▿"

Article Title: Flavivirus Infection Activates the XBP1 Pathway of the Unfolded Protein Response To Cope with Endoplasmic Reticulum Stress ▿

Journal: Journal of Virology

doi: 10.1128/JVI.00879-06

Flavivirus induces XBP1 mRNA splicing in infected cultured neuronal cells and mouse brains. (A) The analysis scheme of XBP1 mRNA splicing. The relative locations of the 26-nt intron, the PstI recognition site, and the PCR-amplified region are shown. The sizes of PCR-amplified fragments from spliced and unspliced XBP1 with or without PstI cleavage are also listed. (B) XBP1 mRNA splicing and induction of downstream genes. N18, a mouse neuroblastoma cell line, was mock infected (lanes 1 to 4), treated with tunicamycin (1 μg/ml; lanes 5 to 8), and infected with JEV (lanes 9 to 12) or DEN-2 (lanes 13 to 16). The MOI was 3. Cells were harvested at 6, 12, 24, or 36 h after treatment for RT-PCR analysis using primer sets specific for the genes shown on the left. The PCR product of XBP1 was further analyzed by PstI digestion. DNA molecules were separated by 2 or 1.5% agarose gel electrophoresis, stained with ethidium bromide, and photographed with a Photo-Print photodocumentation system (Vilber Lourmat). (C) The induction ( n -fold) of selected UPR downstream genes was quantified by real-time RT-PCR as described in Materials and Methods. (D) Decrease in full-length ATF6 (p90ATF6) in flavivirus-infected cells. N18 cells were treated with 2 mM dithiothreitol (DTT) for 3 h or infected with JEV or DEN-2 (MOI, 5) for 20 h before the cell lysates were harvested (62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol) for immunoblotting with anti-ATF6 (IMGENEX) and anti-actin antibodies. (E) The protein expression of XBP1s in N18 cells was analyzed by immunoblotting at several time points after infection with JEV or DEN-2 (MOI, 5) as indicated. The nuclear extracts were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with the antibody indicated on the right. (F) The XBP1 mRNA status in brain lysates of C57/B6 mice intracranially inoculated with JEV (1 × 10 4 PFU/mouse) was analyzed by RT-PCR and PstI digestion. The level of JEV replication was also detected by RT-PCR of the NS3 region of the JEV genome. PBS, mice sham inoculated with PBS serving as a negative control. p.i., postinfection.
Figure Legend Snippet: Flavivirus induces XBP1 mRNA splicing in infected cultured neuronal cells and mouse brains. (A) The analysis scheme of XBP1 mRNA splicing. The relative locations of the 26-nt intron, the PstI recognition site, and the PCR-amplified region are shown. The sizes of PCR-amplified fragments from spliced and unspliced XBP1 with or without PstI cleavage are also listed. (B) XBP1 mRNA splicing and induction of downstream genes. N18, a mouse neuroblastoma cell line, was mock infected (lanes 1 to 4), treated with tunicamycin (1 μg/ml; lanes 5 to 8), and infected with JEV (lanes 9 to 12) or DEN-2 (lanes 13 to 16). The MOI was 3. Cells were harvested at 6, 12, 24, or 36 h after treatment for RT-PCR analysis using primer sets specific for the genes shown on the left. The PCR product of XBP1 was further analyzed by PstI digestion. DNA molecules were separated by 2 or 1.5% agarose gel electrophoresis, stained with ethidium bromide, and photographed with a Photo-Print photodocumentation system (Vilber Lourmat). (C) The induction ( n -fold) of selected UPR downstream genes was quantified by real-time RT-PCR as described in Materials and Methods. (D) Decrease in full-length ATF6 (p90ATF6) in flavivirus-infected cells. N18 cells were treated with 2 mM dithiothreitol (DTT) for 3 h or infected with JEV or DEN-2 (MOI, 5) for 20 h before the cell lysates were harvested (62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol) for immunoblotting with anti-ATF6 (IMGENEX) and anti-actin antibodies. (E) The protein expression of XBP1s in N18 cells was analyzed by immunoblotting at several time points after infection with JEV or DEN-2 (MOI, 5) as indicated. The nuclear extracts were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with the antibody indicated on the right. (F) The XBP1 mRNA status in brain lysates of C57/B6 mice intracranially inoculated with JEV (1 × 10 4 PFU/mouse) was analyzed by RT-PCR and PstI digestion. The level of JEV replication was also detected by RT-PCR of the NS3 region of the JEV genome. PBS, mice sham inoculated with PBS serving as a negative control. p.i., postinfection.

Techniques Used: Infection, Cell Culture, Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Quantitative RT-PCR, Expressing, Polyacrylamide Gel Electrophoresis, Mouse Assay, Negative Control

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TUNEL Assay:

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: Following TUNEL, Pax7 staining was completed as previously reported ( ; ). .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

Immunohistochemistry:

Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
Article Snippet: Paragraph title: Immunohistochemistry: Pax7, laminin, MyHC I ... Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA).

Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
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Article Snippet: Paragraph title: Immunohistochemistry ... After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

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Article Snippet: Paragraph title: Immunohistochemistry ... The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

Amplification:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. Sections were washed 3 × 3 min in PBS and then exposed to a 1 h incubation of Streptavidin‐horseradish peroxidase conjugate (1:100) in PBS, washed 3 × 3 min in PBS, and incubated for 20 min in Alexa Fluor 488 (1:200, (Invitrogen Cat# T20932)) in amplification diluents.

Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
Article Snippet: Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA). .. Utilizing a tyramide signal amplification (TSA) kit (T20932, Life Technologies), slides were incubated for 1 h in streptavidin-horseradish peroxidase (1:100), and then for 20 min in AF488 tyramide (1:200).

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

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Article Snippet: .. Following TBST washes, slides were incubated in biotin goat anti-mouse IgG1 (#115-065-205; Jackson Immuno Research, West Grove, PA, USA) and anti-mouse IgG2a AF488 (#A21121; Invitrogen) secondary antibodies in TBST with 1 % normal goat serum for 1 h. Slides were reacted with streptavidin-horseradish peroxidase included with the TSA kit (#T20935; Invitrogen), incubated in TSA AF594 (#T20950; Invitrogen) in amplification diluents for 15 min, then mounted (#H-1000; Vector Laboratories). .. For staining of mitochondrial COX-1 and microtubule-associated protein light chain 3 (LC3), slides were fixed in 4 % paraformaldehyde for 10 min, washed with TBST (1 % Tween-20), then blocked for 1 h in TBST with 5 % normal goat serum.

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature. .. Visualization of the primary antibody was performed using the Vectastain ABC kit (PK-6100, Vector Laboratories, Burlingame, CA) and Tyramide Signal Amplification (TSA) Plus Cyanine 3 kit (NEL744, PerkinElmer, Waltham, MA, 1:50 in diluent buffer).

Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
Article Snippet: .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies). ..

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: Incubation with anti-Pax7 antibody (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA) was followed by incubation with the biotin-conjugated secondary antibody and streptavidin-HRP included within the Tyramide Signal Amplification kit (#T20935, Invitrogen, Carlsbad, CA). .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

Whole Genome Amplification:

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. For N-acetyl-d-glucosamine staining, muscle sections were fixed with 4% PFA and then incubated with Texas red directly conjugated wheat germ agglutinin (WGA).

Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
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In Situ:

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: TUNEL was detected using In Situ Cell Death, Fluorescein Detection Kit according to manufacturer’s instructions (11684795910, Roche Diagnostics, Mannheim, Germany). .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
Article Snippet: The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies). .. For Pax7/TUNEL/laminin staining, slides were fixed in 4% PFA for 10 min and then incubated in the In Situ Cell Death Detection Kit per manufacturer's instructions.

Software:

Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
Article Snippet: BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Slides were imaged at 20× using an Aperio ScanScope XT digital slide scanner (Leica Biosystems), and cortex was outlined using the Aperio ImageScope software (Leica Biosystems) as previously reported ( ).

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. Myonuclei were manually counted in images captured with a 100–400× total magnification using AxioVision 4.9.1 software to determine the number of myonuclei per fiber.

Microscopy:

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: Paragraph title: Immunofluorescence Microscopy in Mouse Samples ... Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

Plasmid Preparation:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. After a quick wash slides were mounted in medium mounting media (Vector) and allowed to air dry prior to imaging.

Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
Article Snippet: Next, slides were washed in PBS and subsequently blocked in 2.5% normal horse serum (NHS) (Vector, S-2012) for 1 h at room temperature, followed by an overnight incubation (4°C) with Pax7 primary antibody (Pax7-C, 1:100, Developmental Studies Hybridoma Bank, Iowa City, Iowa). .. Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA).

Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
Article Snippet: BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: Ms IgG blocking buffer (Vector #MKB-2231; 1 drop/ml PBS) at RT and then blocked again in 2.5% NHS for 1 h at RT. .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT.

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature. .. Visualization of the primary antibody was performed using the Vectastain ABC kit (PK-6100, Vector Laboratories, Burlingame, CA) and Tyramide Signal Amplification (TSA) Plus Cyanine 3 kit (NEL744, PerkinElmer, Waltham, MA, 1:50 in diluent buffer).

Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
Article Snippet: For cleaved caspase‐3/dystrophin staining, slides were fixed in acetone for 10 min, blocked with 5% normal goat serum (no. S‐1000, Vector) and then incubated overnight in anti‐cleaved caspase‐3 (no. 9579, Cell Signaling, Danvers, MA, USA) and anti‐dystrophin. .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies).

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. Sections were washed again, incubated 30 min in SA-FITC (1:150, Vector # SA-5001), and post-fixed in 4% paraformaldehyde before mounting using Vectashield fluorescent mounting media with DAPI.

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: Following three 3 min washes in PBS, sections were blocked for 1 h in 2.5% normal horse serum (NHS, Vector, S-2012) at RT and subsequently incubated overnight at 4C with primary antibodies diluted in 2.5% NHS against rabbit dystrophin (Santa Cruz #sc-15376, 1:100) and mouse CD68 (Dako #M0814, 1:100). .. On day 2 sections were incubated for 1 h with secondary antibodies: Alexa Fluor 647 conjugated goat anti-rabbit IgG (Invitrogen #21245, 1:500, for dystrophin) and biotin-SP-conjugated goat anti-mouse IgG (Jackson Immuno Research #115-065-205, 1:1000, for CD68) diluted in 2.5% NHS at RT in the dark.

Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
Article Snippet: Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
Article Snippet: The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen). .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

Concentration Assay:

Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

Incubation:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. Sections were washed 3 × 3 min in PBS and then exposed to a 1 h incubation of Streptavidin‐horseradish peroxidase conjugate (1:100) in PBS, washed 3 × 3 min in PBS, and incubated for 20 min in Alexa Fluor 488 (1:200, (Invitrogen Cat# T20932)) in amplification diluents.

Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
Article Snippet: .. Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA). .. Utilizing a tyramide signal amplification (TSA) kit (T20932, Life Technologies), slides were incubated for 1 h in streptavidin-horseradish peroxidase (1:100), and then for 20 min in AF488 tyramide (1:200).

Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

Article Title: Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study
Article Snippet: .. Following TBST washes, slides were incubated in biotin goat anti-mouse IgG1 (#115-065-205; Jackson Immuno Research, West Grove, PA, USA) and anti-mouse IgG2a AF488 (#A21121; Invitrogen) secondary antibodies in TBST with 1 % normal goat serum for 1 h. Slides were reacted with streptavidin-horseradish peroxidase included with the TSA kit (#T20935; Invitrogen), incubated in TSA AF594 (#T20950; Invitrogen) in amplification diluents for 15 min, then mounted (#H-1000; Vector Laboratories). .. For staining of mitochondrial COX-1 and microtubule-associated protein light chain 3 (LC3), slides were fixed in 4 % paraformaldehyde for 10 min, washed with TBST (1 % Tween-20), then blocked for 1 h in TBST with 5 % normal goat serum.

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT. .. Sections were exposed to a 1 h incubation of SA-HRP (1:500 in PBS), washed in 3×3 min intervals in PBS, and incubated for 15 min in Alexa Fluor 568 (Invitrogen # ) in amplification diluents.

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature. .. Visualization of the primary antibody was performed using the Vectastain ABC kit (PK-6100, Vector Laboratories, Burlingame, CA) and Tyramide Signal Amplification (TSA) Plus Cyanine 3 kit (NEL744, PerkinElmer, Waltham, MA, 1:50 in diluent buffer).

Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
Article Snippet: .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies). ..

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. Sections were washed again, incubated 30 min in SA-FITC (1:150, Vector # SA-5001), and post-fixed in 4% paraformaldehyde before mounting using Vectashield fluorescent mounting media with DAPI.

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: .. On day 2 sections were incubated for 1 h with secondary antibodies: Alexa Fluor 647 conjugated goat anti-rabbit IgG (Invitrogen #21245, 1:500, for dystrophin) and biotin-SP-conjugated goat anti-mouse IgG (Jackson Immuno Research #115-065-205, 1:1000, for CD68) diluted in 2.5% NHS at RT in the dark. .. After 3×3 min rinses with PBS, sections were incubated for 1 h with streptavidin-horseradish peroxidase conjugate (SA-HRP, Invitrogen, S-911, 1:500 in PBS) and rinsed again with PBS were repeated following this step.

Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
Article Snippet: .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation. ..

Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
Article Snippet: .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen). .. Slides were co‐stained with DAPI prior to being mounted with Vectashield fluorescent mounting media (Vector Laboratories, Burlingame, CA).

Activity Assay:

Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
Article Snippet: Day 2: Following a wash in PBS, endogenous peroxidase activity was blocked with a 7-min wash in 3% H2 O2 . .. Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA).

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: Following the overnight incubation, muscle sections were subject to epitope retrieval using sodium citrate (10 mM, pH 6.5) at 92 °C for 11 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in PBS followed by an additional blocking step with Mouse-on-Mouse Blocking Reagent (Vector Laboratories, Burlingame, CA). .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

Immunostaining:

Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
Article Snippet: For immunostaining of paraffin-embedded sections, the samples were deparaffinized in xylene and rehydrated in water. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

Mass Spectrometry:

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: Ms IgG blocking buffer (Vector #MKB-2231; 1 drop/ml PBS) at RT and then blocked again in 2.5% NHS for 1 h at RT. .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT.

Permeability:

Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

IA:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: On day 2 sections were incubated in Alexa Fluor 647‐conjugated goat anti‐mouse IgG2b (for MHC I: 1:250, Invitrogen, Carlsbad, CA) and Alexa Fluor 594 goat anti‐rat IgG1 (for laminin: 1:500, Invitrogen, Carlsbad, CA), washed and then incubated with a primary antibody against Pax7 (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA). .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry.

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: Incubation with anti-Pax7 antibody (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA) was followed by incubation with the biotin-conjugated secondary antibody and streptavidin-HRP included within the Tyramide Signal Amplification kit (#T20935, Invitrogen, Carlsbad, CA). .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

Staining:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. Sections were washed 3 × 3 min in PBS and then exposed to a 1 h incubation of Streptavidin‐horseradish peroxidase conjugate (1:100) in PBS, washed 3 × 3 min in PBS, and incubated for 20 min in Alexa Fluor 488 (1:200, (Invitrogen Cat# T20932)) in amplification diluents.

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

Article Title: Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study
Article Snippet: For staining of mitochondrial complex I, subunit 20 and complex IV, subunit 1 (COX-1), slides were fixed in 4 % paraformaldehyde for 10 min, washed with TBST (1 % Tween-20), then blocked for 1 h in TBST with 1 % normal goat serum. .. Following TBST washes, slides were incubated in biotin goat anti-mouse IgG1 (#115-065-205; Jackson Immuno Research, West Grove, PA, USA) and anti-mouse IgG2a AF488 (#A21121; Invitrogen) secondary antibodies in TBST with 1 % normal goat serum for 1 h. Slides were reacted with streptavidin-horseradish peroxidase included with the TSA kit (#T20935; Invitrogen), incubated in TSA AF594 (#T20950; Invitrogen) in amplification diluents for 15 min, then mounted (#H-1000; Vector Laboratories).

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT. .. Sections were exposed to a 1 h incubation of SA-HRP (1:500 in PBS), washed in 3×3 min intervals in PBS, and incubated for 15 min in Alexa Fluor 568 (Invitrogen # ) in amplification diluents.

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: Following TUNEL, Pax7 staining was completed as previously reported ( ; ). .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
Article Snippet: For Pax7/laminin staining, slides were fixed for 10 min in ice‐cold acetone, endogenous peroxidases were blocked with 3% H2 O2 , and then slides were blocked for 1 h in 2.5% normal horse serum. .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies).

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: For dystrophin staining, plantaris muscle sections were rehydrated with PBS and blocked in Mouse-on-Mouse Blocking Reagent. .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
Article Snippet: Histology and immunohistochemistry Histological slides were prepared as 4 μm formalin-fixed sections embedded in paraffin and stained with H & E by the Oncological Sciences Department Histology core facility at Icahn School of Medicine at Mount Sinai. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
Article Snippet: To examine whether DOX administration impacted satellite cell proliferation, we performed Pax7/Ki67 staining. .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

Chloramphenicol Acetyltransferase Assay:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: Sections were incubated for 1 h at RT and then overnight at 4°C with a primary antibody against rabbit anti‐Ki67 (1:100) (Biocare Medical, catCRM325B) and mouse anti‐Pax7 (Concentrate 1:100) from DHSB in 1% TSA at 4°C. .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205).

Binding Assay:

Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
Article Snippet: TSA‐AF555 was used to visualize Pax7 antibody binding. .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

Immunofluorescence:

Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
Article Snippet: Paragraph title: Immunofluorescence Microscopy in Mouse Samples ... Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

Blocking Assay:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: After a 3 × 3 min rinse in PBS, sections were blocked for 1 h in 1% TSA blocking solution (Invitrogen) at RT. .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205).

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: Ms IgG blocking buffer (Vector #MKB-2231; 1 drop/ml PBS) at RT and then blocked again in 2.5% NHS for 1 h at RT. .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT.

Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
Article Snippet: .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. Sections were washed again, incubated 30 min in SA-FITC (1:150, Vector # SA-5001), and post-fixed in 4% paraformaldehyde before mounting using Vectashield fluorescent mounting media with DAPI.

Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
Article Snippet: Sections were incubated for 7 min 3% H₂O₂ in PBS treatment to block endogenous peroxidases. .. On day 2 sections were incubated for 1 h with secondary antibodies: Alexa Fluor 647 conjugated goat anti-rabbit IgG (Invitrogen #21245, 1:500, for dystrophin) and biotin-SP-conjugated goat anti-mouse IgG (Jackson Immuno Research #115-065-205, 1:1000, for CD68) diluted in 2.5% NHS at RT in the dark.

Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
Article Snippet: Peroxidase blocking was completed using endogenous peroxidase solution (Dako Dual Endogenous Enzyme Block #S20003) at room temperature for 15 min. Antibody Diluent (MP Biomedicals Normal Antibody Diluent #980631) was used for serum blocking for 30 min at room temperature. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

Imaging:

Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
Article Snippet: On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. After a quick wash slides were mounted in medium mounting media (Vector) and allowed to air dry prior to imaging.

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    Jackson Immuno biotin sp conjugated affinipure goat anti rabbit igg
    Biotin Sp Conjugated Affinipure Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotin Sp Affinipure Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti rabbit igg/product/Jackson Immuno
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