Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Expression and interaction of RNA binding protein HuR with multidrug transporters (P-GP, MRP1, and ABCG2) in chemoresistant breast and colorectal cancer cells

doi: 10.1016/j.jgeb.2025.100594

Figure Lengend Snippet: ABC transporter and HuR co-expression profiles in chemoresistant cancerous and paired control breast tissues. (A) Immunofluorescence image of ABC transporter and HuR co-expression in chemoresistant breast cancer tissues. (B) Immunofluorescence image of ABC transporter and HuR co-expression in adjacent control tissues. (A) and (B) show the expressions of P-GP, MRP1, and ABCG2 in green and HuR in red, respectively. The scale bar is 25 µm. (C) Correlation of individual P-GP and HuR expression in breast tissues. (D) Correlation of individual MRP1 and HuR expression in breast tissues. (E) Correlation of individual ABCG2 and HuR expression in breast tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HuR expression to total cells. The Y-axis in (C), (D), and (E) denotes the relative ratio of P-GP, MRP1, and ABCG2 expression to total cells, respectively. The slopes indicate the linear relationship between transporters and HuR expression. (F) Comparison of ABC transporter and HuR co-expression in chemoresistant breast cancer and adjacent control tissues. The X-axis represents P-GP and HuR (green), MRP1 and HuR (orange), and ABCG2 and HuR (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HuR co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression.

Article Snippet: The following primary (1°) Abs were used: mouse Anti-HuR monoclonal Ab (ab136542; Abcam; diluted 1:200), rabbit Anti-P-GP polyclonal Ab (ab235954; Abcam; diluted 1:1000), rabbit Anti-MRP1 recombinant monoclonal Ab (ab233383; Abcam; diluted 1:200), and rabbit Anti-ABCG2 recombinant monoclonal Ab (ab229193; Abcam; diluted 1:200).

Techniques: Expressing, Control, Immunofluorescence, Comparison

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Expression and interaction of RNA binding protein HuR with multidrug transporters (P-GP, MRP1, and ABCG2) in chemoresistant breast and colorectal cancer cells

doi: 10.1016/j.jgeb.2025.100594

Figure Lengend Snippet: ABC transporter and HuR co-expression profiles in chemoresistant cancerous and paired control colorectal tissues. (A) Immunofluorescence image of ABC transporter and HuR co-expression in chemoresistant tissues. (B) Immunofluorescence image of ABC transporter and HuR co-expression in adjacent control tissues. (A) and (B) show the expressions of P-GP, MRP1, and ABCG2 in green and HuR in red, respectively. The scale bar is 25 µm. (C) Correlation of individual P-GP and HuR expression in colorectal tissues. (D) Correlation of individual MRP1 and HuR expression in colorectal tissues. (E) Correlation of individual ABCG2 and HuR expression in colorectal tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HuR expression to total cells. The Y-axis in (C), (D), and (E) denotes the relative ratio of P-GP, MRP1, and ABCG2 expression to total cells, respectively. The slopes indicate the linear relationship between transporters and HuR expression. (F) Comparison of ABC transporter and HuR co-expression in chemoresistant and adjacent control tissues. The X-axis represents P-GP and HuR (green), MRP1 and HuR (orange), and ABCG2 and HuR (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HuR co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression.

Article Snippet: The following primary (1°) Abs were used: mouse Anti-HuR monoclonal Ab (ab136542; Abcam; diluted 1:200), rabbit Anti-P-GP polyclonal Ab (ab235954; Abcam; diluted 1:1000), rabbit Anti-MRP1 recombinant monoclonal Ab (ab233383; Abcam; diluted 1:200), and rabbit Anti-ABCG2 recombinant monoclonal Ab (ab229193; Abcam; diluted 1:200).

Techniques: Expressing, Control, Immunofluorescence, Comparison

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Expression and interaction of RNA binding protein HuR with multidrug transporters (P-GP, MRP1, and ABCG2) in chemoresistant breast and colorectal cancer cells

doi: 10.1016/j.jgeb.2025.100594

Figure Lengend Snippet: Molecular docking of RRM1 of HuR with ARE regions from 3ʹUTR of ABC transporters mRNA. (A) Representative docking of HuR RRM1 (AA region: 18–99) and UUUGUUU region from 3ʹUTR of P-GP mRNA (Z-score: −2.5). (B) Representative docking of HuR RRM1 (AA region: 18–99) and UUAUUUUUU region from 3ʹUTR of MRP1 mRNA (Z-score: −2.5). (C) Representative docking of HuR RRM1 (AA region: 18–99) and UUAUUUAAU region from 3ʹUTR of ABCG2 TV1 mRNA (Z-score: −2.5). (D) Representative docking of HuR RRM1 (AA region: 18–99) and UUAUUUUUUUA region from 3ʹUTR of ABCG2 TV2 mRNA (Z-score: −2.6). (E) Representative docking of HuR RRM1 (AA region: 18–99) and AUUUUUUUA region from 3ʹUTR of ABCG2 TV3 mRNA (Z-score: −2.7).

Article Snippet: The following primary (1°) Abs were used: mouse Anti-HuR monoclonal Ab (ab136542; Abcam; diluted 1:200), rabbit Anti-P-GP polyclonal Ab (ab235954; Abcam; diluted 1:1000), rabbit Anti-MRP1 recombinant monoclonal Ab (ab233383; Abcam; diluted 1:200), and rabbit Anti-ABCG2 recombinant monoclonal Ab (ab229193; Abcam; diluted 1:200).

Techniques:

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Expression and interaction of RNA binding protein HuR with multidrug transporters (P-GP, MRP1, and ABCG2) in chemoresistant breast and colorectal cancer cells

doi: 10.1016/j.jgeb.2025.100594

Figure Lengend Snippet: ABC transporter and HuR co-expression profiles in chemoresistant cancerous and paired control breast tissues. (A) Immunofluorescence image of ABC transporter and HuR co-expression in chemoresistant breast cancer tissues. (B) Immunofluorescence image of ABC transporter and HuR co-expression in adjacent control tissues. (A) and (B) show the expressions of P-GP, MRP1, and ABCG2 in green and HuR in red, respectively. The scale bar is 25 µm. (C) Correlation of individual P-GP and HuR expression in breast tissues. (D) Correlation of individual MRP1 and HuR expression in breast tissues. (E) Correlation of individual ABCG2 and HuR expression in breast tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HuR expression to total cells. The Y-axis in (C), (D), and (E) denotes the relative ratio of P-GP, MRP1, and ABCG2 expression to total cells, respectively. The slopes indicate the linear relationship between transporters and HuR expression. (F) Comparison of ABC transporter and HuR co-expression in chemoresistant breast cancer and adjacent control tissues. The X-axis represents P-GP and HuR (green), MRP1 and HuR (orange), and ABCG2 and HuR (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HuR co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression.

Article Snippet: The following primary (1°) Abs were used: mouse Anti-HuR monoclonal Ab (ab136542; Abcam; diluted 1:200), rabbit Anti-P-GP polyclonal Ab (ab235954; Abcam; diluted 1:1000), rabbit Anti-MRP1 recombinant monoclonal Ab (ab233383; Abcam; diluted 1:200), and rabbit Anti-ABCG2 recombinant monoclonal Ab (ab229193; Abcam; diluted 1:200).

Techniques: Expressing, Control, Immunofluorescence, Comparison

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Expression and interaction of RNA binding protein HuR with multidrug transporters (P-GP, MRP1, and ABCG2) in chemoresistant breast and colorectal cancer cells

doi: 10.1016/j.jgeb.2025.100594

Figure Lengend Snippet: ABC transporter and HuR co-expression profiles in chemoresistant cancerous and paired control colorectal tissues. (A) Immunofluorescence image of ABC transporter and HuR co-expression in chemoresistant tissues. (B) Immunofluorescence image of ABC transporter and HuR co-expression in adjacent control tissues. (A) and (B) show the expressions of P-GP, MRP1, and ABCG2 in green and HuR in red, respectively. The scale bar is 25 µm. (C) Correlation of individual P-GP and HuR expression in colorectal tissues. (D) Correlation of individual MRP1 and HuR expression in colorectal tissues. (E) Correlation of individual ABCG2 and HuR expression in colorectal tissues. The X-axis in (C), (D), and (E) denotes the relative ratio of HuR expression to total cells. The Y-axis in (C), (D), and (E) denotes the relative ratio of P-GP, MRP1, and ABCG2 expression to total cells, respectively. The slopes indicate the linear relationship between transporters and HuR expression. (F) Comparison of ABC transporter and HuR co-expression in chemoresistant and adjacent control tissues. The X-axis represents P-GP and HuR (green), MRP1 and HuR (orange), and ABCG2 and HuR (blue) co-expressions. The Y-axis denotes the relative percentage of ABC transporter and HuR co-expression. The Wilcoxon signed-rank test for paired samples was conducted to validate the significance of differences for all types of co-expression.

Article Snippet: The following primary (1°) Abs were used: mouse Anti-HuR monoclonal Ab (ab136542; Abcam; diluted 1:200), rabbit Anti-P-GP polyclonal Ab (ab235954; Abcam; diluted 1:1000), rabbit Anti-MRP1 recombinant monoclonal Ab (ab233383; Abcam; diluted 1:200), and rabbit Anti-ABCG2 recombinant monoclonal Ab (ab229193; Abcam; diluted 1:200).

Techniques: Expressing, Control, Immunofluorescence, Comparison

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Expression and interaction of RNA binding protein HuR with multidrug transporters (P-GP, MRP1, and ABCG2) in chemoresistant breast and colorectal cancer cells

doi: 10.1016/j.jgeb.2025.100594

Figure Lengend Snippet: Molecular docking of RRM1 of HuR with ARE regions from 3ʹUTR of ABC transporters mRNA. (A) Representative docking of HuR RRM1 (AA region: 18–99) and UUUGUUU region from 3ʹUTR of P-GP mRNA (Z-score: −2.5). (B) Representative docking of HuR RRM1 (AA region: 18–99) and UUAUUUUUU region from 3ʹUTR of MRP1 mRNA (Z-score: −2.5). (C) Representative docking of HuR RRM1 (AA region: 18–99) and UUAUUUAAU region from 3ʹUTR of ABCG2 TV1 mRNA (Z-score: −2.5). (D) Representative docking of HuR RRM1 (AA region: 18–99) and UUAUUUUUUUA region from 3ʹUTR of ABCG2 TV2 mRNA (Z-score: −2.6). (E) Representative docking of HuR RRM1 (AA region: 18–99) and AUUUUUUUA region from 3ʹUTR of ABCG2 TV3 mRNA (Z-score: −2.7).

Article Snippet: The following primary (1°) Abs were used: mouse Anti-HuR monoclonal Ab (ab136542; Abcam; diluted 1:200), rabbit Anti-P-GP polyclonal Ab (ab235954; Abcam; diluted 1:1000), rabbit Anti-MRP1 recombinant monoclonal Ab (ab233383; Abcam; diluted 1:200), and rabbit Anti-ABCG2 recombinant monoclonal Ab (ab229193; Abcam; diluted 1:200).

Techniques: