Journal: Biology

Article Title: 3D Breast Cancer Spheroids Reveal Architecture-Dependent HER2 Expression and Signaling

doi: 10.3390/biology14121654

Figure Lengend Snippet: ( A , B ) Biochemical analysis of HER2 downstream signaling pathways. Western blot analysis comparing HER2, phosphorylated HER2-Y1248, pAKT, and pERK levels in 2D versus 3D cultures. B-actin was used as loading control. n = 3 independent experiments. Statistical analysis is performed by t -Student test, * p < 0.05, ** p < 0.01, **** p < 0.0001. No asterisks labeling on graphs indicates no statistically significant differences.

Article Snippet: Western blot membrane of Phospho-HER2 protein detected with anti-phosphoHER2 (Y1248) antibody (#2247, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Protein-Protein interactions, Western Blot, Control, Labeling

Journal: CNS Neuroscience & Therapeutics

Article Title: ErbB2 pY ‐1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification

doi: 10.1111/cns.14407

Figure Lengend Snippet: Western blot analysis of ErbB2 pY‐1248 , ErbB2, and ErbB1 in cellular PD model. (A, C) Representative images of western blot of ErbB2 pY‐1248 , ErbB2, and ErbB1 in extracted protein lysates from SH‐SY5Y cells treated with 6‐OHDA for 6 h. (B, D) Grayscale value analysis of the immunoblots. The band intensity of ErbB2 pY‐1248 and ErbB1 were normalized to ErbB2 and β‐Actin, respectively. It is presented as mean ± SEM. *** p < 0.001 vs. Ctl.

Article Snippet: After blocking with 5% skim milk in TBST for 2 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against β actin (1:5000, A5441, Sigma), ErbB2 pY‐1248 (1:1000, AF1768, R&D Systems), ErbB2 (1:400, sc‐377,344, Santa Cruz Biotechnology), and ErbB1 (1:400, 2232S, Cell Signaling Technology).

Techniques: Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: ErbB2 pY ‐1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification

doi: 10.1111/cns.14407

Figure Lengend Snippet: Western blot analysis of ErbB2 pY‐1248 in mouse PD model. (A) Representative images of western blot of ErbB2 pY‐1248 in the mouse brain tissues. (B) Grayscale value analysis of the immunoblots. The band intensity of ErbB2 pY‐1248 was normalized to β‐Actin. *** p < 0.001 vs. Ctl.

Article Snippet: After blocking with 5% skim milk in TBST for 2 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against β actin (1:5000, A5441, Sigma), ErbB2 pY‐1248 (1:1000, AF1768, R&D Systems), ErbB2 (1:400, sc‐377,344, Santa Cruz Biotechnology), and ErbB1 (1:400, 2232S, Cell Signaling Technology).

Techniques: Western Blot

Journal: Cell reports

Article Title: KRAS G12C -independent feedback activation of wild-type RAS constrains KRAS G12C inhibitor efficacy

doi: 10.1016/j.celrep.2022.110993

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal Anti-phospho-HER2 Y1248 , Cell Signaling Technologies , Cat# 2247S.

Techniques: Derivative Assay, Recombinant, In Vitro, In Vivo, Control, Software