anti pericentrin  (Novus Biologicals)

Journal: Scientific Reports

Article Title: MI-181 enhances ciliation and cilia length in a cigarette smoke exposed airway epithelial model

doi: 10.1038/s41598-026-37296-2

Figure Lengend Snippet: Analysis of MI-181’s effect on FOXJ1 levels in multiciliated cells of ABSC ALI cultures exposed to cigarette smoke. ( a-c ) Same as in Fig. a, except ABSC ALI cultures were fixed and co-stained for pericentrin (anti-pericentrin antibody, green) to visualize multiciliated cells and FOXJ1 (anti-FOXJ1, red) to visualize the levels of FOXJ1. The region of interest (ROI) mask used to quantify FOXJ1 levels are in the rightmost panels. Scale bars indicate 5 μm. ( d-f ) Graphs show a summary of the average total relative fluorescence intensity (RFI) for FOXJ1 (y-axis) for each treatment (x-axis), for each of the indicated donors. n = 75 cell images per condition per donor. Data are represented as the average ± SD. Asterisks indicate statistical significance as *** p < 0.001 compared to the controls. See the Quantification and Statistical Analyses section for details.

Article Snippet: Antibodies used for immunofluorescence were anti-acetylated Tubulin (Cat#T6793 Sigma-Aldrich, Burlington, MA, USA, and Cat#ab179484 Abcam, Waltham, MA, USA), anti-FOXJ1 (Cat#14–9965-82 Thermo Fisher Scientific), and anti-Pericentrin (Cat#NB100-61071 Novus Biologicals, Centennial, CO, USA).

Techniques: Staining, Fluorescence

Journal: Advanced Science

Article Title: Lipid Droplet‐Localized Spindle Apparatus Coiled‐Coil Protein 1 Regulates Lipid Droplet Distribution

doi: 10.1002/advs.202511960

Figure Lengend Snippet: SPDL1‐L‐mediated LD clustering is microtubule‐ and dynein‐dependent. (A) Immunofluorescence staining of pericentrin in HeLa cells expressing mScarlet‐SPDL1‐L following treatment with 100 µM OA. Scale bar represents 10 µm (2 µm for enlarged image). (B) Fluorescence images of HeLa cells transfected with empty vector (EV) or vector expressing full‐length mScarlet‐SPDL1‐L, with or without nocodazole treatment (5 µg/mL), applied 1 h prior to fixation. 100 µM OA was added to induce LD formation. Scale bars represent 10 µm. (C) Fluorescence images of HeLa cells with or without mEGFP‐SPDL1‐L expression during mitosis upon 100 µM OA treatment. Tubulin Tracker Deep Red was used to label microtubules (red) in living cells. Scale bars represent 10 µm. (D) Fluorescence images of HeLa cells expressing full‐length (FL), N‐terminal truncation (∆N: aa 361–622), and point mutants of mScarlet‐SPDL1‐L (∆CCS: A24V, Y60A and F258A) upon 100 µM OA treatment. EV: empty vector control. Scale bars represent 10 µm. (E) Fluorescence microscopy images of HeLa cells co‐expressing mScarlet‐tagged SPDL1‐L and 3×Myc‐tagged p50‐dynamitin, or treated with siRNA targeting dynein heavy chain 1 ( DHC ). The Myc channel shows the localization of Myc‐tagged p50‐dynamitin (green). Scale bars represent 10 µm. (F–H) Statistical analysis of LD clustering in panels B and D ( n ≥ 20). Each dot represents a cell. DAPI labels the nuclei, and LDs are labeled with BODIPY. Data are shown as mean ± SD, with asterisks indicating statistical significance as assessed by one‐way ANOVA and subsequent Tukey's test: ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns: not significant.

Article Snippet: Cells were incubated with primary antibodies diluted in 3% BSA in PBS overnight at 4°C, including anti‐SPDL1‐L (this paper, 1:200), anti‐Lamin B1 (EASYBIO, Cat# BE3191, 1:500), anti‐Hsp60 (Selleck, Cat# F0482, 1:500), anti‐α‐tubulin (EASYBIO, Cat# BE0031, 1:500), and Pericentrin polyclonal antibody (Proteintech, Cat# 27084‐1‐AP, 1:200).

Techniques: Immunofluorescence, Staining, Expressing, Fluorescence, Transfection, Plasmid Preparation, Control, Microscopy, Labeling