Journal: Stem Cell Research & Therapy

Article Title: SMYD1 modulates the proliferation of multipotent cardiac progenitor cells derived from human pluripotent stem cells during myocardial differentiation through GSK3β/β-catenin&ERK signaling

doi: 10.1186/s13287-024-03899-7

Figure Lengend Snippet: Primary and secondary antibodies

Article Snippet: , Anti-p-ERK1/2 , Cell Signaling Technology #4370 , Rabbit , Immunofluorescence , 1:1000.

Techniques: Immunofluorescence, Western Blot, Flow Cytometry, ChIP-chip

Journal: BMC Cancer

Article Title: RPS6KA1 is a histone acetylation-related oncoprotein in acute myeloid leukemia which is targeted by afzelin

doi: 10.1186/s12885-024-12886-3

Figure Lengend Snippet: Afzelin exerts anti-AML effects by acting on the MAPK pathway and RPS6KA1. A . Chemical structure of afzelin. B . HL-60 and THP-1 cells were treated with different concentrations of afzelin (0, 10, 20, 30, 40 and 50 µM) for 24 h, and cell viability was detected by the CCK-8 assay. C-H . The migration (C), invasion (D), cell cycle (E&F), and apoptosis (G&H) of HL-60 and THP-1 cells after treatment with 30 µM afzelin for 24 h were detected by Transwell assay and flow cytometry, respectively. I&J . Protein levels of p-RPS6KA1, p-ERK1/2, p-JNK, p-p38, and p-MCL-1 in HL-60 and THP-1 cells treated with 30 µM afzelin for 24 h were detected by Western blot. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The membrane was then blocked with 5% skim milk at room temperature for 2 h. Subsequently, the PVDF membrane was incubated overnight at 4 °C with the following primary antibodies: anti-RPS6KA1 (ab32114, 1:1000, Abcam), anti-phospho (p)-RPS6KA1 (T359) (ab32413, 1:1000, Abcam), anti-ERK1/2 (ab184699, 1:1000, Abcam), anti-p-ERK1/2 (T202/Y204) (ab278538, 1:1000, Abcam), anti-p-JNK (T183/Y185) (ab307802, 1:1000, Abcam), anti-JNK (ab179461, 1:1000, Abcam), anti-p-p38 (T180) (ab178867, 1:1000, Abcam), anti-p38 (ab170099, 1:1000, Abcam), anti-p-MCL-1 (bs-18726R, Bioss, Beijing, China), and anti-GAPDH (ab9485, 1:1000, Abcam).

Techniques: CCK-8 Assay, Migration, Transwell Assay, Flow Cytometry, Western Blot

Journal: bioRxiv

Article Title: Acrylic Acid Modified Poly-ethylene Glycol Microparticles for Affinity-Based release of Insulin-Like Growth Factor-1 in Neural Applications

doi: 10.1101/2024.09.25.614803

Figure Lengend Snippet: a) Average neurite outgrowth from V2a interneuron neuroaggregates in the presence of 0, 10, 25, 50 or 100 ng/mL IGF-1. (*** = p<0.001, **** = p<0.0001, n=3 for at least 30 neuroaggregates per group). b) Average neurite outgrowth from V2a interneuron neuroaggregates in the presence of 20 ng/mL NT-3, 50 ng/mL IGF-1 or both, normalized to the GF positive control (*** = p<0.001, **** = p<0.0001, n=3 for at least 30 neuroaggregates per group). c) Representative image of tdTomato positive V2a interneuron aggregate in the presence of 50 ng/mL IGF-1. d) Phosphorylation of ERK1/2 in V2a interneuron cell lysates previously exposed to growth factors measured via Western Blot. e) Densitometry of Western Blot data calculated as pERK/ERK and normalized to the control. N=3 (* = p< 0.05, ** = p< 0.01).

Article Snippet: Primary antibodies for Erk1/2 and p-ERK1/2 (Cell Signaling Technology), were used at a 1:1000 dilution.

Techniques: Positive Control, Western Blot, Control