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anti p akt ser473 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p akt ser473 antibody
    Anti P Akt Ser473 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p akt ser473 antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    anti p akt ser473 antibody - by Bioz Stars, 2026-06
    86/100 stars

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    p akt ser473  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc p akt ser473
    L6 myotubes were treated as described in . Immunoblotting (A) and quantified data for p-AKT <t>ser473</t> (B) , S6K1 thr389 (C) , S6 ser235/236 (D) and 4E-BP1 thr37/46 (E) are shown. Total proteins for some of the above signals are shown (F, G) . Myotubes in the two treatment groups were incubated in 1µM of puromycin for 30 minutes and sunset analysis was performed (H) . Data are presented as mean ± SEM, n = 3 independent experiments with 3 technical replicates per experiment, * p < 0.05.
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    L6 myotubes were treated as described in . Immunoblotting (A) and quantified data for p-AKT ser473 (B) , S6K1 thr389 (C) , S6 ser235/236 (D) and 4E-BP1 thr37/46 (E) are shown. Total proteins for some of the above signals are shown (F, G) . Myotubes in the two treatment groups were incubated in 1µM of puromycin for 30 minutes and sunset analysis was performed (H) . Data are presented as mean ± SEM, n = 3 independent experiments with 3 technical replicates per experiment, * p < 0.05.

    Journal: PLOS One

    Article Title: Myofibrillar protein accumulation but reduced protein synthesis in PDCD4-depleted myotubes

    doi: 10.1371/journal.pone.0345305

    Figure Lengend Snippet: L6 myotubes were treated as described in . Immunoblotting (A) and quantified data for p-AKT ser473 (B) , S6K1 thr389 (C) , S6 ser235/236 (D) and 4E-BP1 thr37/46 (E) are shown. Total proteins for some of the above signals are shown (F, G) . Myotubes in the two treatment groups were incubated in 1µM of puromycin for 30 minutes and sunset analysis was performed (H) . Data are presented as mean ± SEM, n = 3 independent experiments with 3 technical replicates per experiment, * p < 0.05.

    Article Snippet: Antibodies to PDCD4 (#9535), p62 (#5114), beclin-1 (#3738), microtubule-associated proteins 1A/1B light chain 3B (LC3B) (#3868), phosphorylated (p) FoxO3a ser253 (#9466), p-AKT Ser473 (#4060), p-S6 Ser235/236 (#4858), and its kinase S6K1 thr389 (#9234) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Western Blot, Incubation

    L6 myotubes were treated with either SCR siRNA (white bars), PDCD4 siRNA (black bars) or PDCD4 siRNA + an AKT inhibitor (grey bars). Immunoblotting (A) and quantified data for p-AKT ser473 (B) , PDCD4 (C) , MHC-1 (D) and troponin (E) and tropomyosin (F) are shown. Data are presented as mean ± SEM, n = 3 independent experiments, * p < 0.05.

    Journal: PLOS One

    Article Title: Myofibrillar protein accumulation but reduced protein synthesis in PDCD4-depleted myotubes

    doi: 10.1371/journal.pone.0345305

    Figure Lengend Snippet: L6 myotubes were treated with either SCR siRNA (white bars), PDCD4 siRNA (black bars) or PDCD4 siRNA + an AKT inhibitor (grey bars). Immunoblotting (A) and quantified data for p-AKT ser473 (B) , PDCD4 (C) , MHC-1 (D) and troponin (E) and tropomyosin (F) are shown. Data are presented as mean ± SEM, n = 3 independent experiments, * p < 0.05.

    Article Snippet: Antibodies to PDCD4 (#9535), p62 (#5114), beclin-1 (#3738), microtubule-associated proteins 1A/1B light chain 3B (LC3B) (#3868), phosphorylated (p) FoxO3a ser253 (#9466), p-AKT Ser473 (#4060), p-S6 Ser235/236 (#4858), and its kinase S6K1 thr389 (#9234) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Western Blot