anti ly6g magnetic beads  (Miltenyi Biotec)

Journal: Journal of Neuroinflammation

Article Title: Interferon-gamma receptor signaling regulates innate immunity during Staphylococcus aureus craniotomy infection

doi: 10.1186/s12974-025-03376-9

Figure Lengend Snippet: IFN-γR1 signaling prevents S. aureus outgrowth during craniotomy infection. Ifngr1 −/− ( n = 16) and WT ( n = 15–17) mice were subjected to craniotomy infection and sacrificed at days 7 and 14 post-infection, where ( A ) bacterial burdens and immune cell ( D-F , J-L ) percentages and ( G-I , M-O ) absolute counts were quantified from the brain, galea, and bone flap. Data pooled from three independent experiments (mean ± SEM) and analyzed by multiple unpaired t -test. ( B-C ) Intracellular bacterial burden in ( B ) Ly6G + granulocytes from the galea and ( C ) F4/80 + microglia/macrophages from the brains of WT and Ifngr1 −/− mice was assessed at day 14 post-infection using gentamicin protection assays. Data are from one experiment (mean ± SD) and analyzed by two-tailed Student’s t-test. Mono, monocyte; MΦ, macrophage; MG, microglia; Bone, bone flap; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001

Article Snippet: PMNs were isolated on the day of experiments from the bone marrow using Ly6G magnetic beads (Cat. # 130-120-337, Miltenyi Biotec, Gaithersburg, MD).

Techniques: Infection, Two Tailed Test

Journal: Infection and Immunity

Article Title: Interleukin-8 Receptor 2 (IL-8R2)-Deficient Mice Are More Resistant to Pulmonary Coccidioidomycosis than Control Mice

doi: 10.1128/IAI.00883-19

Figure Lengend Snippet: PMN are recruited to the lungs in an IL-8R2 (CXCR2)-dependent manner. (A and B) Percentage of Ly6G-high/CD11b-high cells in C.D2 control mice (A) and in IL-8R2 KO mice (B) as determined by flow cytometry. Cells were collected from the BAL fluid of 4 mice in each group at time of necropsy, 14 days after infection. P2 cells had the morphology of neutrophils. P3 cells were not further characterized. (C) Scatterplot depicting the expression of 31 mouse neutrophil-associated genes and Cxcr2 (Table 1 in reference 25; 26) in WT (C.D2) and IL-8R2 KO lungs infected with C. immitis. (D) Relative expression of 31 mouse neutrophil-associated genes after infection in C.D2 and IL-8R2 KO lung tissue. The difference in relative expression was analyzed using a paired t test. Asterisks indicate statistically significant differences (****, P < 0.0001).

Article Snippet: Neutrophils were positively selected from bone marrow cells using anti-Ly6G magnetic coated beads (Miltenyi Biotec, San Diego, CA) according to manufacturer’s instructions.

Techniques: Flow Cytometry, Infection, Expressing