Journal: Frontiers in Immunology

Article Title: Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

doi: 10.3389/fimmu.2023.1156350

Figure Lengend Snippet: Validation levels of anti-Kaiso autoantibodies in the serum using the Meso Scale Discovery (MSD) assay system and the expression of Kaiso in the tissues detected using immunohistochemistry (IHC). (A) MSD analysis of anti-Kaiso autoantibodies in the serum of patients with nr-axSpA (n = 50), AS (n = 40), and RA (n = 40) and healthy controls (HC, n = 45). (B) Synovial and ligament tissues of HC, AS, RA, and nr-axSpA were stained with the anti-Kaiso antibody. Data are presented as mean ± SEM. **P <0.01 compared to patients with nr-axSpA. All IHC images are shown at ×100 magnification and are representative of images from three independent experiments.

Article Snippet: The sections were subsequently incubated overnight with anti-Kaiso antibodies or control IgG (Proteintech, Rosemont, USA).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Frontiers in Immunology

Article Title: Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

doi: 10.3389/fimmu.2023.1156350

Figure Lengend Snippet: ROC curves of anti-Kaiso autoantibody for the discrimination of patients with axSpA from controls. (A) Patients with AxSpA (nr-axSpA and AS) versus healthy controls. (B) Patients with AxSpA versus RA. (C) Patients with nr-axSpA versus healthy controls. (D) Patients with nr-axSpA versus those with RA. (E) Patients with nr-axSpA versus those with AS.

Article Snippet: The sections were subsequently incubated overnight with anti-Kaiso antibodies or control IgG (Proteintech, Rosemont, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

doi: 10.3389/fimmu.2023.1156350

Figure Lengend Snippet: Correlation between anti-Kaiso autoantibody levels, BASDAI scores (A) , CRP (B) , and disease duration (C) in patients with axSpA.

Article Snippet: The sections were subsequently incubated overnight with anti-Kaiso antibodies or control IgG (Proteintech, Rosemont, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

doi: 10.3389/fimmu.2023.1156350

Figure Lengend Snippet: Diagnostic value of anti-Kaiso autoantibody in patients with AS. (A) Levels of anti-Kaiso autoantibody in patients with nr-axSpA progressing to AS over an 8-year period. (B) ROC curves of anti-Kaiso autoantibody for the discrimination of patients with nr-axSpA from those with AS. ***P <0.001.

Article Snippet: The sections were subsequently incubated overnight with anti-Kaiso antibodies or control IgG (Proteintech, Rosemont, USA).

Techniques: Diagnostic Assay

Journal: Frontiers in Immunology

Article Title: Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

doi: 10.3389/fimmu.2023.1156350

Figure Lengend Snippet: Identification of autoantibodies in the serum of patients with nr-axSpA using human proteome (HuProt) microarrays. (A) The Venn diagram shows the number of autoantibodies identified in the serum of patients with nr-axSpA and healthy controls. (B) Representative images of the autoantibody detected in the HuProt microarrays. The arrows indicate the duplicate spots of the antigen protein. (C) High magnification images of the seven specific autoantibodies, including ASAP, BCL7A, EIF2C1, IGHG1, MYLK, SUGT1, and KAISO, identified in patients with nr-axSpA.

Article Snippet: Levels of anti-Kaiso autoantibodies in patients with axSpA and controls were determined using the Meso Scale Discovery assay system.

Techniques:

Journal: Frontiers in Immunology

Article Title: Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

doi: 10.3389/fimmu.2023.1156350

Figure Lengend Snippet: Validation levels of anti-Kaiso autoantibodies in the serum using the Meso Scale Discovery (MSD) assay system and the expression of Kaiso in the tissues detected using immunohistochemistry (IHC). (A) MSD analysis of anti-Kaiso autoantibodies in the serum of patients with nr-axSpA (n = 50), AS (n = 40), and RA (n = 40) and healthy controls (HC, n = 45). (B) Synovial and ligament tissues of HC, AS, RA, and nr-axSpA were stained with the anti-Kaiso antibody. Data are presented as mean ± SEM. **P <0.01 compared to patients with nr-axSpA. All IHC images are shown at ×100 magnification and are representative of images from three independent experiments.

Article Snippet: Levels of anti-Kaiso autoantibodies in patients with axSpA and controls were determined using the Meso Scale Discovery assay system.

Techniques: Expressing, Immunohistochemistry, Staining