anti human tnfrsf10b antibody  (R&D Systems)

Journal: Journal of Extracellular Biology

Article Title: Effects of electric fields on the release and content of extracellular vesicles

doi: 10.1002/jex2.70018

Figure Lengend Snippet: Nanoparticle flowcytometry analysis of TNFRSF10B/CD63 dual labelling extracellular vesicles ( EVs) . ( a) Scatter plots display populations of EVs collected at four different experimental conditions. Each scatter plot is convened into four quarters based on baseline cutoffs on the green or red fluorescent signals. Q1 for TNFRSF10B+ only, Q2 for both TNFRSF10B+ and CD63+, Q3 for CD63+ only, and Q4 for non‐specific nano particles, with the numbers show corresponding percentages of each quarter. ( b) The bar graph compares EV populations, with the height of each bar indicating the percentage for individual EV population (P Q1 , P Q2 and P Q3 ). Samples from the 2nd, 3rd and 4th collections were indicated in blue, orange and grey colours, accordingly ( p < 0.05). P Q1 , P Q2 and P Q3 were the average of the corresponding collections from the 2nd and the 3rd round. Error bars show standard deviation.

Article Snippet: In detail, 100 µL of isolated EV sample was mixed with 4 µL of Alexa488 anti‐human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN, USA) and 2 µL of PE anti‐human CD63 antibody (561925, BD Biosciences, Franklin Lakes, NJ, USA) and incubated at 37°C for 30 min. Unlabelled antibodies were removed using a second application of SEC with a fresh qEV single column (70 nm Gen 2, IZON, Medford, MA).

Techniques: Standard Deviation

Journal: Cells

Article Title: Enhanced Anti-Melanoma Activity of Nutlin-3a Delivered via Ethosomes: Targeting p53-Mediated Apoptosis in HT144 Cells

doi: 10.3390/cells13201678

Figure Lengend Snippet: Nutlin-3a-loaded ethosomes upregulate surface death receptors TRAIL-R2 of p53 wild-type melanoma cells. Surface cytofluorimetric analysis of HT144 expressing p53 wild-type ( A ) and SK-MEL-28 expressing p53 mut ( B ) cell cultures treated with nutlin-3a or nutlin-3a-loaded ethosomes with equivalent nutlin-3a concentrations after 24 h. Vehicle and empty ethosomes are reported as control vehicles. Data are calculated as the fold increase in median fluorescence values with respect to untreated cells (set to 1). Bars represent the mean ± SEM, and each circle denotes the value of each experimental replicate. Statistical analysis was performed by means of ANOVA followed by Bonferroni’s post hoc test. *** indicates a p -value ≤ 0.001.

Article Snippet: Cells were treated for 48 h with nutlin-3a in solution, nutlin-3a-loaded ethosomes, and the vehicle and controls as described above, harvested, and then stained with anti-human PE-conjugated antibody against TRAIL-R2 (clone 71908, R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Control, Fluorescence