anti human plod2 antibody  (Proteintech)

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: Clinicopathological characteristics of patient samples and expression of PLOD2 in laryngeal cancer

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Expressing

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: The primer sequences were used in qRT-PCR

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques:

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: PLOD2, CD44, and CD133 mRNA expression levels and protein in 8 pairs of human LSCC tissues and matched adjacent nontumor tissues. a Relative PLOD2, CD44, and CD133 RNA expression in 8 pairs of LSCC tumor tissues (T) and matched adjacent nontumor tissues (N). b Relative PLOD2, CD44, and CD133 protein expression in human LSCC tumor tissues and matched adjacent nontumor tissues. α-Tubulin was measured as the loading control

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Expressing, RNA Expression

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: IHC analysis of the expression levels of PLOD2, CD44, and CD133 in laryngeal tumor samples with different clinical stages (1, 2, 3 and 4), T stages (T1 & T2, T3 & T4), N stages (N0, N1-N3), and M stages (M0, M1). The expression of PLOD2, CD44 and CD133 was increased in the advanced-stage LSCC tumor tissues. Magnification: × 200

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Expressing

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: Correlation between PLOD2 expression and clinicopathological characteristics of laryngeal cancer patients

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Expressing

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: Univariate and multivariate analysis of factors associated with overall survival in 114 laryngeal cancer patients

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques:

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: OS curves based on different PLOD2 expression levels. a Kaplan-Meier survival curves for LSCC patients with high PLOD2, CD44 and CD133 expression (red line) versus low PLOD2, CD44 and CD133 expression (blue line). b Kaplan-Meier survival curves for LSCC patients with high PLOD2 and CD44/CD133 expression (red line) versus low PLOD2 and CD44/CD133 expression (blue line)

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Expressing

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: Manifestations of PLOD2 maintaining CSC characteristics. a Hep-2 and FaDu cell lines were engineered for the overexpression or silencing of PLOD2. b The SP was analyzed in the different cell lines by multiparametric flow cytometry. c Hep-2 and FaDu cell spheres cultured in medium were photographed; representative images are shown. Scale bar = 50 μm. d The mRNA expression levels of genes representing CSC markers were upregulated. e The protein expression levels of genes representing CSC markers were upregulated. * P < 0.05

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Over Expression, Flow Cytometry, Cell Culture, Expressing

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: PLOD2 could promote drug resistance in laryngeal cancer cells. a Apoptotic scatter plot; Annexin V-FITC and PI staining of the indicated cells treated with DDP (50 μM) for 48 h. b IC50 of DDP in the indicated cells. Each bar represents the mean ± SD of five independent experiments. * P < 0.05. FITC, fluorescein isothiocyanate; PI, propidium iodide

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Staining

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: PLOD2 overexpression contributes to drug resistance in Hep-2 cells in vivo. a Representative photographs of tumors isolated from the experimental mice (with/without DDP treatment). b Cleaved caspase 3 was significantly decreased in PLOD2-overexpressing Hep-2 cells. c Tumor volumes of the experimental mice during the treatment period. Each bar represents the mean ± SD of five independent experiments

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Over Expression, In Vivo, Isolation

Journal: BMC Cancer

Article Title: PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics

doi: 10.1186/s12885-019-6029-y

Figure Lengend Snippet: PLOD2 overexpression could activate the Wnt signaling pathway. a The mRNA levels of downstream genes of the Wnt signaling pathway(c-myc, CD44, Snail, TCF4, and MMP-2) were upregulated by PLOD2. β-Actin was used as an internal control. b Dual luciferase assay showing the effect on TOP/FOP reporter activity in LSCC cells following the overexpression and silencing of PLOD2. c Immunoblot assay of c-myc, β-catenin, MDR-1, and MRP protein levels in LSCC cells transfected with PLOD2 and controls. GAPDH was used as an internal control

Article Snippet: The antibodies used included anti-PLOD2 mouse monoclonal antibody (1:3000 dilution; mab4445, R&D); anti-CD44, anti-CD133, anti-β-catenin rabbit polyclonal antibody (1:3000 dilution; 9582, CST), anti-myc mouse monoclonal antibody (1:3000; AM926, Beyotime), anti-MDR1 rabbit polyclonal antibody (1:3000; BA1351, BOSTER), anti-MRP antibody, anti-caspase-3 mouse monoclonal antibody (1:3000; SC-7272, SANTA), α-tubulin monoclonal mouse monoclonal antibody (1:3000 dilution; T9026, Sigma-Aldrich), and anti-GAPDH antibody (1:3000 dilution; BM1623, BOSTER).

Techniques: Over Expression, Luciferase, Activity Assay, Western Blot, Transfection