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mouse monoclonal igg2b k anti human icam 1  (Becton Dickinson)
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Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Mouse Monoclonal Igg2b K Anti Human Icam 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse igg2a monoclonal anti human icam 1 antibody  (ATCC)
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ATCC mouse igg2a monoclonal anti human icam 1 antibody
Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Mouse Igg2a Monoclonal Anti Human Icam 1 Antibody, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse igg2a monoclonal anti human icam 1 antibody - by Bioz Stars, 2025-01
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monoclonal mouse anti human icam 1 igg antibody  (Santa Cruz Biotechnology)
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Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Monoclonal Mouse Anti Human Icam 1 Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Monoclonal Mouse Anti Human Cd54 Icam 1 Igg1 Leaf, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal igg against human icam 1  (ATCC)
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Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Mouse Monoclonal Igg Against Human Icam 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal igg against human icam 1/product/ATCC
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mouse monoclonal igg against human icam 1 - by Bioz Stars, 2025-01
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mouse anti human monoclonal icam 1 igg  (ATCC)
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ATCC mouse anti human monoclonal icam 1 igg
Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Mouse Anti Human Monoclonal Icam 1 Igg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human monoclonal icam 1 igg - by Bioz Stars, 2025-01
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monoclonal mouse anti human icam 1 igg2b antibodies  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology monoclonal mouse anti human icam 1 igg2b antibodies
Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and <t>membrane-bound</t> <t>ICAM-1</t> protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.
Monoclonal Mouse Anti Human Icam 1 Igg2b Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and membrane-bound ICAM-1 protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.

Journal: Frontiers in Ophthalmology

Article Title: Brief research report: ETS-1 blockade increases ICAM-1 expression in activated human retinal endothelial cells

doi: 10.3389/fopht.2024.1384428

Figure Lengend Snippet: Impact of ETS-1 silencing on the levels of human retinal endothelial cell ICAM1 transcript and membrane-bound ICAM-1 protein. Human retinal endothelial cells were transfected with ETS-1 siRNA s4847, s4848, s4847 plus s4848, or non-targeted control siRNA for 24 hours, and subsequently stimulated for another 24 hours with TNF-α (10 ng/mL), IL-1β (10 ng/mL) or fresh medium alone. (A-C) Graphs show expression of ICAM1 transcript after transfection with (A) ETS-1 siRNA s4847, (B) ETS-1 siRNA s4848, and (C) ETS-1 siRNA s4847 plus s4848, versus the common non-targeted control siRNA. Bars represent mean expression normalized to the expression of reference genes, RPLP0 and GAPDH (n = 3-4 monolayers/condition). Error bars show standard deviation. (D, E) Graph shows membrane-bound ICAM-1 protein after treatment with siRNA, followed by (D) TNF-α or fresh medium, and (E) IL-1β or fresh medium. Bars represent mean relative fluorescence of immunolabelled cell monolayers after corrections for background fluorescence and cell number (n = 4 cell monolayers/condition). Error bars show standard deviation. x = below detectable level. Data were analyzed by (A-C) two-tailed unpaired t-test and (D, E) two-way ANOVA, with Sídák post-hoc testing: *p < 0.05; **p<0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Mouse monoclonal IgG2b K anti-human ICAM-1 (clone LB-2) and isotype-matched negative control anti-dansyl (clone 27-35) antibodies were bought from BD Pharmingen (San Jose, CA), and used at a working concentration of 1 μg/mL.

Techniques: Membrane, Transfection, Control, Expressing, Standard Deviation, Fluorescence, Two Tailed Test