anti human icam 1 mouse igg 1 antibody (Becton Dickinson)
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Anti Human Icam 1 Mouse Igg 1 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human icam 1 mouse igg 1 antibody/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
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1) Product Images from "Interfering ribonucleic acids that suppress expression of multiple unrelated genes"
Article Title: Interfering ribonucleic acids that suppress expression of multiple unrelated genes
Journal: BMC Biotechnology
doi: 10.1186/1472-6750-9-57

Figure Legend Snippet: Design of CODEMIRs targeting VEGF-A and ICAM-1 . (a) Example of seed site alignment and consensus target sequence design for VEGF-A and ICAM-1 using a 12 nucleotide seed. (b) and (c) Schematic illustration of CODEMIR-1 and -2 (respectively) and guide strand binding to the VEGF-A and ICAM-1 mRNAs. Top strand represents the target mRNA (5' to 3'), bottom strand indicates the guide strand (3' to 5').
Techniques Used: Sequencing, Binding Assay

Figure Legend Snippet: Suppression of VEGF-A and ICAM-1 expression by CODEMIR-1 and -2 . Unless otherwise indicated, VEGF-A (ELISA) or ICAM-1 (FACS) were assayed in ARPE-19 cells 48 hours post-transfection and 24 hours post-stimulation with 130 μM Deferoxamine or 1 ng/mL IL-1β respectively. All data points indicate the mean of triplicate samples. Error bars show standard deviation. Statistical significance was determined by two-way ANOVA using a Bonferroni post-test (* p < 0.001 as compared to either untransfected and/or irrelevant siRNA; ** p < 0.001 as compared to untransfected and p < 0.01 as compared to irrelevant siRNA; † p < 0.001 compared to CODEMIR-1). (a) Gene suppression by CODEMIR-1 and -2. (b) Dose responsiveness of gene suppression by CODEMIR-1. Cells were transfected at the indicated concentrations. (ND = not determined). (c) Effect of mismatches on gene suppression by CODEMIR-1. (d) Suppression of VEGF-A and ICAM-1 reporter constructs by CODEMIR-1ARPE-19 cells were co-transfected with the AmCyan reporter and AsRed control plasmids and 40 nM indicated RNA duplexes. Fluorescence was assessed by FACS 48 hours post-transfection. (e) Suppression of VEGF-A and ICAM-1 mRNA expression by selected CODEMIRs. For VEGF-A , stimulation was performed 24 hours post-transfection using 65 μM Deferoxamine and the QuantiGene ® assay was performed on cell lysates prepared 24 hours post-stimulation. For ICAM-1 the QuantiGene ® assay was performed on cell lysates prepared 24 hours post-transfection.
Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Standard Deviation, Construct, Fluorescence

Figure Legend Snippet: VEGF-A and ICAM-1 suppressive activity of 32 variants of CODEMIR-1 . ARPE-19 cells were transfected with 40 nM of the indicated RNA duplex, and VEGF-A (ELISA) and ICAM-1 (FACS) were assayed 48 hours post-transfection and 24 hours after stimulation with 130 μM Deferoxamine or 1 ng/mL IL-1β respectively. The guide strand of each CODEMIR is shown in the 5' to 3' direction, with blue bases indicating mismatches to the VEGF-A target sequence and red bases indicating mismatches to the ICAM-1 target sequence. Data points represent the mean of triplicate samples. Error bars indicate standard deviation.
Techniques Used: Activity Assay, Transfection, Enzyme-linked Immunosorbent Assay, Sequencing, Standard Deviation

Figure Legend Snippet: (a) VEGF and ICAM expression in ARPE-19 cells after transfection with inosine containing CODEMIRs (#100–102) . ARPE-19 cells were transfected with 40 nM duplex RNA and VEGF-A (ELISA) or ICAM-1 (FACS) were assayed 48 hours post-transfection. (b) Comparison of VEGF-A suppressive activity of CODEMIRs containing inosine bases or mismatches at positions 13 and/or 15 of the guide strand. ARPE-19 cells were transfected with 10 nM duplex RNA and VEGF-A (ELISA) was assayed 48 hours post-transfection. Each bar represents the mean of triplicate samples. Error bars indicate standard deviation.
Techniques Used: Expressing, Transfection, Enzyme-linked Immunosorbent Assay, Activity Assay, Standard Deviation

Figure Legend Snippet: Suppression of VEGF-A and ICAM-1 expression by variants (shifted 5' of the guide strand) of CODEMIR-1 . ARPE-19 cells were transfected with 40 nM of the indicated duplex RNA. 24 hours after transfection cells were stimulated with 130 μM Deferoxamine ( VEGF-A ) or 1 ng/ml IL-1β ( ICAM-1 ). Gene expression was assayed 48 hours after transfection by ELISA on cell supernatant (VEGF-A) or FACS (ICAM). All data points indicate the mean of triplicate samples. Error bars indicate standard deviation.
Techniques Used: Expressing, Transfection, Enzyme-linked Immunosorbent Assay, Standard Deviation