annexinv conjugate  (Thermo Fisher)


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    Name:
    Annexin V PE conjugate R PE Annexin V replaces Annexin product AnnexinV04
    Description:
    In collaboration with Nexins Research BV we provide the best and brightest annexin V conjugates available including this R phycoerythrin annexin V conjugate Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine one of the earliest indicators of apoptosis View a selection guide for all annexin V conjugates for flow cytometry Use with the Annexin Binding Buffer for flow cytometry
    Catalog Number:
    a35111
    Price:
    None
    Applications:
    Annexin V Assays|Annexin V Translocation|Apoptosis|Cell Analysis|Cellular Imaging|Flow Cytometry Apoptosis Assays|Flow Cytometry of Cellular Processes|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Flow Cytometry|Cell Viability, Proliferation & Function
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher annexinv conjugate
    In collaboration with Nexins Research BV we provide the best and brightest annexin V conjugates available including this R phycoerythrin annexin V conjugate Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine one of the earliest indicators of apoptosis View a selection guide for all annexin V conjugates for flow cytometry Use with the Annexin Binding Buffer for flow cytometry
    https://www.bioz.com/result/annexinv conjugate/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    annexinv conjugate - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Article Snippet: .. Complement regulatory proteins and annexin V were assessed by flow cytometry with rat-anti-CD46 (Serotec), rat-anti-CD55 (Hycult), rat-anti-CD59 (Serotec) or annexin V (Life Technologies), data collected on FACS Calibur (BD) and analyzed with FlowJo software (FlowJo). .. Bovine POS were incubated with human serum depleted of various complement proteins.

    Article Title: Gingival fibroblasts resist apoptosis in response to oxidative stress in a model of periodontal diseases
    Article Snippet: .. Flow cytometry Gingival fibroblasts were serum starved for 24 h then treated with 1 μ g/ml LPS for 24 h. For the apoptosis assay, cells were harvested and stained with Annexin V (eBioscience, San Diego, CA, USA) and 7-AAD (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions. .. Similarly, ROS activity was quantitated by DCFH-DA staining of serum-starved gingival fibroblasts treated with 1 μ g/ml LPS for 24 h. Cells were cultured with DCFH-DA for 20 min.

    Article Title: HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 S1P3 receptors on vascular endothelium
    Article Snippet: .. Annexin V staining and flow cytometry Cells were detached with TrypLE Express (Gibco), washed and resuspended in Annexin V binding buffer (BD Bioscience). .. Then, cells were stained with PE Annexin V and 7-ADD according manufacturer’s instructions (BD Bioscience) and analyzed in a Cytomics FC500 (Beckman Coulter) flow cytometer.

    Mass Spectrometry:

    Article Title: Rv3033, as an Emerging Anti-apoptosis Factor, Facilitates Mycobacteria Survival via Inhibiting Macrophage Intrinsic Apoptosis
    Article Snippet: .. BMDM cells were seeded into 6-well plates, and adherent monolayers were infected with Ms::Vector or Ms:: Rv3033 (MOI = 10) for 48 h. The apoptotic cells were measured by FACS using an Annexin V/PI kit (eBioscience, San Diego, CA, USA). .. RAW-Vector and RAW-Rv3033 cells were seeded into 6-well plates, and adherent monolayers were infected with BCG (MOI = 10) with or without pretreatment for 1 h with TUDCA (500 μg/ml) for 36 h. The apoptotic cells were measured by FACS using Annexin V-7-AAD kit (eBioscience, San Diego, CA, USA).

    Multiple Displacement Amplification:

    Article Title: Gomisin G Inhibits the Growth of Triple-Negative Breast Cancer Cells by Suppressing AKT Phosphorylation and Decreasing Cyclin D1
    Article Snippet: .. Annexin V and propidium iodide (PI) staining MDA-MB-231 and T47D cells were treated with or without 10 µM of Gomisin G for 24 h. The cells were detached by trypsin-EDTA (Thermo Fisher Scientific). .. The collected cells were washed with FACS buffer (1% FBS in PBS) followed by resuspension in buffer containing annexin V (eBioscience, San Diego, CA, USA) for 15 min at room temperature in the dark.

    Cytometry:

    Article Title: Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Article Snippet: .. Complement regulatory proteins and annexin V were assessed by flow cytometry with rat-anti-CD46 (Serotec), rat-anti-CD55 (Hycult), rat-anti-CD59 (Serotec) or annexin V (Life Technologies), data collected on FACS Calibur (BD) and analyzed with FlowJo software (FlowJo). .. Bovine POS were incubated with human serum depleted of various complement proteins.

    Article Title: Gingival fibroblasts resist apoptosis in response to oxidative stress in a model of periodontal diseases
    Article Snippet: .. Flow cytometry Gingival fibroblasts were serum starved for 24 h then treated with 1 μ g/ml LPS for 24 h. For the apoptosis assay, cells were harvested and stained with Annexin V (eBioscience, San Diego, CA, USA) and 7-AAD (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions. .. Similarly, ROS activity was quantitated by DCFH-DA staining of serum-starved gingival fibroblasts treated with 1 μ g/ml LPS for 24 h. Cells were cultured with DCFH-DA for 20 min.

    Article Title: HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 S1P3 receptors on vascular endothelium
    Article Snippet: .. Annexin V staining and flow cytometry Cells were detached with TrypLE Express (Gibco), washed and resuspended in Annexin V binding buffer (BD Bioscience). .. Then, cells were stained with PE Annexin V and 7-ADD according manufacturer’s instructions (BD Bioscience) and analyzed in a Cytomics FC500 (Beckman Coulter) flow cytometer.

    Infection:

    Article Title: Rv3033, as an Emerging Anti-apoptosis Factor, Facilitates Mycobacteria Survival via Inhibiting Macrophage Intrinsic Apoptosis
    Article Snippet: .. BMDM cells were seeded into 6-well plates, and adherent monolayers were infected with Ms::Vector or Ms:: Rv3033 (MOI = 10) for 48 h. The apoptotic cells were measured by FACS using an Annexin V/PI kit (eBioscience, San Diego, CA, USA). .. RAW-Vector and RAW-Rv3033 cells were seeded into 6-well plates, and adherent monolayers were infected with BCG (MOI = 10) with or without pretreatment for 1 h with TUDCA (500 μg/ml) for 36 h. The apoptotic cells were measured by FACS using Annexin V-7-AAD kit (eBioscience, San Diego, CA, USA).

    FACS:

    Article Title: Rv3033, as an Emerging Anti-apoptosis Factor, Facilitates Mycobacteria Survival via Inhibiting Macrophage Intrinsic Apoptosis
    Article Snippet: .. BMDM cells were seeded into 6-well plates, and adherent monolayers were infected with Ms::Vector or Ms:: Rv3033 (MOI = 10) for 48 h. The apoptotic cells were measured by FACS using an Annexin V/PI kit (eBioscience, San Diego, CA, USA). .. RAW-Vector and RAW-Rv3033 cells were seeded into 6-well plates, and adherent monolayers were infected with BCG (MOI = 10) with or without pretreatment for 1 h with TUDCA (500 μg/ml) for 36 h. The apoptotic cells were measured by FACS using Annexin V-7-AAD kit (eBioscience, San Diego, CA, USA).

    Article Title: Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Article Snippet: .. Complement regulatory proteins and annexin V were assessed by flow cytometry with rat-anti-CD46 (Serotec), rat-anti-CD55 (Hycult), rat-anti-CD59 (Serotec) or annexin V (Life Technologies), data collected on FACS Calibur (BD) and analyzed with FlowJo software (FlowJo). .. Bovine POS were incubated with human serum depleted of various complement proteins.

    Apoptosis Assay:

    Article Title: Gingival fibroblasts resist apoptosis in response to oxidative stress in a model of periodontal diseases
    Article Snippet: .. Flow cytometry Gingival fibroblasts were serum starved for 24 h then treated with 1 μ g/ml LPS for 24 h. For the apoptosis assay, cells were harvested and stained with Annexin V (eBioscience, San Diego, CA, USA) and 7-AAD (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions. .. Similarly, ROS activity was quantitated by DCFH-DA staining of serum-starved gingival fibroblasts treated with 1 μ g/ml LPS for 24 h. Cells were cultured with DCFH-DA for 20 min.

    Expressing:

    Article Title: Inhibition of ER stress-mediated apoptosis in macrophages by nuclear-cytoplasmic relocalization of eEF1A by the HIV-1 Nef protein
    Article Snippet: .. MDMs were treated with rNef 48 h post-transfection, and eEF1A, rNef, Exp-t, Exp-1 and Exp-5 protein expression and MDM apoptosis analyzed by western blot and annexin-V (Invitrogen) assay, respectively. .. Transfection efficiency was monitored using a fluorescein-conjugated scrambled control duplex, exceeding 80% in MDMs.

    Staining:

    Article Title: Potential Role of HPA Axis and Sympathetic Nervous Responses in Depletion of B Cells Induced by H9N2 Avian Influenza Virus Infection
    Article Snippet: .. Briefly, 0.1 ml of cell suspensions containing 106 cells was suspended in staining buffer (including 100 µg/ml RNase A) and stained with annexin-V, PI (Invitrogen) and anti-B220 according to the manufacturer's instructions. .. In this case, 10,000 events gated for B220+ B cells were collected, and histograms were analyzed using MultiCycle AV software.

    Article Title: Gomisin G Inhibits the Growth of Triple-Negative Breast Cancer Cells by Suppressing AKT Phosphorylation and Decreasing Cyclin D1
    Article Snippet: .. Annexin V and propidium iodide (PI) staining MDA-MB-231 and T47D cells were treated with or without 10 µM of Gomisin G for 24 h. The cells were detached by trypsin-EDTA (Thermo Fisher Scientific). .. The collected cells were washed with FACS buffer (1% FBS in PBS) followed by resuspension in buffer containing annexin V (eBioscience, San Diego, CA, USA) for 15 min at room temperature in the dark.

    Article Title: Gingival fibroblasts resist apoptosis in response to oxidative stress in a model of periodontal diseases
    Article Snippet: .. Flow cytometry Gingival fibroblasts were serum starved for 24 h then treated with 1 μ g/ml LPS for 24 h. For the apoptosis assay, cells were harvested and stained with Annexin V (eBioscience, San Diego, CA, USA) and 7-AAD (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions. .. Similarly, ROS activity was quantitated by DCFH-DA staining of serum-starved gingival fibroblasts treated with 1 μ g/ml LPS for 24 h. Cells were cultured with DCFH-DA for 20 min.

    Western Blot:

    Article Title: Inhibition of ER stress-mediated apoptosis in macrophages by nuclear-cytoplasmic relocalization of eEF1A by the HIV-1 Nef protein
    Article Snippet: .. MDMs were treated with rNef 48 h post-transfection, and eEF1A, rNef, Exp-t, Exp-1 and Exp-5 protein expression and MDM apoptosis analyzed by western blot and annexin-V (Invitrogen) assay, respectively. .. Transfection efficiency was monitored using a fluorescein-conjugated scrambled control duplex, exceeding 80% in MDMs.

    Binding Assay:

    Article Title: HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 S1P3 receptors on vascular endothelium
    Article Snippet: .. Annexin V staining and flow cytometry Cells were detached with TrypLE Express (Gibco), washed and resuspended in Annexin V binding buffer (BD Bioscience). .. Then, cells were stained with PE Annexin V and 7-ADD according manufacturer’s instructions (BD Bioscience) and analyzed in a Cytomics FC500 (Beckman Coulter) flow cytometer.

    Software:

    Article Title: Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Article Snippet: .. Complement regulatory proteins and annexin V were assessed by flow cytometry with rat-anti-CD46 (Serotec), rat-anti-CD55 (Hycult), rat-anti-CD59 (Serotec) or annexin V (Life Technologies), data collected on FACS Calibur (BD) and analyzed with FlowJo software (FlowJo). .. Bovine POS were incubated with human serum depleted of various complement proteins.

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  • 93
    Thermo Fisher anti human cytokeratine 18 fitc prelabeled monoclonal mouse flow cytometry antibody
    Anti Human Cytokeratine 18 Fitc Prelabeled Monoclonal Mouse Flow Cytometry Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cytokeratine 18 fitc prelabeled monoclonal mouse flow cytometry antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human cytokeratine 18 fitc prelabeled monoclonal mouse flow cytometry antibody - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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