Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: Cell proliferation analysis. CCK-8 assay ( A ) and Colony formation assay ( B ) of SW480/SW620 cells transfected with PARP1, PARP1/RNAi, and Vector/Scramble. C Western blot analysis of cyclin D1 and p21 expression. D QPCR analysis of cyclin D1 and p21 expression. Blot membranes were cut prior to hybridisation with antibodies during blotting and developed with X-ray film. *p < 0.05 , (Student’s t test)

Article Snippet: To detect specific proteins, primary antibodies that were used included α-Tubulin mouse monoclonal antibody (1:1000; Sigma-Aldrich, St. Louis, MO, USA), anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam), anti-human cyclin D1 rabbit monoclonal antibody (1:1500; Abcam), and anti-human P21 rabbit monoclonal antibody (1:1500; Abcam).

Techniques: CCK-8 Assay, Colony Assay, Transfection, Plasmid Preparation, Western Blot, Expressing, Hybridization

Journal: Cell Reports Medicine

Article Title: Comprehensive proteogenomic characterization of rare kidney tumors

doi: 10.1016/j.xcrm.2024.101547

Figure Lengend Snippet:

Article Snippet: Rabbit Monoclonal IgG Human Cyclin D1 , Cell Marque , Catalog: 241R-18 RRID: AB_1158233.

Techniques: Software

Journal: Oncotarget

Article Title: Cyclin D1 expression in penile cancer

doi: 10.18632/oncotarget.28584

Figure Lengend Snippet: Association of cyclin D1 regarding clinical-histopathological features

Article Snippet: For IHC analysis, a rabbit monoclonal antibody anti-human-cyclin D1 (Clone EP12, Dako), purchased from Agilent (Santa Clara, CA, USA) was used.

Techniques: In Situ, Transformation Assay

Journal: Oncotarget

Article Title: Cyclin D1 expression in penile cancer

doi: 10.18632/oncotarget.28584

Figure Lengend Snippet: Analysis of disease-free survival of patients according to the presence and absence of cyclin D1 (Log-Rank p = 0.669).

Article Snippet: For IHC analysis, a rabbit monoclonal antibody anti-human-cyclin D1 (Clone EP12, Dako), purchased from Agilent (Santa Clara, CA, USA) was used.

Techniques:

Journal: Cell Cycle

Article Title: Altered expression of G1/S phase cell cycle regulators in placental mesenchymal stromal cells derived from preeclamptic pregnancies with fetal-placental compromise

doi: 10.1080/15384101.2016.1261766

Figure Lengend Snippet: JunB and Cyclin D1 gene and protein expression levels in physiological placental villous explants treated with culture media conditioned by normal or PE-PDMSC. (A)JunB mRNA (left panels) and protein (right panels) expression levels in physiological villous explants treated with unconditioned media (CTRL, n = 16 explants) or media conditioned by normal (N CM, n = 16 explants) and preeclamptic (PE CM, n = 16 explants) PDMSCs as assessed by Real Time PCR and Western Blot analysis. B) Cyclin D1 mRNA (left panels) and protein (right panels) expression levels in physiological villous explants treated with unconditioned media (CTRL, n = 16 explants) or media conditioned by normal (N CM, n = 16 explants) and preeclamptic (PE CM, n = 16 explants) PDMSCs as assessed by Real Time PCR and Western Blot analysis. Statistical significance (*) has been considered as p < 0.05.

Article Snippet: Primary antibodies were: rabbit polyclonal anti-human JunB (1:2000 dilution, Merk-Millipore, Cat. No. 07–1333), mouse monoclonal anti-human Cyclin D1 (1:1500 dilution, Cell Signaling, Cat. No. 2978), rabbit monoclonal anti-human p16 INK4A (1:250 dilution, Cell Signaling, Cat.No 4824), mouse monoclonal anti-human p18 INK4C (1:250 dilution, Cell Signaling, Cat.No 2896), mouse monoclonal anti-human CDK4 (1:500 dilution, Cell Signaling, Cat.No 12790), mouse monoclonal anti-human CDK6 (1:500 dilution, Cell Signaling, Cat. No. 3136), mouse monoclonal anti-human PARP1 (1:500 diluition, Abcam, Cat. No. ab110915) and mouse monoclonal anti-human β-actin (1:1000, Sigma-Aldrich, Cat.No.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell Cycle

Article Title: Altered expression of G1/S phase cell cycle regulators in placental mesenchymal stromal cells derived from preeclamptic pregnancies with fetal-placental compromise

doi: 10.1080/15384101.2016.1261766

Figure Lengend Snippet: Cyclin D1 spatial localization in physiological placental villous explants treated with culture media conditioned by normal or PE-PDMSC. (A) Absence of positive immunoreactivity for Cyclin D1 in section stained with control IgG. B) Cyclin D1 spatial localization in physiological villous explants treated with unconditioned media (CTRL, n = 4 explants) assessed by immunofluorescent staining. C) Cyclin D1 spatial localization in physiological villous explants treated with media conditioned by normal (N CM, n = 4 explants) assessed by immunofluorescent staining. C) Cyclin D1 spatial localization in physiological villous explants treated media conditioned by with preeclamptic (PE CM, n = 4 explants) assessed by immunofluorescent staining. Cell nuclei are showed in blue by DAPI signal. TR, trophoblast cells; M, mesenchyme; V, vessel. Original magnifications, x40.

Article Snippet: Primary antibodies were: rabbit polyclonal anti-human JunB (1:2000 dilution, Merk-Millipore, Cat. No. 07–1333), mouse monoclonal anti-human Cyclin D1 (1:1500 dilution, Cell Signaling, Cat. No. 2978), rabbit monoclonal anti-human p16 INK4A (1:250 dilution, Cell Signaling, Cat.No 4824), mouse monoclonal anti-human p18 INK4C (1:250 dilution, Cell Signaling, Cat.No 2896), mouse monoclonal anti-human CDK4 (1:500 dilution, Cell Signaling, Cat.No 12790), mouse monoclonal anti-human CDK6 (1:500 dilution, Cell Signaling, Cat. No. 3136), mouse monoclonal anti-human PARP1 (1:500 diluition, Abcam, Cat. No. ab110915) and mouse monoclonal anti-human β-actin (1:1000, Sigma-Aldrich, Cat.No.

Techniques: Staining

Journal: Stem Cells International

Article Title: Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibit Endometrial Cancer Cell Proliferation and Migration through Delivery of Exogenous miR-302a

doi: 10.1155/2019/8108576

Figure Lengend Snippet: Mechanism of action of miR-302a. (a, b) Expression levels of cyclin D1 and AKT in ISK and ECC-1 cells after indicated treatment was measured by quantitative RT-PCR analyses ( ∗∗ P < 0.01). (c) Expression levels of cyclin D1, AKT, and p-AKT in ISK and ECC-1 cells after indicated treatment was determined by western blot analyses. (d, e) The mRNA and protein expression of cyclin D1 in endometrial cancer tissues ( n = 6) and adjacent tissues ( n = 6) ( ∗ P < 0.05, ∗∗ P < 0.01). (f) Representative image of cyclin D1 expression in EC samples and adjacent tissues was determined by IHC.

Article Snippet: The sections were incubated with rabbit anti-human monoclonal cyclin D1 antibody (1 : 1000, Abcam) overnight at 4°C and goat anti-rabbit HRP secondary antibody (1 : 500, Abcam) 37°C for 30 minutes.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: MicroRNA-365 Inhibits Cell Growth and Promotes Apoptosis in Melanoma by Targeting BCL2 and Cyclin D1 (CCND1)

doi: 10.12659/MSM.909633

Figure Lengend Snippet: MiR-365 targeted BCL2 and CCND1. A375 and A2058 were transfected with 100 nM miR-365 mimics or control oligos for 48 hours. ( A ) mRNA levels of BCL2 and CCND1 were measured by qRT-PCR. ( B ) Protein levels of BCL2 and CCND1 were measured using western blot. ( C ) An illustration of the targeting sites of miR-365 on 3′UTR of BCL2 and CCND1 and the constructs of the luciferase reporter plasmids. ( D ) The dual-luciferase reporter assay was performed to show that BCL2 and CCND1 were directly targeted by miR-365. A375 cells were co-transfected with indicated oligos and constructs. The Renilla and firefly luciferase activity was then measured using the dual-luciferase kit as instructed by the manufacture’s protocol. Data shown are the mean ±SEM of three independent experiments. * P <0.05, ** P <0.01, and ns, no significance.

Article Snippet: After blocked by 5% non-fat milk for 1 hour at room temperature, the membranes were incubated with rabbit anti-human BCL2 or rabbit anti-human CCND1 (both 1: 1000, Cell Signaling, USA) and mouse anti-human β-actin (1: 2000, Abcam, USA) for overnight at 4°C.

Techniques: Transfection, Quantitative RT-PCR, Western Blot, Construct, Luciferase, Reporter Assay, Activity Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: MicroRNA-365 Inhibits Cell Growth and Promotes Apoptosis in Melanoma by Targeting BCL2 and Cyclin D1 (CCND1)

doi: 10.12659/MSM.909633

Figure Lengend Snippet: The effects of miR-365 were partially mediated through targeting BCL2 and CCND1. ( A ) A375 and A2058 cells were co-transfected with miR-365 mimic oligos and a construct encoding CCND1 or an empty vector. Cell cycle was analyzed using the PI staining flow cytometry. ( B ) A375 and A2058 cells were co-transfected with miR-365 mimic oligos and a construct encoding BCL2 or an empty vector. Cell apoptosis was measured by using Annexin V-FITC/Hoechst 33258 double staining flow cytometry. Data shown are the mean ±SEM of 3 independent experiments. * P <0.05, ** P <0.01, and ns, no significance.

Article Snippet: After blocked by 5% non-fat milk for 1 hour at room temperature, the membranes were incubated with rabbit anti-human BCL2 or rabbit anti-human CCND1 (both 1: 1000, Cell Signaling, USA) and mouse anti-human β-actin (1: 2000, Abcam, USA) for overnight at 4°C.

Techniques: Transfection, Construct, Plasmid Preparation, Staining, Flow Cytometry, Double Staining

Journal: Stem Cells International

Article Title: Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

doi: 10.1155/2016/7532798

Figure Lengend Snippet: 5 μ M EGCG rescues the inhibition of osteogenic differentiation induced by 0.2 mM H 2 O 2 involved in the Wnt pathway. (a) Western blot analysis of Wnt pathway-related regulators β -catenin and cyclin D1 in the cultures of experimental groups. (b) Relative protein expressions normalized to GAPDH in (a). Wnt inhibitor DKK-1 blocked the protective effects of EGCG on the inhibition of osteogenic differentiation by 0.2 mM H 2 O 2 exposure, as evidenced by ALP activity (c), calcium contents (d), and relative mRNAs expression of Runx2 and OSX (e). Data were presented as mean ± SEM. ∗ p < 0.01 and # p < 0.05 versus control, & p < 0.01 versus 0.2 mM H 2 O 2 treatment alone in (b), and $ p < 0.01 versus cotreatment of 0.2 mM H 2 O 2 and 0.1 μ M EGCG.

Article Snippet: Separated proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad), which were then blocked with 5% (w/v) skim milk in Tris-buffered saline containing 0.1% (v/v) Tween 20, and incubated with commercial primary antibodies (1 : 1,000 dilution; all purchased from Abcam, UK): mouse monoclonal anti-human GAPDH, rabbit monoclonal anti-human β -catenin, and rabbit monoclonal anti-human cyclin D1.

Techniques: Inhibition, Western Blot, Activity Assay, Expressing