Journal: PLoS ONE

Article Title: Acidic Fibroblast Growth Factor Promotes Endothelial Progenitor Cells Function via Akt/FOXO3a Pathway

doi: 10.1371/journal.pone.0129665

Figure Lengend Snippet: (A) Monolayer of first passage EPCs with a cobblestone morphology (magnification×100). (B) Immunofluorescence staining of EPCs double positive for Dil-AcLDL and FITC-UEA-I (magnification×400). (C) Immunofluorescence staining of EPCs positive for CD34, CD133 and VEGFR-2 (magnification×200). (D) A representative FSC/SSC plot and the expression of EPC markers (CD34, CD133 and VEGFR-2) analyzed with flow cytometry. Data are mean ± SD, n = 3.

Article Snippet: For EPC identification, cells were immunofluorescently stained using antibodies against endothelial markers CD34 (Epitomics) and VEGFR-2 (R&D Systems) as well as stem/progenitor marker CD133 (Origene).

Techniques: Immunofluorescence, Staining, Expressing, Flow Cytometry

Journal: Iranian Journal of Pathology

Article Title: Cyclin D1 Expression in Patients with Laryngeal Squamous Cell Carcinoma

doi: 10.30699/ijp.2020.116579.2276

Figure Lengend Snippet: Frequency distribution of CD1 protein expression in patients with laryngeal squamous cell carcinoma according to the age and gender

Article Snippet: The slides were then incubated with anti-human CD1 monoclonal rabbit antibody (Dako Autostainer/ Autostainer Plus, USA) in a humidified chamber at 4°C overnight.

Techniques: Expressing

Journal: Iranian Journal of Pathology

Article Title: Cyclin D1 Expression in Patients with Laryngeal Squamous Cell Carcinoma

doi: 10.30699/ijp.2020.116579.2276

Figure Lengend Snippet: Frequency distribution of CD1 protein expression in patients with laryngeal squamous cell carcinoma according to the smoking, location of the tumor and the geographical region

Article Snippet: The slides were then incubated with anti-human CD1 monoclonal rabbit antibody (Dako Autostainer/ Autostainer Plus, USA) in a humidified chamber at 4°C overnight.

Techniques: Expressing, Northern Blot

Journal: Iranian Journal of Pathology

Article Title: Cyclin D1 Expression in Patients with Laryngeal Squamous Cell Carcinoma

doi: 10.30699/ijp.2020.116579.2276

Figure Lengend Snippet: Frequency distribution of CD1 protein expression in patients with laryngeal squamous cell carcinoma according to the grade and stage of the tumor

Article Snippet: The slides were then incubated with anti-human CD1 monoclonal rabbit antibody (Dako Autostainer/ Autostainer Plus, USA) in a humidified chamber at 4°C overnight.

Techniques: Expressing

Journal: The Journal of Immunology Author Choice

Article Title: T Cells Specific for a Mycobacterial Glycolipid Expand after Intravenous Bacillus Calmette–Guérin Vaccination

doi: 10.4049/jimmunol.2001065

Figure Lengend Snippet: GMM is presented by CD1b to T cells in macaques. (A) Evolutionary genetics of group 1 CD1 genes among primates. CD1a, CD1b, and CD1c sequences were collated, aligned, and compared as described in the Materials and Methods. Amino acid residues under positive selection were modeled onto H. sapiens CD1 crystal structures using PyMOL v2.4 and are noted in red. (Protein Data Bank numbers 5J1A, 5L2J, and 3OV6 for CD1a, CD1b, and CD1c, respectively). (B) H. sapiens CD1b-GMM and Mamu CD1b-GMM tetramers were used to identify GMM-specific T cells present within a human T cell line (G02) by staining with both GMM-loaded (BV421) and mock-loaded (FITC) CD1b tetramers. Mean fluorescence intensities (MFI) of populations identified by H. sapiens and Mamu CD1b tetramers are compared using overlapping histograms. (C) GMM-specific T cells present within G02 were identified by staining with either H. sapiens CD1b-GMM or Mamu CD1b-GMM tetramers, and TCR genes expressed by GMM-specific T cells were identified using Abs targeting TRAV1-2 and TRBV4-1. (D) PBMC from an Indian-origin rhesus macaque that was vaccinated intradermally with BCG were stained with Mamu CD1b-GMM and Mamu CD1b mock-loaded tetramers, sorted, and expanded in vitro as described in the Materials and Methods. The resulting T cell line (bG52R) was restained with Mamu CD1b-GMM and Mamu CD1b mock-loaded tetramer. (E) The species origin of bG52R was confirmed by staining with an anti-CD3ε Ab (clone UCHT1) that does not bind with Mamu CD3ε as well as an anti-CD3ε Ab (clone SP34-2) that binds CD3ε from both species. (F) T cells staining with Mamu CD1b-GMM tetramer present within bG52R were further examined for coreceptor expression using Abs targeting CD4 and CD8α. (G) bG52R was simultaneously stained with Mamu CD1b-GMM tetramer (BV421) and H. sapiens CD1b-GMM tetramer (allophycocyanin). Data are representative of at least two independent experiments. See also Supplemental Fig. 1 and Table I.

Article Snippet: Cells were screened by flow cytometry for CD1 expression by staining with the following anti-human CD1 Abs: anti-CD1a PE (clone HI149), anti-CD1b FITC (clone SN13), anti-CD1c allophycocyanin (clone L161) (BioLegend), and anti-CD1d PE (clone 42.1) (BD Biosciences).

Techniques: Selection, Staining, Fluorescence, In Vitro, Expressing

Journal: The Journal of Immunology Author Choice

Article Title: T Cells Specific for a Mycobacterial Glycolipid Expand after Intravenous Bacillus Calmette–Guérin Vaccination

doi: 10.4049/jimmunol.2001065

Figure Lengend Snippet: GMM is presented by CD1c to T cells in macaques. (A) PBMC from an Indian-origin rhesus macaque that was vaccinated intradermally with BCG were stained with Mamu CD1c-GMM (PE) and Mamu CD1c mock-loaded (BV421) tetramers to identify CD1c-GMM–specific T cells. (B) T cells staining with Mamu CD1c-GMM but not Mamu CD1c mock-loaded tetramer were sorted and expanded in vitro for 2 wk to generate a T cell line (cG52R). (C) The species origin of cG52R was confirmed by staining with an anti-CD3ε Ab (clone UCHT1) that does not bind Mamu CD3ε as well as an anti-CD3ε Ab (clone SP34-2) that binds CD3ε from both species. (D) T cells staining with Mamu CD1c-GMM tetramer present within cG52R were further examined for coreceptor expression using Abs targeting CD4 and CD8α. (E) cG52R was simultaneously stained with Mamu CD1c-GMM tetramer (G780) and H. sapiens CD1c-GMM tetramer (PE). Fewer than 5% of T cells staining with Mamu CD1c-GMM tetramer costain with H. sapiens CD1c-GMM tetramer. (F) cG52R was coincubated with K562-CD1c or K562-EV APCs in the presence or absence of GMM. IFN-γ detection by ELISPOT demonstrates the Mamu-derived T cell line is partially activated by H. sapiens CD1 proteins. Data are representative of at least three (A–E) or two (F) independent experiments.

Article Snippet: Cells were screened by flow cytometry for CD1 expression by staining with the following anti-human CD1 Abs: anti-CD1a PE (clone HI149), anti-CD1b FITC (clone SN13), anti-CD1c allophycocyanin (clone L161) (BioLegend), and anti-CD1d PE (clone 42.1) (BD Biosciences).

Techniques: Staining, In Vitro, Expressing, Enzyme-linked Immunospot, Derivative Assay