full length hdac 7  (Millipore)


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    Structured Review

    Millipore full length hdac 7
    The non-cleavable caspase-8/FLIPL heterodimer has attenuated activity on most apoptotic substrates but high activity on <t>HDAC-7</t>
    Full Length Hdac 7, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length hdac 7/product/Millipore
    Average 86 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    full length hdac 7 - by Bioz Stars, 2020-04
    86/100 stars

    Images

    1) Product Images from "FLIPL induces caspase-8 activity in the absence of interdomain caspase-8 cleavage and alters substrate specificity"

    Article Title: FLIPL induces caspase-8 activity in the absence of interdomain caspase-8 cleavage and alters substrate specificity

    Journal: The Biochemical journal

    doi: 10.1042/BJ20101738

    The non-cleavable caspase-8/FLIPL heterodimer has attenuated activity on most apoptotic substrates but high activity on HDAC-7
    Figure Legend Snippet: The non-cleavable caspase-8/FLIPL heterodimer has attenuated activity on most apoptotic substrates but high activity on HDAC-7

    Techniques Used: Activity Assay

    Related Articles

    Diagnostic Assay:

    Article Title: Modulation of SIV and HIV DNA Vaccine Immunity by Fas-FasL Signaling
    Article Snippet: Paragraph title: 2.2. Peptides, Antibodies, and Diagnostic Kits for Flow Cytometry and Western Blots ... Rabbit polyclonal antibodies for FADD (AV30294) and FLIP (F9800) were ordered from Sigma-Aldrich.

    Flow Cytometry:

    Article Title: Modulation of SIV and HIV DNA Vaccine Immunity by Fas-FasL Signaling
    Article Snippet: Paragraph title: 2.2. Peptides, Antibodies, and Diagnostic Kits for Flow Cytometry and Western Blots ... Rabbit polyclonal antibodies for FADD (AV30294) and FLIP (F9800) were ordered from Sigma-Aldrich.

    Protease Inhibitor:

    Article Title: PU.1 supports TRAIL-induced cell death by inhibiting NF-κB-mediated cell survival and inducing DR5 expression
    Article Snippet: The primary antibodies used were anti-PU.1 (no. 2258; Cell Signaling, Switzerland or sc-365208; Santa Cruz, Switzerland), anti-cFLIP (F9800; Sigma) and (AG-20B-0056; Adipogen AG, Switzerland), anti-XIAP (M044-3; MBL, Switzerland), anti-caspase-3 (235412; Calbiochem, Switzerland) and (no. 9661; Cell Signaling), anti-PARP (nos 9542, 9541; Cell Signaling) anti-cleaved caspase-8 (no. 9496; Cell Signaling), anti-caspase-8 (ALX-804-242; Enzo Life Sciences (ELS) AG, Switzerland), anti-Bcl-2 (SAB4500003; Sigma), anti-Mcl-1 (559027; BD Biosciences, Switzerland), anti-DR5 (no. 8074; Cell Signaling), anti-DcR2 (no. 8049; Cell Signaling), anti-p65 (no. 8242; Cell Signaling), anti-phospho-p65 (no. 3033; Cell Signaling), anti-I κ B α (no. 4814; Cell Signaling) anti-phospho-I κ B α (no. 2859; Cell Signaling), anti-RIPK1 (no. 3493; Cell Signaling) anti- α -tubulin (no. 3873; Cell Signaling, anti-Myc tag (ab13836; Abcam), anti-FLAG tag (no. 2368; Cell Signaling)) and anti-histone H3 (no. 9715; Cell Signaling). .. For immunoblotting, cells were lysed in urea buffer (40 μ l/5 × 106 cells) consisting of 8M urea and 0.5% (v/v) Triton X-100 supplemented with Protease Inhibitor Cocktail (Roche Diagnostics (Schweiz) AG, Switzerland).

    Cytometry:

    Article Title: Modulation of SIV and HIV DNA Vaccine Immunity by Fas-FasL Signaling
    Article Snippet: Paragraph title: 2.2. Peptides, Antibodies, and Diagnostic Kits for Flow Cytometry and Western Blots ... Rabbit polyclonal antibodies for FADD (AV30294) and FLIP (F9800) were ordered from Sigma-Aldrich.

    Produced:

    Article Title: High Distribution of CD40 and TRAF2 in Th40 T Cell Rafts Leads to Preferential Survival of this Auto-Aggressive Population in Autoimmunity
    Article Snippet: Antibodies and reagents Anti-CD40 (sc-975) and anti-Bcl- XL/S (sc-634) were from Santa Cruz Biotechnology, Inc., Santa Cruz, California, and anti-cFLIP (F9800) from Sigma, St. Louis, MO. .. Biotinylated anti-CD40 antibodies (1C10 and 4F11) were produced in-house.

    Western Blot:

    Article Title: Modulation of SIV and HIV DNA Vaccine Immunity by Fas-FasL Signaling
    Article Snippet: Paragraph title: 2.2. Peptides, Antibodies, and Diagnostic Kits for Flow Cytometry and Western Blots ... Rabbit polyclonal antibodies for FADD (AV30294) and FLIP (F9800) were ordered from Sigma-Aldrich.

    Sonication:

    Article Title: PU.1 supports TRAIL-induced cell death by inhibiting NF-κB-mediated cell survival and inducing DR5 expression
    Article Snippet: The primary antibodies used were anti-PU.1 (no. 2258; Cell Signaling, Switzerland or sc-365208; Santa Cruz, Switzerland), anti-cFLIP (F9800; Sigma) and (AG-20B-0056; Adipogen AG, Switzerland), anti-XIAP (M044-3; MBL, Switzerland), anti-caspase-3 (235412; Calbiochem, Switzerland) and (no. 9661; Cell Signaling), anti-PARP (nos 9542, 9541; Cell Signaling) anti-cleaved caspase-8 (no. 9496; Cell Signaling), anti-caspase-8 (ALX-804-242; Enzo Life Sciences (ELS) AG, Switzerland), anti-Bcl-2 (SAB4500003; Sigma), anti-Mcl-1 (559027; BD Biosciences, Switzerland), anti-DR5 (no. 8074; Cell Signaling), anti-DcR2 (no. 8049; Cell Signaling), anti-p65 (no. 8242; Cell Signaling), anti-phospho-p65 (no. 3033; Cell Signaling), anti-I κ B α (no. 4814; Cell Signaling) anti-phospho-I κ B α (no. 2859; Cell Signaling), anti-RIPK1 (no. 3493; Cell Signaling) anti- α -tubulin (no. 3873; Cell Signaling, anti-Myc tag (ab13836; Abcam), anti-FLAG tag (no. 2368; Cell Signaling)) and anti-histone H3 (no. 9715; Cell Signaling). .. The lysates were briefly sonicated and then centrifuged at 13 × 105 × g for 15 min in a refrigerated microcentrifuge.

    Recombinant:

    Article Title: Modulation of SIV and HIV DNA Vaccine Immunity by Fas-FasL Signaling
    Article Snippet: Peptides, Antibodies, and Diagnostic Kits for Flow Cytometry and Western Blots The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: SIVmac 239 Gag (15-mer) Peptides-Complete Set, HIV-1 Consensus Subtype B Env (15-mer) Peptides-Complete Set, recombinant HIV-1BaL gp120, recombinant SIVmac251 Gag Pr55, HIV-1 gp120B sheep antiserum and mouse monoclonal antibody (mAb) to SIV p27. .. Rabbit polyclonal antibodies for FADD (AV30294) and FLIP (F9800) were ordered from Sigma-Aldrich.

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