anti e1a  (Millipore)


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    Name:
    Anti E1A Adenovirus Early Region 1A antibody
    Description:
    Adenovirus E1A gene modulates gene expression by associating with transcription factors in cells Studies have reported that E1A regulates the stimulation of ICAM 1 through inflammatory signals in pulmonary epithelial cells E1A has also been implicated in chronic obstructive pulmonary disease COPD Furthermore E1A is known to mediate tumor suppression by rendering epithelial cells sensitive to anoikis Sheep Anti E1A Adenovirus Early Region 1A antibody binds to E1A using immunoblotting 43kDa
    Catalog Number:
    a4345
    Price:
    None
    Applications:
    Anti-E1A (Adenovirus Early Region 1A) antibody can be used at 10mug/mL for western blot assays using whole extract of 293 cells.
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    Structured Review

    Millipore anti e1a
    Expression of E1 proteins . Western Blot analyses for <t>E1A</t> and 21-kDa E1B proteins expressed in 10 E1-transformed amniocyte cell pools. Cells were lysed and proteins were size fractionated on a SDS-containing polyacrylamide gel. Proteins were transferred to nitrocellulose and probed with anti-E1A or anti-E1B 21-kDa antibody. Protein lysates from HEK293 or primary amniocytes were used as control.
    Adenovirus E1A gene modulates gene expression by associating with transcription factors in cells Studies have reported that E1A regulates the stimulation of ICAM 1 through inflammatory signals in pulmonary epithelial cells E1A has also been implicated in chronic obstructive pulmonary disease COPD Furthermore E1A is known to mediate tumor suppression by rendering epithelial cells sensitive to anoikis Sheep Anti E1A Adenovirus Early Region 1A antibody binds to E1A using immunoblotting 43kDa
    https://www.bioz.com/result/anti e1a/product/Millipore
    Average 94 stars, based on 77 article reviews
    Price from $9.99 to $1999.99
    anti e1a - by Bioz Stars, 2021-01
    94/100 stars

    Images

    1) Product Images from "Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection"

    Article Title: Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-8-13

    Expression of E1 proteins . Western Blot analyses for E1A and 21-kDa E1B proteins expressed in 10 E1-transformed amniocyte cell pools. Cells were lysed and proteins were size fractionated on a SDS-containing polyacrylamide gel. Proteins were transferred to nitrocellulose and probed with anti-E1A or anti-E1B 21-kDa antibody. Protein lysates from HEK293 or primary amniocytes were used as control.
    Figure Legend Snippet: Expression of E1 proteins . Western Blot analyses for E1A and 21-kDa E1B proteins expressed in 10 E1-transformed amniocyte cell pools. Cells were lysed and proteins were size fractionated on a SDS-containing polyacrylamide gel. Proteins were transferred to nitrocellulose and probed with anti-E1A or anti-E1B 21-kDa antibody. Protein lysates from HEK293 or primary amniocytes were used as control.

    Techniques Used: Expressing, Western Blot, Transformation Assay

    Related Articles

    Transfection:

    Article Title: Role of the LXCXE Binding Site in Rb Function
    Article Snippet: .. For E1A binding, 5 μg of an E1a expression vector was transfected along with Rb expression vectors, and E1a was immunoprecipitated with an anti-E1a monoclonal antibody (Calbiochem). .. For colony formation assays , Saos-2 cells were grown to approximately 50% confluency on 100-mm-diameter plates and then cotransfected with 2 μg of an expression vector for the neomycin resistance gene and 20 μg of expression vector for wild-type or mutant Rb, using the calcium phosphate method.

    Immunoprecipitation:

    Article Title: Role of the LXCXE Binding Site in Rb Function
    Article Snippet: .. For E1A binding, 5 μg of an E1a expression vector was transfected along with Rb expression vectors, and E1a was immunoprecipitated with an anti-E1a monoclonal antibody (Calbiochem). .. For colony formation assays , Saos-2 cells were grown to approximately 50% confluency on 100-mm-diameter plates and then cotransfected with 2 μg of an expression vector for the neomycin resistance gene and 20 μg of expression vector for wild-type or mutant Rb, using the calcium phosphate method.

    Incubation:

    Article Title: Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection
    Article Snippet: .. The proteins were separated in a 10% SDS-polyacrylamid gel, transferred to nitrocellulose and incubated with either an anti-E1A or an anti-E1B-21kD antibody (Oncogene Research), and anti-mouse (E1A, Jackson ImmunoResearch Laboratories) or anti-rat (E1B-21kD, Oncogene Research) as secondary antibody and visualized by chemoluminescence. .. Expression of hAAT (Western Blot) The intracellular and secreted hAAT expression in different cell lines were analysed in a Western Blot using a monoclonal antibody.

    Article Title: Axl-Gas6 Interaction Counteracts E1A-Mediated Cell Growth Suppression and Proapoptotic Activity
    Article Snippet: .. The membranes were treated separately with an anti-Axl (Santa Cruz Biotech), an anti-E1A protein (Oncogene Research), or an anti-α-actin (Amersham Life Science) antibody and then incubated with peroxidase-conjugated secondary antibodies and detected by the enhanced-chemiluminescence method (Amersham Life Science). .. For the analysis of Gas6 expression, supernatants of cultures were subjected first to SDS-PAGE and then to immunoblotting with an anti-Gas6 polyclonal antibody (Santa Cruz Biotech).

    Expressing:

    Article Title: Role of the LXCXE Binding Site in Rb Function
    Article Snippet: .. For E1A binding, 5 μg of an E1a expression vector was transfected along with Rb expression vectors, and E1a was immunoprecipitated with an anti-E1a monoclonal antibody (Calbiochem). .. For colony formation assays , Saos-2 cells were grown to approximately 50% confluency on 100-mm-diameter plates and then cotransfected with 2 μg of an expression vector for the neomycin resistance gene and 20 μg of expression vector for wild-type or mutant Rb, using the calcium phosphate method.

    Western Blot:

    Article Title: Therapy of prostate cancer using a novel cancer terminator virus and a small molecule BH-3 mimetic
    Article Snippet: .. For Western blotting analyses, the primary antibodies used were mouse monoclonal anti-MDA-7/IL-24 (1:2000; Gen Hunter Corporation, Nashville, TN, USA), anti-E1A (1:1000; EMD Millipore), anti-EF1α (1:5000; EMD Millipore), anti-β-actin (1:5000; Sigma-Aldrich), rabbit monoclonal anti-Bcl-xL (1:1000), anti-PARP (1:1000), anti-Bcl-2 (1:1000), anti-Mcl-1, rabbit polyclonal anti-phospho p-38 and anti-p38 (1:1000; Cell Signaling Technology). .. The secondary antibodies used were polyclonal goat anti-mouse IgG (1:1000; Dako, Carpinteria, CA, USA) and polyclonal swine anti-rabbit IgG (1:3000; Dako).

    Article Title: DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells
    Article Snippet: .. Each lysate sample was subjected to 17% SDS-PAGE, and analyzed by Western blotting ( ) with the following primary antibodies: anti-MCL-1 rabbit polyclonal (Stressgen Biotechnologies); anti-BCL-2 hamster monoclonal polyclonal (PharMingen); anti-E1A and anti-actin mouse monoclonal (Oncogene Research); anti-E1B 19K rabbit polyclonal ( ); and anti-γH2AX (anti-H2AX phospho Ser 139) rabbit polyclonal (Upstate Biotechnology) antibodies. .. Blots were developed with horseradish peroxidase-conjugated secondary antibodies using the ECL system (Amersham-Pharmacia Biotech).

    Binding Assay:

    Article Title: Role of the LXCXE Binding Site in Rb Function
    Article Snippet: .. For E1A binding, 5 μg of an E1a expression vector was transfected along with Rb expression vectors, and E1a was immunoprecipitated with an anti-E1a monoclonal antibody (Calbiochem). .. For colony formation assays , Saos-2 cells were grown to approximately 50% confluency on 100-mm-diameter plates and then cotransfected with 2 μg of an expression vector for the neomycin resistance gene and 20 μg of expression vector for wild-type or mutant Rb, using the calcium phosphate method.

    SDS Page:

    Article Title: DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells
    Article Snippet: .. Each lysate sample was subjected to 17% SDS-PAGE, and analyzed by Western blotting ( ) with the following primary antibodies: anti-MCL-1 rabbit polyclonal (Stressgen Biotechnologies); anti-BCL-2 hamster monoclonal polyclonal (PharMingen); anti-E1A and anti-actin mouse monoclonal (Oncogene Research); anti-E1B 19K rabbit polyclonal ( ); and anti-γH2AX (anti-H2AX phospho Ser 139) rabbit polyclonal (Upstate Biotechnology) antibodies. .. Blots were developed with horseradish peroxidase-conjugated secondary antibodies using the ECL system (Amersham-Pharmacia Biotech).

    Plasmid Preparation:

    Article Title: Role of the LXCXE Binding Site in Rb Function
    Article Snippet: .. For E1A binding, 5 μg of an E1a expression vector was transfected along with Rb expression vectors, and E1a was immunoprecipitated with an anti-E1a monoclonal antibody (Calbiochem). .. For colony formation assays , Saos-2 cells were grown to approximately 50% confluency on 100-mm-diameter plates and then cotransfected with 2 μg of an expression vector for the neomycin resistance gene and 20 μg of expression vector for wild-type or mutant Rb, using the calcium phosphate method.

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