Structured Review

Santa Cruz Biotechnology mouse anti dynein
Working model of DIAPH3 function in aNPC. ( A ) During mitosis, DIAPH3 maintains the dynamics of cytoskeleton. Polarity proteins NUMA and <t>GPSM2</t> assemble underneath cortical F-actin and recruit the <t>dynein/dynactin</t> motor protein complex. Dynein and dynactin interact with SPAG5/KNSTRN/CLASP1 located at the microtubule plus-end and attach astral microtubules to cell cortex, thus providing the pulling force for chromosome bipolar segregation ( Okumura et al., 2018 , Dunsch et al., 2011 , Kern et al., 2016 ). ( B ) The position of polarity proteins NUMA/GPSM2 directs the orientation of the mitotic spindle and determines the type of division (proliferative versus neurogenic) of aNPC. ( C , D ) Absence of DIAPH3 destabilizes actin and microtubules and disrupts the expression of SPAG5, KNSTRN, GPSM2, PAR3 and NUMB, therefore weakening astral microtubules-cell cortex and spindle microtubules-kinetochore interactions. This causes spindle abnormalities, chromosome mis-alignment and mis-segregation, and alters fate decision of aNPC.
Mouse Anti Dynein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 3 article reviews
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mouse anti dynein - by Bioz Stars, 2022-11
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1) Product Images from "DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction"

Article Title: DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction

Journal: bioRxiv

doi: 10.1101/2020.08.11.245829

Working model of DIAPH3 function in aNPC. ( A ) During mitosis, DIAPH3 maintains the dynamics of cytoskeleton. Polarity proteins NUMA and GPSM2 assemble underneath cortical F-actin and recruit the dynein/dynactin motor protein complex. Dynein and dynactin interact with SPAG5/KNSTRN/CLASP1 located at the microtubule plus-end and attach astral microtubules to cell cortex, thus providing the pulling force for chromosome bipolar segregation ( Okumura et al., 2018 , Dunsch et al., 2011 , Kern et al., 2016 ). ( B ) The position of polarity proteins NUMA/GPSM2 directs the orientation of the mitotic spindle and determines the type of division (proliferative versus neurogenic) of aNPC. ( C , D ) Absence of DIAPH3 destabilizes actin and microtubules and disrupts the expression of SPAG5, KNSTRN, GPSM2, PAR3 and NUMB, therefore weakening astral microtubules-cell cortex and spindle microtubules-kinetochore interactions. This causes spindle abnormalities, chromosome mis-alignment and mis-segregation, and alters fate decision of aNPC.
Figure Legend Snippet: Working model of DIAPH3 function in aNPC. ( A ) During mitosis, DIAPH3 maintains the dynamics of cytoskeleton. Polarity proteins NUMA and GPSM2 assemble underneath cortical F-actin and recruit the dynein/dynactin motor protein complex. Dynein and dynactin interact with SPAG5/KNSTRN/CLASP1 located at the microtubule plus-end and attach astral microtubules to cell cortex, thus providing the pulling force for chromosome bipolar segregation ( Okumura et al., 2018 , Dunsch et al., 2011 , Kern et al., 2016 ). ( B ) The position of polarity proteins NUMA/GPSM2 directs the orientation of the mitotic spindle and determines the type of division (proliferative versus neurogenic) of aNPC. ( C , D ) Absence of DIAPH3 destabilizes actin and microtubules and disrupts the expression of SPAG5, KNSTRN, GPSM2, PAR3 and NUMB, therefore weakening astral microtubules-cell cortex and spindle microtubules-kinetochore interactions. This causes spindle abnormalities, chromosome mis-alignment and mis-segregation, and alters fate decision of aNPC.

Techniques Used: Expressing

Diaph3-deficiency impairs the expression and stability of mitotic spindle polarity proteins. ( A, B ) Assessment of NUMA, GPSM2, INSC, NUMB, PAR3, Dynein, Dynactin, SPAG5, KNSTRN, and CLASP1 levels in telencephalon extracts of Diaph3 KO mice by western blotting. CENPA was used as positive control ( Liu and Mao, 2016 ) and GAPDH as loading control for quantification ( n =3 embryos for each genotype. *: P
Figure Legend Snippet: Diaph3-deficiency impairs the expression and stability of mitotic spindle polarity proteins. ( A, B ) Assessment of NUMA, GPSM2, INSC, NUMB, PAR3, Dynein, Dynactin, SPAG5, KNSTRN, and CLASP1 levels in telencephalon extracts of Diaph3 KO mice by western blotting. CENPA was used as positive control ( Liu and Mao, 2016 ) and GAPDH as loading control for quantification ( n =3 embryos for each genotype. *: P

Techniques Used: Expressing, Mouse Assay, Western Blot, Positive Control

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    Santa Cruz Biotechnology dynein ic1 2 cytosolic antibody
    Dynein Ic1 2 Cytosolic Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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