Review



anti ddx21 rabbit polyclonal ab  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Proteintech anti ddx21 rabbit polyclonal ab
    Protein–protein interaction between DDX1 and <t>DDX21</t> was impeded by RTCB. (A) HeLa cells were cotransfected with Myc-RTCB and HA-DDX1. The localization of RTCB (green) and DDX1 (red) was assessed using confocal microscopy. DAPI was used to stain the nuclei (blue). (B) A549 cells from three 10-cm dishes were lysed with 500 μl IP lysis buffer, and immunoprecipitation was performed using anti-RTCB, anti-DDX1, or control IgG Abs. The pull-down products were analyzed using Western blotting. (C) HEK293T cells were cotransfected with Myc-RTCB and HA-DDX1. The co-IP experiment was performed using an anti-Myc or an anti-HA Ab, followed by Western blotting. (D and E) RTCBWT and RTCBKO A549 cells were cultured in 10-cm dishes and lysed with 500 μl IP lysis buffer, followed by immunoprecipitation assay with anti-DDX1, anti-DDX21, or control IgG; the pull-down products were analyzed using Western blotting. (F and G) HEK293T cells were cotransfected with HA-DDX1, Flag-DDX21, Myc-DHX36, or an empty vector, as well as with increasing quantities of Myc-RTCB for 24 h. The co-IP experiment was performed using an anti-HA or an anti-Flag Ab, followed by Western blotting. In each Western blot assay, the blots originated either from the same membrane or from reloading the same quantity of lysates in the same experiments. (H) The proposed model of the action of RTCB on the conformation of the DDX1-DDX21-DDX36 complex.
    Anti Ddx21 Rabbit Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ddx21 rabbit polyclonal ab/product/Proteintech
    Average 93 stars, based on 22 article reviews
    anti ddx21 rabbit polyclonal ab - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1"

    Article Title: The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.2200799

    Protein–protein interaction between DDX1 and DDX21 was impeded by RTCB. (A) HeLa cells were cotransfected with Myc-RTCB and HA-DDX1. The localization of RTCB (green) and DDX1 (red) was assessed using confocal microscopy. DAPI was used to stain the nuclei (blue). (B) A549 cells from three 10-cm dishes were lysed with 500 μl IP lysis buffer, and immunoprecipitation was performed using anti-RTCB, anti-DDX1, or control IgG Abs. The pull-down products were analyzed using Western blotting. (C) HEK293T cells were cotransfected with Myc-RTCB and HA-DDX1. The co-IP experiment was performed using an anti-Myc or an anti-HA Ab, followed by Western blotting. (D and E) RTCBWT and RTCBKO A549 cells were cultured in 10-cm dishes and lysed with 500 μl IP lysis buffer, followed by immunoprecipitation assay with anti-DDX1, anti-DDX21, or control IgG; the pull-down products were analyzed using Western blotting. (F and G) HEK293T cells were cotransfected with HA-DDX1, Flag-DDX21, Myc-DHX36, or an empty vector, as well as with increasing quantities of Myc-RTCB for 24 h. The co-IP experiment was performed using an anti-HA or an anti-Flag Ab, followed by Western blotting. In each Western blot assay, the blots originated either from the same membrane or from reloading the same quantity of lysates in the same experiments. (H) The proposed model of the action of RTCB on the conformation of the DDX1-DDX21-DDX36 complex.
    Figure Legend Snippet: Protein–protein interaction between DDX1 and DDX21 was impeded by RTCB. (A) HeLa cells were cotransfected with Myc-RTCB and HA-DDX1. The localization of RTCB (green) and DDX1 (red) was assessed using confocal microscopy. DAPI was used to stain the nuclei (blue). (B) A549 cells from three 10-cm dishes were lysed with 500 μl IP lysis buffer, and immunoprecipitation was performed using anti-RTCB, anti-DDX1, or control IgG Abs. The pull-down products were analyzed using Western blotting. (C) HEK293T cells were cotransfected with Myc-RTCB and HA-DDX1. The co-IP experiment was performed using an anti-Myc or an anti-HA Ab, followed by Western blotting. (D and E) RTCBWT and RTCBKO A549 cells were cultured in 10-cm dishes and lysed with 500 μl IP lysis buffer, followed by immunoprecipitation assay with anti-DDX1, anti-DDX21, or control IgG; the pull-down products were analyzed using Western blotting. (F and G) HEK293T cells were cotransfected with HA-DDX1, Flag-DDX21, Myc-DHX36, or an empty vector, as well as with increasing quantities of Myc-RTCB for 24 h. The co-IP experiment was performed using an anti-HA or an anti-Flag Ab, followed by Western blotting. In each Western blot assay, the blots originated either from the same membrane or from reloading the same quantity of lysates in the same experiments. (H) The proposed model of the action of RTCB on the conformation of the DDX1-DDX21-DDX36 complex.

    Techniques Used: Confocal Microscopy, Staining, Lysis, Immunoprecipitation, Control, Western Blot, Co-Immunoprecipitation Assay, Cell Culture, Plasmid Preparation, Membrane



    Similar Products

    anti ddx21 rabbit polyclonal ab  (Proteintech)
    93
    Proteintech anti ddx21 rabbit polyclonal ab
    Protein–protein interaction between DDX1 and <t>DDX21</t> was impeded by RTCB. (A) HeLa cells were cotransfected with Myc-RTCB and HA-DDX1. The localization of RTCB (green) and DDX1 (red) was assessed using confocal microscopy. DAPI was used to stain the nuclei (blue). (B) A549 cells from three 10-cm dishes were lysed with 500 μl IP lysis buffer, and immunoprecipitation was performed using anti-RTCB, anti-DDX1, or control IgG Abs. The pull-down products were analyzed using Western blotting. (C) HEK293T cells were cotransfected with Myc-RTCB and HA-DDX1. The co-IP experiment was performed using an anti-Myc or an anti-HA Ab, followed by Western blotting. (D and E) RTCBWT and RTCBKO A549 cells were cultured in 10-cm dishes and lysed with 500 μl IP lysis buffer, followed by immunoprecipitation assay with anti-DDX1, anti-DDX21, or control IgG; the pull-down products were analyzed using Western blotting. (F and G) HEK293T cells were cotransfected with HA-DDX1, Flag-DDX21, Myc-DHX36, or an empty vector, as well as with increasing quantities of Myc-RTCB for 24 h. The co-IP experiment was performed using an anti-HA or an anti-Flag Ab, followed by Western blotting. In each Western blot assay, the blots originated either from the same membrane or from reloading the same quantity of lysates in the same experiments. (H) The proposed model of the action of RTCB on the conformation of the DDX1-DDX21-DDX36 complex.
    Anti Ddx21 Rabbit Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ddx21 rabbit polyclonal ab/product/Proteintech
    Average 93 stars, based on 1 article reviews
    anti ddx21 rabbit polyclonal ab - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Protein–protein interaction between DDX1 and DDX21 was impeded by RTCB. (A) HeLa cells were cotransfected with Myc-RTCB and HA-DDX1. The localization of RTCB (green) and DDX1 (red) was assessed using confocal microscopy. DAPI was used to stain the nuclei (blue). (B) A549 cells from three 10-cm dishes were lysed with 500 μl IP lysis buffer, and immunoprecipitation was performed using anti-RTCB, anti-DDX1, or control IgG Abs. The pull-down products were analyzed using Western blotting. (C) HEK293T cells were cotransfected with Myc-RTCB and HA-DDX1. The co-IP experiment was performed using an anti-Myc or an anti-HA Ab, followed by Western blotting. (D and E) RTCBWT and RTCBKO A549 cells were cultured in 10-cm dishes and lysed with 500 μl IP lysis buffer, followed by immunoprecipitation assay with anti-DDX1, anti-DDX21, or control IgG; the pull-down products were analyzed using Western blotting. (F and G) HEK293T cells were cotransfected with HA-DDX1, Flag-DDX21, Myc-DHX36, or an empty vector, as well as with increasing quantities of Myc-RTCB for 24 h. The co-IP experiment was performed using an anti-HA or an anti-Flag Ab, followed by Western blotting. In each Western blot assay, the blots originated either from the same membrane or from reloading the same quantity of lysates in the same experiments. (H) The proposed model of the action of RTCB on the conformation of the DDX1-DDX21-DDX36 complex.

    Journal: The Journal of Immunology Author Choice

    Article Title: The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1

    doi: 10.4049/jimmunol.2200799

    Figure Lengend Snippet: Protein–protein interaction between DDX1 and DDX21 was impeded by RTCB. (A) HeLa cells were cotransfected with Myc-RTCB and HA-DDX1. The localization of RTCB (green) and DDX1 (red) was assessed using confocal microscopy. DAPI was used to stain the nuclei (blue). (B) A549 cells from three 10-cm dishes were lysed with 500 μl IP lysis buffer, and immunoprecipitation was performed using anti-RTCB, anti-DDX1, or control IgG Abs. The pull-down products were analyzed using Western blotting. (C) HEK293T cells were cotransfected with Myc-RTCB and HA-DDX1. The co-IP experiment was performed using an anti-Myc or an anti-HA Ab, followed by Western blotting. (D and E) RTCBWT and RTCBKO A549 cells were cultured in 10-cm dishes and lysed with 500 μl IP lysis buffer, followed by immunoprecipitation assay with anti-DDX1, anti-DDX21, or control IgG; the pull-down products were analyzed using Western blotting. (F and G) HEK293T cells were cotransfected with HA-DDX1, Flag-DDX21, Myc-DHX36, or an empty vector, as well as with increasing quantities of Myc-RTCB for 24 h. The co-IP experiment was performed using an anti-HA or an anti-Flag Ab, followed by Western blotting. In each Western blot assay, the blots originated either from the same membrane or from reloading the same quantity of lysates in the same experiments. (H) The proposed model of the action of RTCB on the conformation of the DDX1-DDX21-DDX36 complex.

    Article Snippet: Abs The Abs used in this study were anti-RTCB rabbit polyclonal Ab (Cat no. 19809-1-AP; Proteintech), anti-DDX1 rabbit polyclonal Ab (Cat no. 11357-1-AP; Proteintech), anti-DDX21 rabbit polyclonal Ab (Cat no. 10528-1-AP; Proteintech), anti-DHX36 rabbit polyclonal Ab (Cat no. 13159-1-AP; Proteintech), anti-Flag mouse mAb (Cat no. F1804; Sigma), anti-HA rabbit polyclonal Ab (Cat no. 51064-2-AP; Proteintech), anti-GAPDH mouse mAb (Cat no. 60004-1-Ig; Proteintech), as well as Alexa Fluor 488–conjugated AffiniPure goat anti-mouse (Cat no. GM200G-02C; Sungene Biotech, Tianjin, China) and Alexa Fluor 594–conjugated AffiniPure goat anti-rabbit (Cat no. GR200G-43C; Sungene Biotech) secondary Abs.

    Techniques: Confocal Microscopy, Staining, Lysis, Immunoprecipitation, Control, Western Blot, Co-Immunoprecipitation Assay, Cell Culture, Plasmid Preparation, Membrane