Journal: Oncology Letters

Article Title: Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review

doi: 10.3892/ol.2024.14601

Figure Lengend Snippet: Right inguinal lymph node biopsy with an (A) hematoxylin and eosin-stained section (magnification, 100×) showing partial disappearance of the normal structure. Tumor cells (magnification, 400×) (B) CD3-diffusely bright positive, (C) CD5-positive, (D) CD43-positive, (E) CD99-positive, (F) terminal deoxynucleotidyl transferase partially positive, (G) CD34-negative, (H) diffusely bright Ki-67-positive (+70%), (I) CD20-negative, (J) CD21-negative, (K) CD56-negative, (L) myeloperoxidase-negative, (M) CD68-(loose positive), (N) Bcl-2-negative, (O) Bcl-6-negative, (P) CD10-negative, (Q) CD117-negative, (R) CD15-negative, (S) cyclinD1-negative, (T) MUM-1-negative, (U) PAX-5-negative, (V) EBER-negative, (W) CD1A-positive, (X) CD4-positive and (Y) CD8-negative.

Article Snippet: The following primary antibodies were added: Anti-CD1A (cat. no. ab313875; 0.1 µg/ml; Abcam), anti-CD4 (cat. no. ab133616; 0.2 µg/ml; Abcam), anti-CD8 (cat. no. ab245118; 0.4 µg/ml; Abcam), anti-CD3 (cat. no. ab243874; 0.2 µg/ml; Abcam), anti-CD43 (cat. no. ab101533; 0.5 µg/ml; Abcam), anti-CD5 (cat. no. ab75877; 0.2 µg/ml; Abcam), anti-CD68 (cat. no. ab213363; 0.08 µg/ml; Abcam), anti-CD99 (cat. no. ab108297; 0.5 µg/ml; Abcam), anti-terminal deucleotide transferase (cat. no. ab183341; 1 ug/ml; Abcam), anti-Ki-67 (cat. no. ab92742; 1 µg/ml; Abcam), anti-Bcl-2 (cat. no. ab32124; 1 µg/ml; Abcam), anti-Bcl-6 (cat. no. ab172610; 0.5 µg/ml; Abcam), anti-CD10 (cat. no. ab208778; 0.1 µg/ml; Abcam), anti-CD117 (cat. no. ab32363; 1 µg/ml; Abcam), anti-CD15 (cat. no. ab218403; 5 µg/ml; Abcam), anti-CD20 (cat. no. ab219329; 1 µg/ml; Abcam), anti-CD21 (cat. no. ab315160; 2 µg/ml; Abcam), anti-CD34 (cat. no. ab315820; 0.1 µg/ml; Abcam), anti-CD56 (cat. no. ab313779; 0.2 µg/ml; Abcam), anti-cyclind1 (cat. no. ab273608; 1 µg/ml; Abcam), anti-myeloperoxidase (cat. no. ab134142; 0.1 µg/ml; Abcam), anti-mutated melanoma-associated antigen 1 (cat. no. ab247079; 0.5 µg/ml; Abcam), anti-pin-box protein Pax-5 (cat. no. ab234402; 0.5 µg/ml; Abcam) and incubated at 3°C for 1–2 h or 4°C overnight.

Techniques: Staining