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Effect of OMT on the expression of proteins associated with proliferation and apoptosis. Western blot was used to detect the protein expression of <t>Cyclin</t> <t>D1,</t> Bcl-2, Bax and Cleaved caspase-3. Gray value analysis of the target protein relative to β-actin. Data were expressed as mean ± standard error, P < 0.05, P < 0.01
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Effect of OMT on the expression of proteins associated with proliferation and apoptosis. Western blot was used to detect the protein expression of Cyclin D1, Bcl-2, Bax and Cleaved caspase-3. Gray value analysis of the target protein relative to β-actin. Data were expressed as mean ± standard error, P < 0.05, P < 0.01

Journal: Discover Oncology

Article Title: Inhibition of hemangioma development by regulating the VEGF/VEGFR autocrine loop via the miR-494/PTEN pathway

doi: 10.1007/s12672-025-01802-1

Figure Lengend Snippet: Effect of OMT on the expression of proteins associated with proliferation and apoptosis. Western blot was used to detect the protein expression of Cyclin D1, Bcl-2, Bax and Cleaved caspase-3. Gray value analysis of the target protein relative to β-actin. Data were expressed as mean ± standard error, P < 0.05, P < 0.01

Article Snippet: The cells were blocked with 5% skim milk powder for 1 h, followed by the addition of primary antibodies Cyclin D1 (Catalog No. 2926, 1:1000), Bcl-2 (Catalog No. 2870, 1:1000), Bax (Catalog No. 2772, 1:1000), Cleaved Caspase-3 (Catalog No. 9661, 1:1000), PTEN (Catalog No. 9188, 1:1000), VEGFR-2 (Catalog No. 2479, 1:1000), p-VEGFR-2 (Catalog No. 2478, 1:1000), ERK (Catalog No. 9102, 1:1000), p-ERK (Catalog No. 4370, 1:1000), and β-actin (Catalog No. 4970, 1:1000), purchased from Cell Signaling Technology) and incubated overnight at 4° C. The corresponding HRP-labeled secondary antibody was added and incubated for 1 h at room temperature.

Techniques: Expressing, Western Blot