Journal: Frontiers in Cell and Developmental Biology

Article Title: Genome-wide analysis reveals the MORC3-mediated repression of PD-L1 expression in head and neck cancer

doi: 10.3389/fcell.2024.1410130

Figure Lengend Snippet: MORC3 plays negative roles in cancer cell proliferation. (A) CAL 27 cells were transfected with anti-MORC3 or non-specific (NS) siRNA, and cell numbers were counted on Day 2 and Day 4. (B, C) The expression levels of CDH1, KRT14, JUN, CCND1, CCND2, CCN1, and IL6 in CAL 27 cells with or without MORC3 knockdown were analyzed by qRT-PCR. *, p < 0.05.

Article Snippet: The membranes were blocked with 5% skimmed milk and incubated overnight at 4°C with following antibodies: mouse monoclonal anti-MORC3 antibody (Santa Cruz Biotechnology, United States), rabbit monoclonal anti- interferon induced protein with tetratricopeptide repeats 2 (IFIT2) antibody (abcam, United States), rabbit monoclonal anti-interferon induced transmembrane protein 3 (IFITM3) antibody (Cell Signaling Technology, United States), rabbit monoclonal anti-interferon induced protein with tetratricopeptide repeats 1 (IFIT1) antibody (Cell Signaling Technology, United States), rabbit monoclonal anti-cyclin D1 (CCND1) (ABclonal, China), rabbit monoclonal anti-cyclinD2 (CCND2) (ABclonal, China), rabbit polyclonal anti-JUN (ABclonal, China), rabbit polyclonal anti-interferon regulatory factor 7 (IRF7) (Proteintech, China), rabbit polyclonal anti-PD-L1 antibody (Proteintech, China), rabbit polyclonal anti-DExD/H-Box Helicase 60 (DDX60) antibody (Proteintech, China), and mouse anti-β-actin (Abmart, China).

Techniques: Transfection, Expressing, Knockdown, Quantitative RT-PCR

Journal: Journal of Translational Medicine

Article Title: The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia

doi: 10.1186/s12967-024-05523-x

Figure Lengend Snippet: The primer sequence of the target genes

Article Snippet: Cyclin D1 (CCND1) , 1:1000 , Bioss, China.

Techniques: Sequencing

Journal: Journal of Translational Medicine

Article Title: The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia

doi: 10.1186/s12967-024-05523-x

Figure Lengend Snippet: List of Western blotting primary antibody

Article Snippet: Cyclin D1 (CCND1) , 1:1000 , Bioss, China.

Techniques: Western Blot

Journal: Journal of Translational Medicine

Article Title: The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia

doi: 10.1186/s12967-024-05523-x

Figure Lengend Snippet: List of Immunofluorescence staining primary antibody

Article Snippet: Cyclin D1 (CCND1) , 1:1000 , Bioss, China.

Techniques: Immunofluorescence, Staining

Journal: Journal of Translational Medicine

Article Title: The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia

doi: 10.1186/s12967-024-05523-x

Figure Lengend Snippet: Scleral cell cycle measurement and apoptotic analysis. A , B Measurement of CDK2 and CCND1 expression at the mRNA level in the sclera of the guinea pigs in NC and LIM groups (n = 4–6). C CDK2 and CCND1 immunoblots. D , E Measurement of CDK2 and CCND1 expression at protein level by Western blot in the sclera of the guinea pigs in NC and LIM groups (n = 6). F Measurement of CDK2 and CCND1 expression at protein level by immunofluorescence in the sclera of the guinea pigs in NC and LIM groups. (Western Blot cropped) (n = 6)

Article Snippet: Cyclin D1 (CCND1) , 1:1000 , Bioss, China.

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: PeerJ

Article Title: Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions

doi: 10.7717/peerj.17806

Figure Lengend Snippet: (A) Validation of the differentially expressed genes by q-PCR with eight replicates. (B and C) Western blot analysis of the expression of proteins related to cell proliferation. (D and E) Cell immunofluorescence detection of the protein levels of c-Jun and CCND1. Three biological replicates were performed for Western blot assay. The p values of the comparison between control group and Halo-rhEGF group are all less than 0.05. Scale bar: 200 μm.

Article Snippet: The membrane was blocked with 5% (w/v) skimmed milk in TBST (20 mM Tris, 150 mM NaCl and 0.1% Tween in water, pH 7.5) and incubated with primary antibodies against ERK1/2, phos-ERK1/2, c-Jun, phos-c-Jun, Tubulin, Cyclin D1 (CCND1) at 4 °C for 24 h. The membranes were incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and the labelled protein bands were visualized on Amersham Imager 600 (Amersham Biosciences, Piscataway, NJ, USA).

Techniques: Western Blot, Expressing, Immunofluorescence, Comparison, Control

Journal: Nagoya Journal of Medical Science

Article Title: Protective effect of Sasa veitchii extract against all-trans-retinoic acid-induced inhibition of proliferation of cultured human palate cells

doi: 10.18999/nagjms.86.2.223

Figure Lengend Snippet: Involvement with apoptosis and cell cycle by treatment with of SE against at RA in HEPM cells Fig. 3A: Apotracker staining (green) of HEPM cells after treatment with 100 μM at RA and 0.03% SE for 48 h. The nuclei were counterstained with Hoechst 33342 (blue). Scale bar, 50 μm. Fig. 3B: Immunoblotting of HEPM cells after treatment with 100 μM at RA and 0.03% SE for 48 h. β-actin was served as an internal control. *p < 0.05 and ***p < 0.001 versus at RA. at RA: all-trans -retinoic acid HEPM: human embryonic palatal mesenchymal SE: Sasa veitchii extract CCND1: cyclin D1

Article Snippet: Rabbit anti-cleaved caspase-3 polyclonal antibody (1:2,500 dilution; Cell Signaling Technology, Beverly, MA), mouse anti-cyclin D1 (CCND1) monoclonal antibody (1:1,000 dilution; Santa Cruz Biotechnology, Dallas, TX), mouse anti-CCNA (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNB (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNE (1:1,000 dilution; Santa Cruz Biotechnology), and anti-mouse β-actin monoclonal antibody (1:2,500 dilution; MBL, Aichi, Japan) were used as primary antibodies for immunoblotting.

Techniques: Staining, Western Blot, Control

Journal: Nagoya Journal of Medical Science

Article Title: Protective effect of Sasa veitchii extract against all-trans-retinoic acid-induced inhibition of proliferation of cultured human palate cells

doi: 10.18999/nagjms.86.2.223

Figure Lengend Snippet: Proposed mechanism of SE against at RA-induced cell proliferation inhibition ERBB2: Erb-B2 receptor tyrosine kinase 2 JADE1 : jade family PHD finger 1 CCND1: cyclin D1

Article Snippet: Rabbit anti-cleaved caspase-3 polyclonal antibody (1:2,500 dilution; Cell Signaling Technology, Beverly, MA), mouse anti-cyclin D1 (CCND1) monoclonal antibody (1:1,000 dilution; Santa Cruz Biotechnology, Dallas, TX), mouse anti-CCNA (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNB (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNE (1:1,000 dilution; Santa Cruz Biotechnology), and anti-mouse β-actin monoclonal antibody (1:2,500 dilution; MBL, Aichi, Japan) were used as primary antibodies for immunoblotting.

Techniques: Inhibition