anti cleaved caspase 3  (R&D Systems)

 
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    Name:
    Human Mouse Active Caspase 3 Antibody
    Description:
    The Human Mouse Active Caspase 3 Antibody from R D Systems is a rabbit polyclonal antibody to Caspase 3 This antibody reacts with human mouse The Human Mouse Active Caspase 3 Antibody has been validated for the following applications Immunocytochemistry
    Catalog Number:
    af835
    Price:
    299
    Applications:
    Immunocytochemistry
    Host:
    Rabbit
    Purity:
    Immunogen affinity purified
    Conjugate:
    Unconjugated
    Immunogen:
    KLH-coupled human Caspase-3 synthetic peptide, CRGTELDCGIETD, Accession # P42574
    Size:
    50 ug
    Category:
    Primary Antibodies
    Isotype:
    IgG
    Buy from Supplier


    Structured Review

    R&D Systems anti cleaved caspase 3
    Human Mouse Active Caspase 3 Antibody
    The Human Mouse Active Caspase 3 Antibody from R D Systems is a rabbit polyclonal antibody to Caspase 3 This antibody reacts with human mouse The Human Mouse Active Caspase 3 Antibody has been validated for the following applications Immunocytochemistry
    https://www.bioz.com/result/anti cleaved caspase 3/product/R&D Systems
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2020-09
    95/100 stars

    Images

    1) Product Images from "Bcl-2 is a critical mediator of intestinal transformation"

    Article Title: Bcl-2 is a critical mediator of intestinal transformation

    Journal: Nature Communications

    doi: 10.1038/ncomms10916

    Bcl-2 is dispensable for ISCs during regeneration. ( a ) Panel of sections stained for cleaved caspase-3 (CC-3) in Lgr5.Bcl-2 +/+ mice (top) and Lgr5.Bcl-2 fl/fl mice (bottom) 5 weeks after tamoxifen induction and 4 h after 6 Gy full-body irradiation. Scale bars, 100 μm. ( b ) Graph displaying the percentage of CC-3 foci per crypt for Lgr5 . Bcl-2 +/+ and Lgr5 . Bcl-2 fl/fl mice at the indicated time points post 6 Gy irradiation. A minimum of 80 crypts was counted for each genotype and time point. Error bars represent the s.e.m., * P
    Figure Legend Snippet: Bcl-2 is dispensable for ISCs during regeneration. ( a ) Panel of sections stained for cleaved caspase-3 (CC-3) in Lgr5.Bcl-2 +/+ mice (top) and Lgr5.Bcl-2 fl/fl mice (bottom) 5 weeks after tamoxifen induction and 4 h after 6 Gy full-body irradiation. Scale bars, 100 μm. ( b ) Graph displaying the percentage of CC-3 foci per crypt for Lgr5 . Bcl-2 +/+ and Lgr5 . Bcl-2 fl/fl mice at the indicated time points post 6 Gy irradiation. A minimum of 80 crypts was counted for each genotype and time point. Error bars represent the s.e.m., * P

    Techniques Used: Staining, Mouse Assay, Irradiation

    2) Product Images from "Lesion stage-dependent causes for impaired remyelination in MS"

    Article Title: Lesion stage-dependent causes for impaired remyelination in MS

    Journal: Acta Neuropathologica

    doi: 10.1007/s00401-020-02189-9

    Supernatants of primary human M1, but not M2 or M0 polarized microglia inhibit the terminal differentiation of hiOL. Every experiment analyzing the effect of microglia supernatants on hiOL was performed with hiOL derived from three different donors. a – e To confirm successful polarization into M1 and M2 microglia, qRT-PCR was performed for IL-6, CXCL10 and TNFα (M1) and CD206 and CD209 (M2). f Differentiation of hiOL in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 4 to 21 resulted in no significant differences in the percentage of O4 + hiOL when comparing the effect of different supernatants with appropriate controls. g , h Culturing O4 sorted hiOL in the presence of supernatants from M1, but not M0 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 impaired significantly the differentiation of O4 + hiOL into MBP + mature oligodendrocytes. i – k This was confirmed using supernatants from M1 and M0 polarized microglia from another fetal (HFM1) and one adult donor (HAM1) as well as supernatants from M0, M1 and M2 polarized microglia from a second adult donor (HAM2). l Analysis of percentages of actively dividing hiOL (Ki-67 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 21 to 35 of differentiation did not reveal any significant differences. m Percentages of apoptotic hiOL (cleaved caspase 3 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 of differentiation were not significantly altered. Scale bar in g : 100 µm. hiOL human iPSC-derived oligodendrocytes, HFM human fetal microglia, HAM human adult microglia, IL-6 interleukin 6, CXCL10 C-X-C motif chemokine 10, TNFα tumor necrosis factor alpha, CD206/CD209 cluster of differentiation 206/209, MBP myelin basic protein
    Figure Legend Snippet: Supernatants of primary human M1, but not M2 or M0 polarized microglia inhibit the terminal differentiation of hiOL. Every experiment analyzing the effect of microglia supernatants on hiOL was performed with hiOL derived from three different donors. a – e To confirm successful polarization into M1 and M2 microglia, qRT-PCR was performed for IL-6, CXCL10 and TNFα (M1) and CD206 and CD209 (M2). f Differentiation of hiOL in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 4 to 21 resulted in no significant differences in the percentage of O4 + hiOL when comparing the effect of different supernatants with appropriate controls. g , h Culturing O4 sorted hiOL in the presence of supernatants from M1, but not M0 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 impaired significantly the differentiation of O4 + hiOL into MBP + mature oligodendrocytes. i – k This was confirmed using supernatants from M1 and M0 polarized microglia from another fetal (HFM1) and one adult donor (HAM1) as well as supernatants from M0, M1 and M2 polarized microglia from a second adult donor (HAM2). l Analysis of percentages of actively dividing hiOL (Ki-67 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 21 to 35 of differentiation did not reveal any significant differences. m Percentages of apoptotic hiOL (cleaved caspase 3 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 of differentiation were not significantly altered. Scale bar in g : 100 µm. hiOL human iPSC-derived oligodendrocytes, HFM human fetal microglia, HAM human adult microglia, IL-6 interleukin 6, CXCL10 C-X-C motif chemokine 10, TNFα tumor necrosis factor alpha, CD206/CD209 cluster of differentiation 206/209, MBP myelin basic protein

    Techniques Used: Derivative Assay, Quantitative RT-PCR

    3) Product Images from "Tbx1 and Brn4 regulate retinoic acid metabolic genes during cochlear morphogenesis"

    Article Title: Tbx1 and Brn4 regulate retinoic acid metabolic genes during cochlear morphogenesis

    Journal: BMC Developmental Biology

    doi: 10.1186/1471-213X-9-31

    TCre-KO embryos exhibit defects in cell proliferation and survival . Transverse sections at E11.5 of control (A) and TCre-KO (C) embryos labeled for mitotic cells using an Ab against phospho-Histone 3. TCre-KO embryos display a decrease in cell proliferation in the otic epithelium compared to controls upon quantification (E). Proliferation was reduced in both dorsal and ventral regions of the otic vesicle, but not in the POM. For the dorsal otic vesicle, 11.2 and 21.4 positive cells were observed for mutant and control, respectively, while 14.0 and 22.4 positive cells were observed observed for the ventral otic vesicle. TCre-KO embryos exhibit an increase in apoptosis at E11.5, identified by an Ab to active Caspase 3 (B, D). Arrow and bracketed area in (d) mark apoptotic cells. Quantification of apoptotic cells in control and mutant otic epithelium is shown (F). Asterisks indicate significant difference (* = p
    Figure Legend Snippet: TCre-KO embryos exhibit defects in cell proliferation and survival . Transverse sections at E11.5 of control (A) and TCre-KO (C) embryos labeled for mitotic cells using an Ab against phospho-Histone 3. TCre-KO embryos display a decrease in cell proliferation in the otic epithelium compared to controls upon quantification (E). Proliferation was reduced in both dorsal and ventral regions of the otic vesicle, but not in the POM. For the dorsal otic vesicle, 11.2 and 21.4 positive cells were observed for mutant and control, respectively, while 14.0 and 22.4 positive cells were observed observed for the ventral otic vesicle. TCre-KO embryos exhibit an increase in apoptosis at E11.5, identified by an Ab to active Caspase 3 (B, D). Arrow and bracketed area in (d) mark apoptotic cells. Quantification of apoptotic cells in control and mutant otic epithelium is shown (F). Asterisks indicate significant difference (* = p

    Techniques Used: Labeling, Mutagenesis

    4) Product Images from "Targeting NF-κB Signaling by Calebin A, a Compound of Turmeric, in Multicellular Tumor Microenvironment: Potential Role of Apoptosis Induction in CRC Cells"

    Article Title: Targeting NF-κB Signaling by Calebin A, a Compound of Turmeric, in Multicellular Tumor Microenvironment: Potential Role of Apoptosis Induction in CRC Cells

    Journal: Biomedicines

    doi: 10.3390/biomedicines8080236

    Effect of Calebin A (CA) or specific IKK inhibitor BMS-345541 on activation of NF-κB and NF-κB-regulated gene end-products in CRC cells: Serum-starved HCT116 cells in alginate cultures from multicellular pro-inflammatory TME cultures were treated as described in Materials and Methods. Immunoblotting of whole-cell lysates from HCT116 was performed for anti-p65-NF-κB, anti-phospho-p65-NF-κB, anti-MMP-9, anti-CXCR4, anti-Cyclin D1, and anti-cleaved-caspase-3. β-actin served as an internal loading control in all experiments. For densitometric evaluation, results are compared to control and p
    Figure Legend Snippet: Effect of Calebin A (CA) or specific IKK inhibitor BMS-345541 on activation of NF-κB and NF-κB-regulated gene end-products in CRC cells: Serum-starved HCT116 cells in alginate cultures from multicellular pro-inflammatory TME cultures were treated as described in Materials and Methods. Immunoblotting of whole-cell lysates from HCT116 was performed for anti-p65-NF-κB, anti-phospho-p65-NF-κB, anti-MMP-9, anti-CXCR4, anti-Cyclin D1, and anti-cleaved-caspase-3. β-actin served as an internal loading control in all experiments. For densitometric evaluation, results are compared to control and p

    Techniques Used: Activation Assay

    Related Articles

    Immunohistochemistry:

    Article Title: Latent Herpes Simplex Virus 1 Infection Does Not Induce Apoptosis in Human Trigeminal Ganglia
    Article Snippet: .. Immunohistochemistry was performed with antibodies against active caspase-3 (R & D Systems, Wiesbaden, Germany) and GrB (AbD Serotec, Puchheim, Germany), as described previously ( , ). .. Staining was visualized using biotin-conjugated secondary antibody (Dako, Hamburg, Germany), horseradish peroxidase (HRP)-conjugated streptavidin (BioLegend, Fell, Germany), and 3-3′-diaminobenzidine (DAB; Dako) under an all-in-one fluorescence microscope (BZ-8100E; Keyence, Neu-Isenburg, Germany).

    Construct:

    Article Title: Induction of ER Stress by an AAV5 BDD FVIII Construct Is Dependent on the Strength of the Hepatic-Specific Promoter
    Article Snippet: .. UPR Selected recognized biomarkers of UPR were determined in the liver in animals administered the highest tested dose of each construct (6 × 1013 vg/kg): GRP78 and spliced XBP1s mRNA by RT-ddPCR, CHOP (loading control TATA-box binding protein), by western blot, and active caspase-3 by ELISA. .. Further groups of animals (n = 2 in each) received tunicamycin, thapsigargin, or staurosporine, all known inducers of ER stress, as “active” controls; tunicamycin and thapsigargin for XBP1s and GRP78 expression; and staurosporine for active caspase-3 expression.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas, et al. Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas
    Article Snippet: .. Active caspase‐3 was quantified by human active caspase‐3 ELISA (R & D Systems), and results were expressed as fold‐increase or fold‐decrease in relation to the control. .. HIF1‐α expression was measured by human HIF1‐α ELISA (Active Motif), and results were expressed as fold‐decrease in relation to the control.

    Article Title: Induction of ER Stress by an AAV5 BDD FVIII Construct Is Dependent on the Strength of the Hepatic-Specific Promoter
    Article Snippet: .. UPR Selected recognized biomarkers of UPR were determined in the liver in animals administered the highest tested dose of each construct (6 × 1013 vg/kg): GRP78 and spliced XBP1s mRNA by RT-ddPCR, CHOP (loading control TATA-box binding protein), by western blot, and active caspase-3 by ELISA. .. Further groups of animals (n = 2 in each) received tunicamycin, thapsigargin, or staurosporine, all known inducers of ER stress, as “active” controls; tunicamycin and thapsigargin for XBP1s and GRP78 expression; and staurosporine for active caspase-3 expression.

    Incubation:

    Article Title: Quiescence and ?H2AX in neuroblastoma are regulated by ouabain/Na,K-ATPase
    Article Snippet: .. Sections from xenograft tumours were incubated with primary antibodies detecting Ki-67 (NeoMarkers), active caspase-3 (R & D Systems, Abingdon, UK) or γH2AX (Ser 139, Cell Signaling). .. Secondary immunostaining was performed using a Superpicture Polymer detection kit (Invitrogen) with antibodies conjugated with horseradish peroxidise (HRP).

    Article Title: Cholera Toxin Induces a Transient Depletion of CD8+ Intraepithelial Lymphocytes in the Rat Small Intestine as Detected by Microarray and Immunohistochemistry
    Article Snippet: .. Sections from control animals and animals exposed to CT for 2 or 6 h were incubated with a polyclonal antibody against active caspase-3 (R & D Systems Europe, Ltd,. ..

    other:

    Article Title: Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1?-induced NF-?B-mediated inflammation and apoptosis
    Article Snippet: Antibodies raised against anti-active caspase-3, MMP-9 and MMP-3 were purchased from R & D Systems (Abingdon, UK).

    Western Blot:

    Article Title: Induction of ER Stress by an AAV5 BDD FVIII Construct Is Dependent on the Strength of the Hepatic-Specific Promoter
    Article Snippet: .. UPR Selected recognized biomarkers of UPR were determined in the liver in animals administered the highest tested dose of each construct (6 × 1013 vg/kg): GRP78 and spliced XBP1s mRNA by RT-ddPCR, CHOP (loading control TATA-box binding protein), by western blot, and active caspase-3 by ELISA. .. Further groups of animals (n = 2 in each) received tunicamycin, thapsigargin, or staurosporine, all known inducers of ER stress, as “active” controls; tunicamycin and thapsigargin for XBP1s and GRP78 expression; and staurosporine for active caspase-3 expression.

    Binding Assay:

    Article Title: Induction of ER Stress by an AAV5 BDD FVIII Construct Is Dependent on the Strength of the Hepatic-Specific Promoter
    Article Snippet: .. UPR Selected recognized biomarkers of UPR were determined in the liver in animals administered the highest tested dose of each construct (6 × 1013 vg/kg): GRP78 and spliced XBP1s mRNA by RT-ddPCR, CHOP (loading control TATA-box binding protein), by western blot, and active caspase-3 by ELISA. .. Further groups of animals (n = 2 in each) received tunicamycin, thapsigargin, or staurosporine, all known inducers of ER stress, as “active” controls; tunicamycin and thapsigargin for XBP1s and GRP78 expression; and staurosporine for active caspase-3 expression.

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  • 99
    R&D Systems anti active caspase 3
    Effects of resveratrol and curcumin on IL-1β-induced NF-κB-dependent gene products in primary chondrocytes. (a) The effect of resveratrol/curcumin on IL-1β-induced NF-κB-dependent anti-apoptotic gene products in primary chondrocytes was studied. To determine whether resveratrol and curcumin treatment actively stimulates the production of anti-apoptotic gene products, primary chondrocyte cultures were either stimulated for 0, 12, 24, and 48 hours with 10 ng/ml IL-1β or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12, 24, and 48 hours stimulation with 10 ng/ml IL-1β. Equal amounts (500 ng protein per lane) of total proteins were separated by 10% SDS-PAGE and analysed by immunoblotting with anti-Bcl-2, anti-Bcl-xL and anti-TNF-α receptor-associated factor 1 (anti-TRAF1) antibodies. A time-dependent downregulation of the expression of Bcl-2, Bcl-xL and TRAF1 by IL-1β was observed. In contrast, pre-treatment with resveratrol and curcumin resulted in a time-dependent increase of these anti-apoptotic proteins. Synthesis of the housekeeping protein β-actin remained unaffected. (b) The effect of resveratrol/curcumin on IL-1β-induced NF-κB-dependent pro-apoptotic protein <t>caspase-3</t> was also studied in primary chondrocytes. Whole cell lysates of primary chondrocyte cultures were either stimulated for 0, 12, 24, and 48 hours with 10 ng/ml IL-1β or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12, 24, and 48 hours of stimulation with 10 ng/ml IL-1β-, and evaluated with western blot analysis to examine the effect on the pro-apoptotic protein caspase-3. Equal amounts (500 ng protein per lane) of total proteins were separated by 12% SDS-PAGE and analysed by immunoblotting with an antibody against active caspase-3. Stimulation of the cultures with IL-1β resulted in a time-dependent activation of caspase-3. In contrast, combinational treatment of resveratrol and curcumin inhibited caspase-3 activation in a time-dependent manner. Synthesis of the housekeeping protein β-actin was not affected.
    Anti Active Caspase 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti active caspase 3/product/R&D Systems
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    anti active caspase 3 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    94
    R&D Systems caspase 3
    Soluble Fas ligand (sFasL) does not activate cell death in airway epithelia. En face confocal images are of epithelia treated with staurosporine or sFasL immunostained for ZO-1 (red) and activated <t>caspase-3</t> (green) at different time points of treatment.
    Caspase 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/R&D Systems
    Average 94 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    caspase 3 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of resveratrol and curcumin on IL-1β-induced NF-κB-dependent gene products in primary chondrocytes. (a) The effect of resveratrol/curcumin on IL-1β-induced NF-κB-dependent anti-apoptotic gene products in primary chondrocytes was studied. To determine whether resveratrol and curcumin treatment actively stimulates the production of anti-apoptotic gene products, primary chondrocyte cultures were either stimulated for 0, 12, 24, and 48 hours with 10 ng/ml IL-1β or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12, 24, and 48 hours stimulation with 10 ng/ml IL-1β. Equal amounts (500 ng protein per lane) of total proteins were separated by 10% SDS-PAGE and analysed by immunoblotting with anti-Bcl-2, anti-Bcl-xL and anti-TNF-α receptor-associated factor 1 (anti-TRAF1) antibodies. A time-dependent downregulation of the expression of Bcl-2, Bcl-xL and TRAF1 by IL-1β was observed. In contrast, pre-treatment with resveratrol and curcumin resulted in a time-dependent increase of these anti-apoptotic proteins. Synthesis of the housekeeping protein β-actin remained unaffected. (b) The effect of resveratrol/curcumin on IL-1β-induced NF-κB-dependent pro-apoptotic protein caspase-3 was also studied in primary chondrocytes. Whole cell lysates of primary chondrocyte cultures were either stimulated for 0, 12, 24, and 48 hours with 10 ng/ml IL-1β or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12, 24, and 48 hours of stimulation with 10 ng/ml IL-1β-, and evaluated with western blot analysis to examine the effect on the pro-apoptotic protein caspase-3. Equal amounts (500 ng protein per lane) of total proteins were separated by 12% SDS-PAGE and analysed by immunoblotting with an antibody against active caspase-3. Stimulation of the cultures with IL-1β resulted in a time-dependent activation of caspase-3. In contrast, combinational treatment of resveratrol and curcumin inhibited caspase-3 activation in a time-dependent manner. Synthesis of the housekeeping protein β-actin was not affected.

    Journal: Arthritis Research & Therapy

    Article Title: Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1?-induced NF-?B-mediated inflammation and apoptosis

    doi: 10.1186/ar2850

    Figure Lengend Snippet: Effects of resveratrol and curcumin on IL-1β-induced NF-κB-dependent gene products in primary chondrocytes. (a) The effect of resveratrol/curcumin on IL-1β-induced NF-κB-dependent anti-apoptotic gene products in primary chondrocytes was studied. To determine whether resveratrol and curcumin treatment actively stimulates the production of anti-apoptotic gene products, primary chondrocyte cultures were either stimulated for 0, 12, 24, and 48 hours with 10 ng/ml IL-1β or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12, 24, and 48 hours stimulation with 10 ng/ml IL-1β. Equal amounts (500 ng protein per lane) of total proteins were separated by 10% SDS-PAGE and analysed by immunoblotting with anti-Bcl-2, anti-Bcl-xL and anti-TNF-α receptor-associated factor 1 (anti-TRAF1) antibodies. A time-dependent downregulation of the expression of Bcl-2, Bcl-xL and TRAF1 by IL-1β was observed. In contrast, pre-treatment with resveratrol and curcumin resulted in a time-dependent increase of these anti-apoptotic proteins. Synthesis of the housekeeping protein β-actin remained unaffected. (b) The effect of resveratrol/curcumin on IL-1β-induced NF-κB-dependent pro-apoptotic protein caspase-3 was also studied in primary chondrocytes. Whole cell lysates of primary chondrocyte cultures were either stimulated for 0, 12, 24, and 48 hours with 10 ng/ml IL-1β or pre-treated with resveratrol and curcumin (50/50 μM) followed by 0, 12, 24, and 48 hours of stimulation with 10 ng/ml IL-1β-, and evaluated with western blot analysis to examine the effect on the pro-apoptotic protein caspase-3. Equal amounts (500 ng protein per lane) of total proteins were separated by 12% SDS-PAGE and analysed by immunoblotting with an antibody against active caspase-3. Stimulation of the cultures with IL-1β resulted in a time-dependent activation of caspase-3. In contrast, combinational treatment of resveratrol and curcumin inhibited caspase-3 activation in a time-dependent manner. Synthesis of the housekeeping protein β-actin was not affected.

    Article Snippet: Antibodies raised against anti-active caspase-3, MMP-9 and MMP-3 were purchased from R & D Systems (Abingdon, UK).

    Techniques: SDS Page, Expressing, Western Blot, Activation Assay

    Immunodetection of active caspase-3 in the jejunum. All panels are light photomicrographs of sections immunostained with a polyclonal antibody against active caspase-3. The tissues were taken from rats given vehicle only (A) and from rats challenged with CT for 2 h (B) or 6 h (C). Magnification, ×10.

    Journal: Infection and Immunity

    Article Title: Cholera Toxin Induces a Transient Depletion of CD8+ Intraepithelial Lymphocytes in the Rat Small Intestine as Detected by Microarray and Immunohistochemistry

    doi: 10.1128/IAI.73.9.5595-5602.2005

    Figure Lengend Snippet: Immunodetection of active caspase-3 in the jejunum. All panels are light photomicrographs of sections immunostained with a polyclonal antibody against active caspase-3. The tissues were taken from rats given vehicle only (A) and from rats challenged with CT for 2 h (B) or 6 h (C). Magnification, ×10.

    Article Snippet: Sections from control animals and animals exposed to CT for 2 or 6 h were incubated with a polyclonal antibody against active caspase-3 (R & D Systems Europe, Ltd,.

    Techniques: Immunodetection

    Box plot A shows the difference in the expression of caspase-3 in LAT + and LAT − single neurons. Box plot B shows the difference in the expression of caspase-3 in LAT + and LAT − whole ganglia. Boxes denote interquartile ranges, lines denote

    Journal: Journal of Virology

    Article Title: Latent Herpes Simplex Virus 1 Infection Does Not Induce Apoptosis in Human Trigeminal Ganglia

    doi: 10.1128/JVI.03481-14

    Figure Lengend Snippet: Box plot A shows the difference in the expression of caspase-3 in LAT + and LAT − single neurons. Box plot B shows the difference in the expression of caspase-3 in LAT + and LAT − whole ganglia. Boxes denote interquartile ranges, lines denote

    Article Snippet: Immunohistochemistry was performed with antibodies against active caspase-3 (R & D Systems, Wiesbaden, Germany) and GrB (AbD Serotec, Puchheim, Germany), as described previously ( , ).

    Techniques: Expressing

    Soluble Fas ligand (sFasL) does not activate cell death in airway epithelia. En face confocal images are of epithelia treated with staurosporine or sFasL immunostained for ZO-1 (red) and activated caspase-3 (green) at different time points of treatment.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: MMP9 modulates tight junction integrity and cell viability in human airway epithelia

    doi: 10.1152/ajplung.90578.2008

    Figure Lengend Snippet: Soluble Fas ligand (sFasL) does not activate cell death in airway epithelia. En face confocal images are of epithelia treated with staurosporine or sFasL immunostained for ZO-1 (red) and activated caspase-3 (green) at different time points of treatment.

    Article Snippet: For immunostaining of activated caspase-3 (1:100 of rabbit anti-human antibody; R & D Systems), epithelia were fixed with 1% paraformaldehyde in methanol for 35 min at −20°C, washed with PBS, and permeabilized with 0.2% Triton X-100 (Pierce).

    Techniques:

    Thrombin treatment does not stimulate epithelial cell death. En face confocal images are of control, staurosporine and thrombin-treated epithelia immunostained for ZO-1 (red) and activated caspase-3 (green) at different time points posttreatment. Control

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: MMP9 modulates tight junction integrity and cell viability in human airway epithelia

    doi: 10.1152/ajplung.90578.2008

    Figure Lengend Snippet: Thrombin treatment does not stimulate epithelial cell death. En face confocal images are of control, staurosporine and thrombin-treated epithelia immunostained for ZO-1 (red) and activated caspase-3 (green) at different time points posttreatment. Control

    Article Snippet: For immunostaining of activated caspase-3 (1:100 of rabbit anti-human antibody; R & D Systems), epithelia were fixed with 1% paraformaldehyde in methanol for 35 min at −20°C, washed with PBS, and permeabilized with 0.2% Triton X-100 (Pierce).

    Techniques:

    MMP9 treatment leads to cell death and apical extrusion. Vertical confocal images immunostained for zonula occludens-1 (ZO-1; red), activated caspase-3 (green), and counterstained with TO-PRO-3 (blue) were taken at different time points following treatment

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: MMP9 modulates tight junction integrity and cell viability in human airway epithelia

    doi: 10.1152/ajplung.90578.2008

    Figure Lengend Snippet: MMP9 treatment leads to cell death and apical extrusion. Vertical confocal images immunostained for zonula occludens-1 (ZO-1; red), activated caspase-3 (green), and counterstained with TO-PRO-3 (blue) were taken at different time points following treatment

    Article Snippet: For immunostaining of activated caspase-3 (1:100 of rabbit anti-human antibody; R & D Systems), epithelia were fixed with 1% paraformaldehyde in methanol for 35 min at −20°C, washed with PBS, and permeabilized with 0.2% Triton X-100 (Pierce).

    Techniques:

    MMP9 and EGTA similarly stimulate airway epithelial cell death. A : confocal 3-dimensional reconstruction of an epithelium treated with MMP9 immunostained for ZO-1 (red) and activated caspase-3 (green) and counterstained with TO-PRO-3 (blue). Activated

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: MMP9 modulates tight junction integrity and cell viability in human airway epithelia

    doi: 10.1152/ajplung.90578.2008

    Figure Lengend Snippet: MMP9 and EGTA similarly stimulate airway epithelial cell death. A : confocal 3-dimensional reconstruction of an epithelium treated with MMP9 immunostained for ZO-1 (red) and activated caspase-3 (green) and counterstained with TO-PRO-3 (blue). Activated

    Article Snippet: For immunostaining of activated caspase-3 (1:100 of rabbit anti-human antibody; R & D Systems), epithelia were fixed with 1% paraformaldehyde in methanol for 35 min at −20°C, washed with PBS, and permeabilized with 0.2% Triton X-100 (Pierce).

    Techniques: