Journal: Neural Regeneration Research

Article Title: Human-induced pluripotent stem cell–derived neural stem cell exosomes improve blood–brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

doi: 10.4103/NRR.NRR-D-23-01889

Figure Lengend Snippet: hiPSC–NSC–Exos suppress leukocyte infiltration on day 3 post-ICH. (A) Flow cytometry analysis of neutrophils (CD45 high CD11b + Ly6G + ), macrophages (CD45 high CD11b + F4/80 + ), CD4 + T cells (CD45 high CD3 + CD4 + ), CD8 + T cells (CD45 high CD3 + CD8 + ), NK cells (CD45 high CD3 – NK1.1 + ), and B cells (CD45 high CD3 – CD19 + ) that had infiltrated the brain tissue. (B) Quantification of leukocyte subsets that had infiltrated the brain tissue ( n = 7). (C) Microglial cell counts ( n = 7). (D) Flow cytometry analysis of neutrophils (CD11b + Ly6G + ), macrophages (CD11b + F4/80 + ), CD4 + T cells (CD3 + CD4 + ), CD8 + T cells (CD3 + CD8 + ), NK cells (CD3 – NK1.1 + ), and B cells (CD3 – CD19 + ) that had infiltrated the spleen. (E) Quantification of immune cell subsets that had infiltrated the mouse spleen ( n = 7). Data are presented as mean ± SEM. ** P < 0.01, *** P < 0.001 (Student’s t -test). All statistical values are presented in . Exo: Exosomes; hiPSC: human induced pluripotent stem cell; ICH: intracerebral hemorrhage; NK: natural killer; ns: not significant; NSC: neural stem cell; PBS: phosphate-buffered saline.

Article Snippet: The CD45 antibody was purchased from Invitrogen (Carlsbad, CA, USA), and all other antibodies were purchased from BioLegend (San Diego, CA, USA).

Techniques: Flow Cytometry, Saline

Journal: Neural Regeneration Research

Article Title: Human-induced pluripotent stem cell–derived neural stem cell exosomes improve blood–brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

doi: 10.4103/NRR.NRR-D-23-01889

Figure Lengend Snippet: Summary of statistical effect sizes in Figure 4

Article Snippet: The CD45 antibody was purchased from Invitrogen (Carlsbad, CA, USA), and all other antibodies were purchased from BioLegend (San Diego, CA, USA).

Techniques: Control