anti caspase 1  (Novus Biologicals)

Journal: bioRxiv

Article Title: HIV-1 Amplifies IL-8 Response Of Human Stellate Cells To Gram-Positive Microbial Products Via H4K5 Histone Acetylation

doi: 10.1101/2025.05.12.653460

Figure Lengend Snippet: A) Total RNA was extracted from HIV-uninfected (n=7) and HIV-1-infected (n=7) liver tissues. mRNA expression of IL-8, α-SMA, collagen I, and TGF-β was quantified by RT-PCR. B) Protein expression of α-SMA and collagen I was examined by western blot; GAPDH served as a loading control. Band intensity was quantified using ImageJ (n>3). C) IL-8 expression was evaluated by immunohistochemistry in HIV-1 infected and HIV-1 non-infected tissues (n=32). The results were quantified by image J D) Serum IL-8 and LTA concentration were measured in duplicate by ELISA and compared across groups stratified by HIV-1 viremia and fibrosis status (n HIV- viremia- = 9, n HIV- viremia-Fib+ = 9, n HIV- viremia+Fib+ = 10).

Article Snippet: Membranes were washed 3 times with blotting buffer, incubated at 4 0 C overnight with primary antibody (1:1000) containing 5% lowfat powdered milk, and incubated with secondary antibody (1:1000) at room temperature for 60 min. Target proteins were developed with TMB 1-component membrane solution (SeraCare Life Sciences Inc., Milford, MA). α-SMA (NOVUS, Cat No. NBP 1-45433 and NB100-56565), Collagen I (NOVUS, Cat No: NBP 2-12446), MMP9 was purchased from Navius Bio.

Techniques: Infection, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Immunohistochemistry, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: HIV-1 Amplifies IL-8 Response Of Human Stellate Cells To Gram-Positive Microbial Products Via H4K5 Histone Acetylation

doi: 10.1101/2025.05.12.653460

Figure Lengend Snippet: A) Lx2 cells were treated with increasing concentrations of LTA for 24 hours. mRNA expression of pro-fibrotic and inflammatory genes was quantified by SYBR Green RT-PCR. B) Lx2 cells were repeatedly treated with IL-8 (200Lpg/ml) or PBS for 3times. Protein expression of α-SMA and collagen I was analyzed by western blot and normalized to GAPDH. Band intensities were quantified using ImageJ (n=3).

Article Snippet: Membranes were washed 3 times with blotting buffer, incubated at 4 0 C overnight with primary antibody (1:1000) containing 5% lowfat powdered milk, and incubated with secondary antibody (1:1000) at room temperature for 60 min. Target proteins were developed with TMB 1-component membrane solution (SeraCare Life Sciences Inc., Milford, MA). α-SMA (NOVUS, Cat No. NBP 1-45433 and NB100-56565), Collagen I (NOVUS, Cat No: NBP 2-12446), MMP9 was purchased from Navius Bio.

Techniques: Expressing, SYBR Green Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot