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5 × 8 Gy SBRT decreases pancreatic tumor hypoxia. ( a ) Intravital fluorescence microscopy of 5xHRE/GFP-expressing BxPC3 tumor cells show a significant decrease in the GFP mean fluorescence intensity (MFI) 1 day post-SBRT. ( b ) Representative images and ( c ) quantification of <t>CA9</t> staining (brown) in ex vivo BxPC3 tumor tissue sections show a significant decrease in tumor cell hypoxia at both 1 and 14 days post-SBRT, compared to non-irradiated control mice. Tissue sections are counterstained with hematoxylin (blue). Scale bar = 200 μm. n = 7 mice per group. *p < 0.05.
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5 × 8 Gy SBRT decreases pancreatic tumor hypoxia. ( a ) Intravital fluorescence microscopy of 5xHRE/GFP-expressing BxPC3 tumor cells show a significant decrease in the GFP mean fluorescence intensity (MFI) 1 day post-SBRT. ( b ) Representative images and ( c ) quantification of CA9 staining (brown) in ex vivo BxPC3 tumor tissue sections show a significant decrease in tumor cell hypoxia at both 1 and 14 days post-SBRT, compared to non-irradiated control mice. Tissue sections are counterstained with hematoxylin (blue). Scale bar = 200 μm. n = 7 mice per group. *p < 0.05.

Journal: Scientific Reports

Article Title: Investigating the effects of stereotactic body radiation therapy on pancreatic tumor hypoxia and microvasculature in an orthotopic mouse model using intravital fluorescence microscopy

doi: 10.1038/s41598-024-82757-1

Figure Lengend Snippet: 5 × 8 Gy SBRT decreases pancreatic tumor hypoxia. ( a ) Intravital fluorescence microscopy of 5xHRE/GFP-expressing BxPC3 tumor cells show a significant decrease in the GFP mean fluorescence intensity (MFI) 1 day post-SBRT. ( b ) Representative images and ( c ) quantification of CA9 staining (brown) in ex vivo BxPC3 tumor tissue sections show a significant decrease in tumor cell hypoxia at both 1 and 14 days post-SBRT, compared to non-irradiated control mice. Tissue sections are counterstained with hematoxylin (blue). Scale bar = 200 μm. n = 7 mice per group. *p < 0.05.

Article Snippet: , Anti-CA9 , Novus Biologicals, NB100-479 , 1:50 , Anti-Rabbit IgG , Thermo Fisher Scientific, A-21428 , Anti-CA9.

Techniques: Fluorescence, Microscopy, Expressing, Staining, Ex Vivo, Irradiation, Control

Antibodies and dilutions used in the study.

Journal: Scientific Reports

Article Title: Investigating the effects of stereotactic body radiation therapy on pancreatic tumor hypoxia and microvasculature in an orthotopic mouse model using intravital fluorescence microscopy

doi: 10.1038/s41598-024-82757-1

Figure Lengend Snippet: Antibodies and dilutions used in the study.

Article Snippet: , Anti-CA9 , Novus Biologicals, NB100-479 , 1:50 , Anti-Rabbit IgG , Thermo Fisher Scientific, A-21428 , Anti-CA9.

Techniques: