adra1b myc  (Promega)

 
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    Structured Review

    Promega adra1b myc
    (A) Co-immunoprecipitation (Co-IP) of <t>ADRA1B-Myc</t> and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. (B) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. (C) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP (F1,32 = 7.899, P < 0.001, n = 5). (D) Interaction between ADRA1B-Myc and GPR143-HA in the presence or absence of L-DOPA. Note that the interaction was enhanced in the presence of L-DOPA for 1 minute. The arrowhead indicates predicted ADRA1B. (E) TIRF microscopic images of GPR143-EGFP (left), ADRA1B-mCherry (center), and merged images (right) as indicated by white arrowheads (n = 3, independent experiments). Scale bar: 3.5 μm. (F) FRET efficiency between Venus and CFP at the plasma membrane. *P < 0.05, **P < 0.01, 1-way ANOVA with Bonferroni’s multiple comparisons test (n = 36–59). n.s., not significant. (G) Displacement binding curve of [H3]-prazosin (0.4 nM) by phenylephrine in HEK293 cells expressing ADRA1B-Myc and GPR143-EGFP or ADRA1B-Myc and free-EGFP (F1,40 = 19.31, ***P < 0.001, n = 3). Two-way ANOVA with Bonferroni’s multiple comparisons test.
    Adra1b Myc, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adra1b myc/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adra1b myc - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor"

    Article Title: L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor

    Journal: JCI Insight

    doi: 10.1172/jci.insight.90903

    (A) Co-immunoprecipitation (Co-IP) of ADRA1B-Myc and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. (B) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. (C) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP (F1,32 = 7.899, P < 0.001, n = 5). (D) Interaction between ADRA1B-Myc and GPR143-HA in the presence or absence of L-DOPA. Note that the interaction was enhanced in the presence of L-DOPA for 1 minute. The arrowhead indicates predicted ADRA1B. (E) TIRF microscopic images of GPR143-EGFP (left), ADRA1B-mCherry (center), and merged images (right) as indicated by white arrowheads (n = 3, independent experiments). Scale bar: 3.5 μm. (F) FRET efficiency between Venus and CFP at the plasma membrane. *P < 0.05, **P < 0.01, 1-way ANOVA with Bonferroni’s multiple comparisons test (n = 36–59). n.s., not significant. (G) Displacement binding curve of [H3]-prazosin (0.4 nM) by phenylephrine in HEK293 cells expressing ADRA1B-Myc and GPR143-EGFP or ADRA1B-Myc and free-EGFP (F1,40 = 19.31, ***P < 0.001, n = 3). Two-way ANOVA with Bonferroni’s multiple comparisons test.
    Figure Legend Snippet: (A) Co-immunoprecipitation (Co-IP) of ADRA1B-Myc and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. (B) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. (C) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP (F1,32 = 7.899, P < 0.001, n = 5). (D) Interaction between ADRA1B-Myc and GPR143-HA in the presence or absence of L-DOPA. Note that the interaction was enhanced in the presence of L-DOPA for 1 minute. The arrowhead indicates predicted ADRA1B. (E) TIRF microscopic images of GPR143-EGFP (left), ADRA1B-mCherry (center), and merged images (right) as indicated by white arrowheads (n = 3, independent experiments). Scale bar: 3.5 μm. (F) FRET efficiency between Venus and CFP at the plasma membrane. *P < 0.05, **P < 0.01, 1-way ANOVA with Bonferroni’s multiple comparisons test (n = 36–59). n.s., not significant. (G) Displacement binding curve of [H3]-prazosin (0.4 nM) by phenylephrine in HEK293 cells expressing ADRA1B-Myc and GPR143-EGFP or ADRA1B-Myc and free-EGFP (F1,40 = 19.31, ***P < 0.001, n = 3). Two-way ANOVA with Bonferroni’s multiple comparisons test.

    Techniques Used: Immunoprecipitation, Co-Immunoprecipitation Assay, Binding Assay, Expressing

    Expression of GPR143 (A) and ADRA1B (C) in the liver (arrowheads). No GPR143 signals were observed in the liver of Gpr143–/y mice (B). No ADRA1B signals were observed in sections incubated with the antigenic peptide–preadsorbed antibody (D). Scale bar: 100 μm. Endogenous interaction between GPR143 and ADRA1B. (E) The GPR143-ADRA1B interacting signals (green) were detected by in situ proximity ligation assay technology in WT liver (arrowheads). (F) No interacting signals were observed in Gpr143–/y liver (n = 2, independent experiments). The nucleus was counterstained by DAPI (blue). Scale bars: 5 μm.
    Figure Legend Snippet: Expression of GPR143 (A) and ADRA1B (C) in the liver (arrowheads). No GPR143 signals were observed in the liver of Gpr143–/y mice (B). No ADRA1B signals were observed in sections incubated with the antigenic peptide–preadsorbed antibody (D). Scale bar: 100 μm. Endogenous interaction between GPR143 and ADRA1B. (E) The GPR143-ADRA1B interacting signals (green) were detected by in situ proximity ligation assay technology in WT liver (arrowheads). (F) No interacting signals were observed in Gpr143–/y liver (n = 2, independent experiments). The nucleus was counterstained by DAPI (blue). Scale bars: 5 μm.

    Techniques Used: Expressing, Incubation, In Situ, Proximity Ligation Assay

    adra1b myc  (Promega)

     
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    Structured Review

    Promega adra1b myc
    (A) Co-immunoprecipitation (Co-IP) of <t>ADRA1B-Myc</t> and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. (B) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. (C) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP (F1,32 = 7.899, P < 0.001, n = 5). (D) Interaction between ADRA1B-Myc and GPR143-HA in the presence or absence of L-DOPA. Note that the interaction was enhanced in the presence of L-DOPA for 1 minute. The arrowhead indicates predicted ADRA1B. (E) TIRF microscopic images of GPR143-EGFP (left), ADRA1B-mCherry (center), and merged images (right) as indicated by white arrowheads (n = 3, independent experiments). Scale bar: 3.5 μm. (F) FRET efficiency between Venus and CFP at the plasma membrane. *P < 0.05, **P < 0.01, 1-way ANOVA with Bonferroni’s multiple comparisons test (n = 36–59). n.s., not significant. (G) Displacement binding curve of [H3]-prazosin (0.4 nM) by phenylephrine in HEK293 cells expressing ADRA1B-Myc and GPR143-EGFP or ADRA1B-Myc and free-EGFP (F1,40 = 19.31, ***P < 0.001, n = 3). Two-way ANOVA with Bonferroni’s multiple comparisons test.
    Adra1b Myc, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adra1b myc/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adra1b myc - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor"

    Article Title: L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor

    Journal: JCI Insight

    doi: 10.1172/jci.insight.90903

    (A) Co-immunoprecipitation (Co-IP) of ADRA1B-Myc and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. (B) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. (C) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP (F1,32 = 7.899, P < 0.001, n = 5). (D) Interaction between ADRA1B-Myc and GPR143-HA in the presence or absence of L-DOPA. Note that the interaction was enhanced in the presence of L-DOPA for 1 minute. The arrowhead indicates predicted ADRA1B. (E) TIRF microscopic images of GPR143-EGFP (left), ADRA1B-mCherry (center), and merged images (right) as indicated by white arrowheads (n = 3, independent experiments). Scale bar: 3.5 μm. (F) FRET efficiency between Venus and CFP at the plasma membrane. *P < 0.05, **P < 0.01, 1-way ANOVA with Bonferroni’s multiple comparisons test (n = 36–59). n.s., not significant. (G) Displacement binding curve of [H3]-prazosin (0.4 nM) by phenylephrine in HEK293 cells expressing ADRA1B-Myc and GPR143-EGFP or ADRA1B-Myc and free-EGFP (F1,40 = 19.31, ***P < 0.001, n = 3). Two-way ANOVA with Bonferroni’s multiple comparisons test.
    Figure Legend Snippet: (A) Co-immunoprecipitation (Co-IP) of ADRA1B-Myc and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. (B) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. (C) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP (F1,32 = 7.899, P < 0.001, n = 5). (D) Interaction between ADRA1B-Myc and GPR143-HA in the presence or absence of L-DOPA. Note that the interaction was enhanced in the presence of L-DOPA for 1 minute. The arrowhead indicates predicted ADRA1B. (E) TIRF microscopic images of GPR143-EGFP (left), ADRA1B-mCherry (center), and merged images (right) as indicated by white arrowheads (n = 3, independent experiments). Scale bar: 3.5 μm. (F) FRET efficiency between Venus and CFP at the plasma membrane. *P < 0.05, **P < 0.01, 1-way ANOVA with Bonferroni’s multiple comparisons test (n = 36–59). n.s., not significant. (G) Displacement binding curve of [H3]-prazosin (0.4 nM) by phenylephrine in HEK293 cells expressing ADRA1B-Myc and GPR143-EGFP or ADRA1B-Myc and free-EGFP (F1,40 = 19.31, ***P < 0.001, n = 3). Two-way ANOVA with Bonferroni’s multiple comparisons test.

    Techniques Used: Immunoprecipitation, Co-Immunoprecipitation Assay, Binding Assay, Expressing

    Expression of GPR143 (A) and ADRA1B (C) in the liver (arrowheads). No GPR143 signals were observed in the liver of Gpr143–/y mice (B). No ADRA1B signals were observed in sections incubated with the antigenic peptide–preadsorbed antibody (D). Scale bar: 100 μm. Endogenous interaction between GPR143 and ADRA1B. (E) The GPR143-ADRA1B interacting signals (green) were detected by in situ proximity ligation assay technology in WT liver (arrowheads). (F) No interacting signals were observed in Gpr143–/y liver (n = 2, independent experiments). The nucleus was counterstained by DAPI (blue). Scale bars: 5 μm.
    Figure Legend Snippet: Expression of GPR143 (A) and ADRA1B (C) in the liver (arrowheads). No GPR143 signals were observed in the liver of Gpr143–/y mice (B). No ADRA1B signals were observed in sections incubated with the antigenic peptide–preadsorbed antibody (D). Scale bar: 100 μm. Endogenous interaction between GPR143 and ADRA1B. (E) The GPR143-ADRA1B interacting signals (green) were detected by in situ proximity ligation assay technology in WT liver (arrowheads). (F) No interacting signals were observed in Gpr143–/y liver (n = 2, independent experiments). The nucleus was counterstained by DAPI (blue). Scale bars: 5 μm.

    Techniques Used: Expressing, Incubation, In Situ, Proximity Ligation Assay

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