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Becton Dickinson anti actin ab 5
Anti Actin Ab 5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti actin ab 5/product/Becton Dickinson
Average 88 stars, based on 3 article reviews
Price from $9.99 to $1999.99
anti actin ab 5 - by Bioz Stars, 2020-09
88/100 stars

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Positive Control:

Article Title: Effects of aldosterone and related steroids on LPS-induced increased expression of inducible NOS in rat aortic smooth muscle cells
Article Snippet: .. Murine-derived (IgG1) primary antibodies were anti-iNOS (Cat. No: 610431) and anti-actin Ab-5 (Cat. No: 612656) from Becton Dickinson (Cowley, UK), as also was iNOS ‘positive control’ mouse macrophage lysate (Cat. No: 611473). .. Secondary antibody was horseradish peroxidase-conjugated rabbit anti-mouse IgG (Cat. No: 31450) from Pierce Biotech. via Thermo Fisher (CramLington, UK).

SDS Page:

Article Title: Human Airway Eosinophils Exhibit Preferential Reduction in STAT Signaling Capacity and Increased CISH Expression
Article Snippet: .. Supernatants were assayed for total protein content using the Pierce Micro BCA protein assay (Rockford, IL), electrophoresed on SDS-PAGE gels and immunoblotted as described in the figure legends using antibodies against the following: phospho-Tyr 695 STAT5 and phospho-Tyr 705 STAT3 (Cell Signaling Technology, Danvers, MA), phospho-Thr 185,-Tyr 187 ERK1/2 (Invitrogen, Carlsbad, CA), CISH (R & D Systems, Minneapolis, MN), STAT5 and STAT3 (Santa Cruz Biotechnology, Santa Cruz, CA) and Actin Ab-5 (BD Biosciences, San Jose, CA). ..

Immunoprecipitation:

Article Title: WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton
Article Snippet: .. Antibodies Anti-myc tag (clone4A6) for immunoprecipitation, anti-v-Src (clone 327) were purchased from Merck Millipore and Thermo Fisher; c-Myc(9E10) HRP for western blotting, VSV-G(P5D4), WDR1(G-13), anti-calnexin, p-Tyr(PY99) HRP, anti-vimentin, anti-GAPDH and secondary antibodies coupled with HRP from Santa Cruz Biotechnology Inc; Anti-WDR1 Atlas Antibodies; Anti-Actin Ab-5 (Clone C4/actin) from BD Biosciences; Anti-EYA3 from Abcam, Proteintech and Atlas Antibodies, anti-calnexin from Abcam; GST-tagged active v-Src kinase protein from SignalChem; Anti-His antibody from GE Healthcare. .. Constructs The cDNA sequences of both human EYA1 (Q99502) and human EYA3 (Q99504) were amplified by PCR from the Human Transcriptome cDNA library from Stratagene.

Article Title: WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton
Article Snippet: .. Anti-myc tag (clone4A6) for immunoprecipitation, anti-v-Src (clone 327) were purchased from Merck Millipore and Thermo Fisher; c-Myc(9E10) HRP for western blotting, VSV-G(P5D4), WDR1(G-13), anti-calnexin, p-Tyr(PY99) HRP, anti-vimentin, anti-GAPDH and secondary antibodies coupled with HRP from Santa Cruz Biotechnology Inc; Anti-WDR1 Atlas Antibodies; Anti-Actin Ab-5 (Clone C4/actin) from BD Biosciences; Anti-EYA3 from Abcam, Proteintech and Atlas Antibodies, anti-calnexin from Abcam; GST-tagged active v-Src kinase protein from SignalChem; Anti-His antibody from GE Healthcare. .. The cDNA sequences of both human EYA1 ( ) and human EYA3 ( ) were amplified by PCR from the Human Transcriptome cDNA library from Stratagene.

Incubation:

Article Title: JunB promotes Th17 cell identity and restrains alternative CD4+ T-cell programs during inflammation
Article Snippet: .. Membranes were blocked with TBST containing 5% nonfat dry milk, and subsequently, incubated with anti-JunB (N-17; sc-46x; 1 : 2000) or anti-Actin Ab-5 (C4/actin; BD Transduction laboratories; 1 : 2000) as a loading control. .. ECL western blotting substrate (Thermo Fisher Scientific) was used in accordance with the manufacurer’s instructions, for detection of horseradishperioxidase labeled secondary antibodies.

BIA-KA:

Article Title: Human Airway Eosinophils Exhibit Preferential Reduction in STAT Signaling Capacity and Increased CISH Expression
Article Snippet: .. Supernatants were assayed for total protein content using the Pierce Micro BCA protein assay (Rockford, IL), electrophoresed on SDS-PAGE gels and immunoblotted as described in the figure legends using antibodies against the following: phospho-Tyr 695 STAT5 and phospho-Tyr 705 STAT3 (Cell Signaling Technology, Danvers, MA), phospho-Thr 185,-Tyr 187 ERK1/2 (Invitrogen, Carlsbad, CA), CISH (R & D Systems, Minneapolis, MN), STAT5 and STAT3 (Santa Cruz Biotechnology, Santa Cruz, CA) and Actin Ab-5 (BD Biosciences, San Jose, CA). ..

Western Blot:

Article Title: Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation
Article Snippet: .. Antibodies, Enzymes, Reagents, and Kits Antibodies used in Western blotting: anti-pY (pY99, sc-7020 HRP), anti-GAPDH (FL-335) HRP (sc-25778 HRP), anti-c-Myc (9E10) HRP (sc-40 HRP), and secondary antibodies goat anti-rabbit IgG-HRP (sc-2004) and rabbit anti-mouse IgG-HRP (sc-358914) from Santa Cruz Biotechnology, Dallas, TX, USA, anti-His antibody (27-4710-01), from GE Healthcare, Chicago, IL, USA, anti-v-Src (clone 327) from Merck Millipore, Burlington, MA, USA, and anti-Actin Ab-5 (Clone C4/actin) from BD Biosciences, Bedford, MA, USA. .. Anti-c-Myc (9E10) (sc-40) from Santa Cruz Biotechnology and anti-myc tag (clone4A6) from Merck Millipore were used for immunoprecipitation (IP).

Article Title: WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton
Article Snippet: .. Antibodies Anti-myc tag (clone4A6) for immunoprecipitation, anti-v-Src (clone 327) were purchased from Merck Millipore and Thermo Fisher; c-Myc(9E10) HRP for western blotting, VSV-G(P5D4), WDR1(G-13), anti-calnexin, p-Tyr(PY99) HRP, anti-vimentin, anti-GAPDH and secondary antibodies coupled with HRP from Santa Cruz Biotechnology Inc; Anti-WDR1 Atlas Antibodies; Anti-Actin Ab-5 (Clone C4/actin) from BD Biosciences; Anti-EYA3 from Abcam, Proteintech and Atlas Antibodies, anti-calnexin from Abcam; GST-tagged active v-Src kinase protein from SignalChem; Anti-His antibody from GE Healthcare. .. Constructs The cDNA sequences of both human EYA1 (Q99502) and human EYA3 (Q99504) were amplified by PCR from the Human Transcriptome cDNA library from Stratagene.

Article Title: WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton
Article Snippet: .. Anti-myc tag (clone4A6) for immunoprecipitation, anti-v-Src (clone 327) were purchased from Merck Millipore and Thermo Fisher; c-Myc(9E10) HRP for western blotting, VSV-G(P5D4), WDR1(G-13), anti-calnexin, p-Tyr(PY99) HRP, anti-vimentin, anti-GAPDH and secondary antibodies coupled with HRP from Santa Cruz Biotechnology Inc; Anti-WDR1 Atlas Antibodies; Anti-Actin Ab-5 (Clone C4/actin) from BD Biosciences; Anti-EYA3 from Abcam, Proteintech and Atlas Antibodies, anti-calnexin from Abcam; GST-tagged active v-Src kinase protein from SignalChem; Anti-His antibody from GE Healthcare. .. The cDNA sequences of both human EYA1 ( ) and human EYA3 ( ) were amplified by PCR from the Human Transcriptome cDNA library from Stratagene.

Article Title: A new mitochondrial pool of cyclin E, regulated by Drp1, is linked to cell-density-dependent cell proliferation
Article Snippet: .. Primary antibodies were against: 44/42 MAPK (ERK1/2) (western blotting, 1:1000; immunofluorescence, 1:100) phosphorylated p44/42 MAPK (western blotting, 1:1000; immunofluorescence, 1:100) CycE1 (HE12) (western blotting, 1:1000; immunofluorescence 1:100) Cyclin A (BF683) (1:300) Cyclin B1 (1:1000) p-CycE1(T62) (western blotting, 1:500; immunofluorescence 1:100) and CDK2 (1:10000) (Cell Signaling); actin AB-5 (1:10000; BD Biociences), Tom20 (western blotting, 1:10000; immunofluorescence, 1:200) and DmCycE (d-300; 1:100) (Santa Cruz Biotechnology), Sox2 (western blotting, 1:1000; immunofluorescence, 1:300; BD Bioscience), HSP-60 (1:200; BD Bioscience), ATP-B (1:100; Abcam) and Geminin (1:100; a gift from Mary Lilly, Section of Gamete Development, NICHD, Bethesda, USA). ..

Chromogenic In Situ Hybridization:

Article Title: Human Airway Eosinophils Exhibit Preferential Reduction in STAT Signaling Capacity and Increased CISH Expression
Article Snippet: .. Supernatants were assayed for total protein content using the Pierce Micro BCA protein assay (Rockford, IL), electrophoresed on SDS-PAGE gels and immunoblotted as described in the figure legends using antibodies against the following: phospho-Tyr 695 STAT5 and phospho-Tyr 705 STAT3 (Cell Signaling Technology, Danvers, MA), phospho-Thr 185,-Tyr 187 ERK1/2 (Invitrogen, Carlsbad, CA), CISH (R & D Systems, Minneapolis, MN), STAT5 and STAT3 (Santa Cruz Biotechnology, Santa Cruz, CA) and Actin Ab-5 (BD Biosciences, San Jose, CA). ..

Immunofluorescence:

Article Title: A new mitochondrial pool of cyclin E, regulated by Drp1, is linked to cell-density-dependent cell proliferation
Article Snippet: .. Primary antibodies were against: 44/42 MAPK (ERK1/2) (western blotting, 1:1000; immunofluorescence, 1:100) phosphorylated p44/42 MAPK (western blotting, 1:1000; immunofluorescence, 1:100) CycE1 (HE12) (western blotting, 1:1000; immunofluorescence 1:100) Cyclin A (BF683) (1:300) Cyclin B1 (1:1000) p-CycE1(T62) (western blotting, 1:500; immunofluorescence 1:100) and CDK2 (1:10000) (Cell Signaling); actin AB-5 (1:10000; BD Biociences), Tom20 (western blotting, 1:10000; immunofluorescence, 1:200) and DmCycE (d-300; 1:100) (Santa Cruz Biotechnology), Sox2 (western blotting, 1:1000; immunofluorescence, 1:300; BD Bioscience), HSP-60 (1:200; BD Bioscience), ATP-B (1:100; Abcam) and Geminin (1:100; a gift from Mary Lilly, Section of Gamete Development, NICHD, Bethesda, USA). ..

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    Becton Dickinson actin ab 5
    Actin Ab 5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/actin ab 5/product/Becton Dickinson
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    actin ab 5 - by Bioz Stars, 2020-09
    85/100 stars
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