antartic phosphatase buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs antartic phosphatase buffer
    Antartic Phosphatase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antartic phosphatase buffer/product/New England Biolabs
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    antartic phosphatase buffer - by Bioz Stars, 2020-02
    78/100 stars

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    Polymerase Chain Reaction:

    Article Title: Is Bocourt's Terrific Skink Really So Terrific? Trophic Myth and Reality
    Article Snippet: .. Excess primers and dNTPs were removed from the PCR product using an enzymatic reaction; Escherichia coli exonuclease I, Antartic phosphatase, and Antartic phosphatase buffer (all New England Biolabs) were added to each sample. .. Sequencing was carried out in both directions using the BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Treerunners, cryptic lizards of the Plica plica group (Squamata, Sauria, Tropiduridae) of northern South America
    Article Snippet: .. Following the PCR, excess primers and dNTPs were removed using enzymatic reaction of Escherichia coli Exonuclease I, Antartic phosphatase and Antartic phosphatase buffer (all New England Biolabs). .. Sequencing was carried out in both directions using the BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems) according to the manufacturer’s instructions.

    Sequencing:

    Article Title: Is Bocourt's Terrific Skink Really So Terrific? Trophic Myth and Reality
    Article Snippet: Excess primers and dNTPs were removed from the PCR product using an enzymatic reaction; Escherichia coli exonuclease I, Antartic phosphatase, and Antartic phosphatase buffer (all New England Biolabs) were added to each sample. .. Sequencing was carried out in both directions using the BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Treerunners, cryptic lizards of the Plica plica group (Squamata, Sauria, Tropiduridae) of northern South America
    Article Snippet: Following the PCR, excess primers and dNTPs were removed using enzymatic reaction of Escherichia coli Exonuclease I, Antartic phosphatase and Antartic phosphatase buffer (all New England Biolabs). .. Sequencing was carried out in both directions using the BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems) according to the manufacturer’s instructions.

    Amplification:

    Article Title: Is Bocourt's Terrific Skink Really So Terrific? Trophic Myth and Reality
    Article Snippet: The following primers were used: 12S rDNA: 12SA 5´-AAACTGGGATTAGATACCCCACTAT-3´ and 12SB 5´-GAGGGTGACGGGCGGTGTGT-3´ [ ] amplified a ~400 base pair fragment while 16S rDNA 5´-GCCTGTTTATCAAAAACAT-3´ and 16SH 5´-CCGGTCTGAACTCAGATCACGT- 3´ [ ] amplified a ~700 base pair fragment. .. Excess primers and dNTPs were removed from the PCR product using an enzymatic reaction; Escherichia coli exonuclease I, Antartic phosphatase, and Antartic phosphatase buffer (all New England Biolabs) were added to each sample.

    Article Title: Treerunners, cryptic lizards of the Plica plica group (Squamata, Sauria, Tropiduridae) of northern South America
    Article Snippet: Following the PCR, excess primers and dNTPs were removed using enzymatic reaction of Escherichia coli Exonuclease I, Antartic phosphatase and Antartic phosphatase buffer (all New England Biolabs). .. The primers for the12S rDNA: 12SA 5´- AAACTGGGATTAGATACCCCACTAT- 3´, 12SB 5´- GAGGGTGACGGGCGGTGTGT-3´ and 16S rDNA: 5´- GCCTGTTTATCAAAAACAT-3´, 16SH 5´- CCGGTCTGAACTCAGATCACGT- 3´ amplified approximately a 400 and 500 respectively base pair fraction (Genbank accessions: 12S rDNA; KU 895880, 16S rDNA; KU 895881).

    Labeling:

    Article Title: Is Bocourt's Terrific Skink Really So Terrific? Trophic Myth and Reality
    Article Snippet: Excess primers and dNTPs were removed from the PCR product using an enzymatic reaction; Escherichia coli exonuclease I, Antartic phosphatase, and Antartic phosphatase buffer (all New England Biolabs) were added to each sample. .. Labeled fragments were resolved on an automated A3130xl genetic analyzer (Applied Biosystems).

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  • 95
    New England Biolabs antarctic phosphatase buffer
    Antarctic Phosphatase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antarctic phosphatase buffer/product/New England Biolabs
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    antarctic phosphatase buffer - by Bioz Stars, 2020-02
    95/100 stars
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