antarctic phosphatase reaction buffer  (New England Biolabs)


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    Name:
    Antarctic Phosphatase Reaction Buffer
    Description:
    Antarctic Phosphatase Reaction Buffer 6 0 ml
    Catalog Number:
    B0289S
    Price:
    24
    Category:
    Buffers
    Size:
    6 0 ml
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    New England Biolabs antarctic phosphatase reaction buffer
    Antarctic Phosphatase Reaction Buffer
    Antarctic Phosphatase Reaction Buffer 6 0 ml
    https://www.bioz.com/result/antarctic phosphatase reaction buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antarctic phosphatase reaction buffer - by Bioz Stars, 2021-07
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Molecular Characterization of the Role of the Schizosaccharomyces pombe nip1+/ctp1+ Gene in DNA Double-Strand Break Repair in Association with the Mre11-Rad50-Nbs1 Complex ▿
    Article Snippet: The beads were washed with HB buffer, and the precipitates were subjected to Western blotting. .. When phosphatase treatment was performed, lambda phosphatase (NEB) was added to a reaction mixture containing precipitated beads in 10 μl phosphatase buffer (NEB) at 30°C for 30 min, followed by Western blotting analysis. .. The ECL Plus system (GE Healthcare Bio-Science) was used for detection of immunocomplexes.

    Plasmid Preparation:

    Article Title: Poliovirus: Generation and Characterization of Mutants
    Article Snippet: .. 10 ng/μL cDNA plasmid template 10 μM For PCR primer 10 μM Rev PCR primer 10 μM For mutagenesis primer 10 μM Rev mutagenesis primer Herculase II Fusion DNA Polymerase 5× Herculase II reaction buffer (provided with Herculase II Fusion DNA Polymerase) dNTP mix (10 mM each dNTP) Nuclease-free water 1% agarose TAE gels and running buffer ( ) 6× DNA Loading Dye DNA Ladder (e.g. GeneRuler 1 kb Plus, Fermentas) Gel and PCR Cleanup Kit (e.g. Nucleospin Gel and PCR Clean-up, Machery-Nagel) ≥10 μg cDNA plasmid vector Restriction enzymes Buffer(s) for restriction enzymes (provided with enzyme(s)) BSA (if needed; provided with enzyme(s)) 5 U/μL Antarctic Phosphatase (NEB) 10× Antarctic Phosphatase Reaction Buffer (provided with 5U/μL Antarctic Phosphatase) Quick Ligation Kit (NEB; includes Quick T4 DNA Ligase and 2× Quick Ligation Reaction Buffer) SURE Electroporation-competent Cells (Agilent) Sterile water LB medium LB-Amp agar plates (50–100 μg/mL) LB-Amp medium (50–100 μg/mL) Mini-prep Kit (e.g. NucleoSpin Plasmid, Machery-Nagel) 1× TE (pH 8.0) Ice Electroporator and 1 mm cuvettes .. 1 Prepare 2 separate PCR reactions, one with each of these primer pairs: For PCR primer & Rev mutagenesis primer, For mutagenesis primer & Rev PCR primer.

    Polymerase Chain Reaction:

    Article Title: Poliovirus: Generation and Characterization of Mutants
    Article Snippet: .. 10 ng/μL cDNA plasmid template 10 μM For PCR primer 10 μM Rev PCR primer 10 μM For mutagenesis primer 10 μM Rev mutagenesis primer Herculase II Fusion DNA Polymerase 5× Herculase II reaction buffer (provided with Herculase II Fusion DNA Polymerase) dNTP mix (10 mM each dNTP) Nuclease-free water 1% agarose TAE gels and running buffer ( ) 6× DNA Loading Dye DNA Ladder (e.g. GeneRuler 1 kb Plus, Fermentas) Gel and PCR Cleanup Kit (e.g. Nucleospin Gel and PCR Clean-up, Machery-Nagel) ≥10 μg cDNA plasmid vector Restriction enzymes Buffer(s) for restriction enzymes (provided with enzyme(s)) BSA (if needed; provided with enzyme(s)) 5 U/μL Antarctic Phosphatase (NEB) 10× Antarctic Phosphatase Reaction Buffer (provided with 5U/μL Antarctic Phosphatase) Quick Ligation Kit (NEB; includes Quick T4 DNA Ligase and 2× Quick Ligation Reaction Buffer) SURE Electroporation-competent Cells (Agilent) Sterile water LB medium LB-Amp agar plates (50–100 μg/mL) LB-Amp medium (50–100 μg/mL) Mini-prep Kit (e.g. NucleoSpin Plasmid, Machery-Nagel) 1× TE (pH 8.0) Ice Electroporator and 1 mm cuvettes .. 1 Prepare 2 separate PCR reactions, one with each of these primer pairs: For PCR primer & Rev mutagenesis primer, For mutagenesis primer & Rev PCR primer.

    Mutagenesis:

    Article Title: Poliovirus: Generation and Characterization of Mutants
    Article Snippet: .. 10 ng/μL cDNA plasmid template 10 μM For PCR primer 10 μM Rev PCR primer 10 μM For mutagenesis primer 10 μM Rev mutagenesis primer Herculase II Fusion DNA Polymerase 5× Herculase II reaction buffer (provided with Herculase II Fusion DNA Polymerase) dNTP mix (10 mM each dNTP) Nuclease-free water 1% agarose TAE gels and running buffer ( ) 6× DNA Loading Dye DNA Ladder (e.g. GeneRuler 1 kb Plus, Fermentas) Gel and PCR Cleanup Kit (e.g. Nucleospin Gel and PCR Clean-up, Machery-Nagel) ≥10 μg cDNA plasmid vector Restriction enzymes Buffer(s) for restriction enzymes (provided with enzyme(s)) BSA (if needed; provided with enzyme(s)) 5 U/μL Antarctic Phosphatase (NEB) 10× Antarctic Phosphatase Reaction Buffer (provided with 5U/μL Antarctic Phosphatase) Quick Ligation Kit (NEB; includes Quick T4 DNA Ligase and 2× Quick Ligation Reaction Buffer) SURE Electroporation-competent Cells (Agilent) Sterile water LB medium LB-Amp agar plates (50–100 μg/mL) LB-Amp medium (50–100 μg/mL) Mini-prep Kit (e.g. NucleoSpin Plasmid, Machery-Nagel) 1× TE (pH 8.0) Ice Electroporator and 1 mm cuvettes .. 1 Prepare 2 separate PCR reactions, one with each of these primer pairs: For PCR primer & Rev mutagenesis primer, For mutagenesis primer & Rev PCR primer.

    Ligation:

    Article Title: Poliovirus: Generation and Characterization of Mutants
    Article Snippet: .. 10 ng/μL cDNA plasmid template 10 μM For PCR primer 10 μM Rev PCR primer 10 μM For mutagenesis primer 10 μM Rev mutagenesis primer Herculase II Fusion DNA Polymerase 5× Herculase II reaction buffer (provided with Herculase II Fusion DNA Polymerase) dNTP mix (10 mM each dNTP) Nuclease-free water 1% agarose TAE gels and running buffer ( ) 6× DNA Loading Dye DNA Ladder (e.g. GeneRuler 1 kb Plus, Fermentas) Gel and PCR Cleanup Kit (e.g. Nucleospin Gel and PCR Clean-up, Machery-Nagel) ≥10 μg cDNA plasmid vector Restriction enzymes Buffer(s) for restriction enzymes (provided with enzyme(s)) BSA (if needed; provided with enzyme(s)) 5 U/μL Antarctic Phosphatase (NEB) 10× Antarctic Phosphatase Reaction Buffer (provided with 5U/μL Antarctic Phosphatase) Quick Ligation Kit (NEB; includes Quick T4 DNA Ligase and 2× Quick Ligation Reaction Buffer) SURE Electroporation-competent Cells (Agilent) Sterile water LB medium LB-Amp agar plates (50–100 μg/mL) LB-Amp medium (50–100 μg/mL) Mini-prep Kit (e.g. NucleoSpin Plasmid, Machery-Nagel) 1× TE (pH 8.0) Ice Electroporator and 1 mm cuvettes .. 1 Prepare 2 separate PCR reactions, one with each of these primer pairs: For PCR primer & Rev mutagenesis primer, For mutagenesis primer & Rev PCR primer.

    Electroporation:

    Article Title: Poliovirus: Generation and Characterization of Mutants
    Article Snippet: .. 10 ng/μL cDNA plasmid template 10 μM For PCR primer 10 μM Rev PCR primer 10 μM For mutagenesis primer 10 μM Rev mutagenesis primer Herculase II Fusion DNA Polymerase 5× Herculase II reaction buffer (provided with Herculase II Fusion DNA Polymerase) dNTP mix (10 mM each dNTP) Nuclease-free water 1% agarose TAE gels and running buffer ( ) 6× DNA Loading Dye DNA Ladder (e.g. GeneRuler 1 kb Plus, Fermentas) Gel and PCR Cleanup Kit (e.g. Nucleospin Gel and PCR Clean-up, Machery-Nagel) ≥10 μg cDNA plasmid vector Restriction enzymes Buffer(s) for restriction enzymes (provided with enzyme(s)) BSA (if needed; provided with enzyme(s)) 5 U/μL Antarctic Phosphatase (NEB) 10× Antarctic Phosphatase Reaction Buffer (provided with 5U/μL Antarctic Phosphatase) Quick Ligation Kit (NEB; includes Quick T4 DNA Ligase and 2× Quick Ligation Reaction Buffer) SURE Electroporation-competent Cells (Agilent) Sterile water LB medium LB-Amp agar plates (50–100 μg/mL) LB-Amp medium (50–100 μg/mL) Mini-prep Kit (e.g. NucleoSpin Plasmid, Machery-Nagel) 1× TE (pH 8.0) Ice Electroporator and 1 mm cuvettes .. 1 Prepare 2 separate PCR reactions, one with each of these primer pairs: For PCR primer & Rev mutagenesis primer, For mutagenesis primer & Rev PCR primer.

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  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 95
    New England Biolabs phosphatase reaction
    Phosphatase Reaction, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase reaction/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatase reaction - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

    94
    New England Biolabs antarctic phosphatase reaction buffer
    Antarctic Phosphatase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antarctic phosphatase reaction buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antarctic phosphatase reaction buffer - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

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