antibodies against nkcc2  (Alomone Labs)

Journal: Pflugers Archiv

Article Title: Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb

doi: 10.1007/s00424-025-03086-4

Figure Lengend Snippet: Imaging pipeline. Cryosections of mouse kidneys were placed on glass slides (top panel) and then processed for RNAscope detection of various mRNAs, combined with immunofluorescent detection of ROMK (lower left panel), for 4i multiplexing immunodetection of several antigens, including ROMK (lower middle panel), or for automated image analysis (lower right panel). For the latter, immunofluorescent stainings for ROMK, NKCC2, and NOS1 were converted to binaries using supervised machine learning (Ilastik) and then analyzed for automated quantification of the cell surface abundance of ROMK

Article Snippet: C , D Close-ups showing TALs stained with our previously published antibodies against NKCC2 and ROMK (Table ); insert in D immunodetection of ROMK with the commercially available antibody from Alomone (Table ); arrowheads – apical localization of ROMK; arrows – perinuclear localization of ROMK Fig. 3 Co-detection of ROMK protein and Kcnj1 mRNA.

Techniques: Imaging, RNAscope, Multiplexing, Immunodetection

Journal: Pflugers Archiv

Article Title: Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb

doi: 10.1007/s00424-025-03086-4

Figure Lengend Snippet: Quantification of ROMK immunostainings. A Percentage of the ROMK-positive apical area in NKCC2-positive TALs in the renal cortex, OMOS, OMIS, and in the macula densa (MD). B Intensity of ROMK immunofluorescence in the cortical TAL and the macula densa (MD) in arbitrary units. Data are presented as means ± standard error of mean; each data point per bar represents a different mouse

Article Snippet: C , D Close-ups showing TALs stained with our previously published antibodies against NKCC2 and ROMK (Table ); insert in D immunodetection of ROMK with the commercially available antibody from Alomone (Table ); arrowheads – apical localization of ROMK; arrows – perinuclear localization of ROMK Fig. 3 Co-detection of ROMK protein and Kcnj1 mRNA.

Techniques: Immunofluorescence

Journal: Pflugers Archiv

Article Title: Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb

doi: 10.1007/s00424-025-03086-4

Figure Lengend Snippet: Immunodetection of ROMK protein at the macula densa. A Immunohistochemical stainings with the commercially available ROMK antibody (Table ); B Immunohistochemical co-staining for NKCC2 (magenta), ROMK (yellow), NOS1 (green) using our previously published ROMK antibody (Table ); arrowheads mark the region of the macula densa (MD); G – glomerulus; scale bar = 50 μm

Article Snippet: C , D Close-ups showing TALs stained with our previously published antibodies against NKCC2 and ROMK (Table ); insert in D immunodetection of ROMK with the commercially available antibody from Alomone (Table ); arrowheads – apical localization of ROMK; arrows – perinuclear localization of ROMK Fig. 3 Co-detection of ROMK protein and Kcnj1 mRNA.

Techniques: Immunodetection, Immunohistochemical staining, Staining

Journal: Pflugers Archiv : European journal of physiology

Article Title: Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice.

doi: 10.1007/s00424-021-02611-5

Figure Lengend Snippet: Fig. 6 Effects of LK intake on expression of NHE3, NHE2, and NKCC2 in mouse kidney. NHE3, NHE2, and NKCC2 protein expres- sion in male and female mice fed with control-K (CK) or low-K (LK) diets for 7 days. A: Representative Western blots for proteins abun- dance. Blots were stained with anti-NHE3 (3H3, 1:1000), NHE2

Article Snippet: Blots were stained with anti-NHE3 (3H3, 1:1000), NHE2 (Alomone, 1:200), and NKCC2 (Chemicon, 1:1000).

Techniques: Expressing, Control, Western Blot, Staining