anti-p75 ngf receptor -fitc antibody  (Alomone Labs)

Journal: Biomedicines

Article Title: A Possible Role for Nerve Growth Factor and Its Receptors in Human Sperm Pathology

doi: 10.3390/biomedicines11123345

Figure Lengend Snippet: Nerve growth factor (NGF) receptors in the ejaculated sperm, epididymis and testis of fertile men. ( A ) The bar graph shows the mean and standard deviation of the fold increase in TrKA (slanted-line bar graph) and p75 NTR (checkerboard bar graph) messenger RNA (mRNA) levels in fertile men ( n = 7). ( B , C ) The plots (blue gate) display the flow cytometric analysis (representative of one fertile man) measuring the size and granularity of sperm ( B ) and their extracellular expression of NGF receptors ( C ). ( D – F ) Fluorescence immunostaining of human sperm from fertile men incubated with the anti-TrKA ( D , E ) and anti-p75 NTR ( F ) antibodies. In ( D , E ), the labelling is evident throughout the entire tail and in the post-acrosomal sheath (( E ), arrow). In ( F ), there is weak staining throughout the entire tail. The nuclei are stained with DAPI, except in ( E ) where the localisation of post-acrosomal sheath is shown. ( G – J ) Immunolocalisation of the anti-TrKA and anti-p75 NTR antibodies in the epididymis ( G and H , respectively) and testis ( I and J , respectively) from a fertile man. Both receptors appear in an apical position of the epididymal epithelium (( G ): TrKA, ( H ): p75 NTR ). There is also clear localisation of both antibodies in the interstitial tissue of the testis (asterisks, ( I ): TrKA, ( J ): p75 NTR ). Bars: 5 µm ( D – F ), 50 µm ( G – J ).

Article Snippet: After washing three times in PBS supplemented with BSA, sperm were incubated at 4 °C for 1 h in PBS/BSA containing FITC-labelled rabbit anti-human p75 NTR extracellular domain (ANT-007-F, Alomone Labs, Jerusalem, Israel) (10 mL/sample) and PE-labelled mouse anti-human TrKA (FAB1751P, R&D Systems, Minneapolis, MN, USA) (10 mL/sample).

Techniques: Standard Deviation, Expressing, Fluorescence, Immunostaining, Incubation, Staining

Journal: Biomedicines

Article Title: A Possible Role for Nerve Growth Factor and Its Receptors in Human Sperm Pathology

doi: 10.3390/biomedicines11123345

Figure Lengend Snippet: Nerve growth factor (NGF) receptors in altered sperm from men with infertility. ( A – D ) Fluorescence immunostaining of human sperm from men with infertility (varicocele (V) or urogenital infections (UGIs)) incubated with the anti-TrKA ( A , B ) and anti-p75 NTR ( C , D ) antibodies. In ( A ) (sperm from the UGI group), the TrkA signal is evident in the midpiece of the sperm (arrow); in ( B ) (sperm from V group), there is staining in the midpiece and in the entire coiled tail (asterisk). In ( C , D ), p75 NTR staining is strong in the midpiece and cytoplasmic residue (arrow, sperm from the V group) and in the entire coiled tail (asterisk) and midpiece (( D ), from the UGI group). Bars: 5 µm. ( E , F ) The bar graphs show the mean and standard deviation of the fold change in the TrKA ( E ) and p75 NTR ( F ) mRNA level for fertile men ( n = 7, F, white bars), men with varicocele ( n = 7, V, grey bars) and men with UGI ( n = 7, black bars). One-way analysis of variance followed by the Tukey test was used for multiple comparisons (α = 0.05 and 95% confidence interval). The significant p value (<0.05) is indicated.

Article Snippet: After washing three times in PBS supplemented with BSA, sperm were incubated at 4 °C for 1 h in PBS/BSA containing FITC-labelled rabbit anti-human p75 NTR extracellular domain (ANT-007-F, Alomone Labs, Jerusalem, Israel) (10 mL/sample) and PE-labelled mouse anti-human TrKA (FAB1751P, R&D Systems, Minneapolis, MN, USA) (10 mL/sample).

Techniques: Fluorescence, Immunostaining, Incubation, Staining, Residue, Standard Deviation