anti lrrtm2 antibody  (Alomone Labs)

Journal: eLife

Article Title: Distinct but overlapping roles of LRRTM1 and LRRTM2 in developing and mature hippocampal circuits

doi: 10.7554/eLife.64742

Figure Lengend Snippet: Region specific injection in CA1 of dorsal hippocampus with AAV8-hSYN-Cre-eGFP of P0 LRRTM1/2 floxed/floxed mice to obtain a knockout of LRRTM1 and LRRTM2 in CA1 pyramidal neurons. Scale bar is 200 µm.

Article Snippet: The sections were washed with TBSTr (50 mM Tris, pH 7.4, 1.5% NaCl and 0.3% TritonX-100) for 20 min. For immunofluorescence, the slices were then incubated in primary antibodies (LRRTM1 rabbit; Alomone Labs; ANR-141) and (anti-LRRTM2 antibody (510KSCN), described in ) diluted in TBSTr containing 10% normal goat serum for 18–24 hr at 4 °C.

Techniques: Injection, Knock-Out

Journal: eLife

Article Title: Distinct but overlapping roles of LRRTM1 and LRRTM2 in developing and mature hippocampal circuits

doi: 10.7554/eLife.64742

Figure Lengend Snippet: ( A ) Representative mEPSC recordings from control and P0 LRRTM1/2-cDKO hippocampal CA1 pyramidal neurons. ( B and C ) Cumulative distributions of mEPSC inter-event intervals ( B ) and amplitudes ( C ) in control and P0-CA1-LRRTM1/2-cDKO neurons. Insets display mean ± SEM for mEPSC frequency ( B ) and amplitude ( C ) in CA1 pyramidal cells, respectively. Double knockout of Lrrtm1 and Lrrtm2 at P0 decreased mEPSC frequency and amplitude (Student’s t-test, *p=0.0157 for frequency and **p=0.0026 for amplitude, n=12–13 per group, three mice each). Figure 5—source data 1. source data for 5B and C.

Article Snippet: The sections were washed with TBSTr (50 mM Tris, pH 7.4, 1.5% NaCl and 0.3% TritonX-100) for 20 min. For immunofluorescence, the slices were then incubated in primary antibodies (LRRTM1 rabbit; Alomone Labs; ANR-141) and (anti-LRRTM2 antibody (510KSCN), described in ) diluted in TBSTr containing 10% normal goat serum for 18–24 hr at 4 °C.

Techniques: Double Knockout

Journal: eLife

Article Title: Distinct but overlapping roles of LRRTM1 and LRRTM2 in developing and mature hippocampal circuits

doi: 10.7554/eLife.64742

Figure Lengend Snippet: Representative images of LRRTM1 ( A ) and LRRTM2 ( B ) in the dorsal hippocampus showing their expression in the CA1 dendritic layers. ( A ) LRRTM1 is preferentially expressed in stratum radiatum (SR, top). AAV8-hSYN-Cre-GFP expression in the CA1 of dorsal hippocampus leads to reduced levels of LRRTM1 in the SR (*, bottom). ( B ) LRRTM2 is preferentially expressed in stratum lacunosum moleculare (SLM) of the CA1 and the outer molecular layer of dentate gyrus (top). AAV8-hSYN-Cre-GFP expression in the CA1 of dorsal hippocampus leads to reduced levels of LRRTM2 in the SLM (**) but not in the untargeted dentate gyrus OLM (control). Note that only native GFP is captured in ( A ) and ( B ). Some of the native GFP fluorescence was not captured due to low intensity exposure. Scale bar is 200 µm for all panels except GFP panel for ( A ) which is 100 µm.

Article Snippet: The sections were washed with TBSTr (50 mM Tris, pH 7.4, 1.5% NaCl and 0.3% TritonX-100) for 20 min. For immunofluorescence, the slices were then incubated in primary antibodies (LRRTM1 rabbit; Alomone Labs; ANR-141) and (anti-LRRTM2 antibody (510KSCN), described in ) diluted in TBSTr containing 10% normal goat serum for 18–24 hr at 4 °C.

Techniques: Expressing, Fluorescence