annexin v fitc pi cell apoptosis detection kit  (Beyotime)

Journal: International Journal of Pharmaceutics: X

Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition

doi: 10.1016/j.ijpx.2026.100489

Figure Lengend Snippet: In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

Article Snippet: After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis.

Techniques: In Vitro, Drug discovery, Incubation, MTT Assay, Flow Cytometry, Staining, Generated

Journal: International Journal of Nanomedicine

Article Title: Spatholobus suberectus Stem-Derived Extracellular Vesicle-Like Particles Attenuate Glucocorticoid-Induced Osteoporosis via NRF2/HO-1 Signaling

doi: 10.2147/IJN.S585928

Figure Lengend Snippet: SS-EVLP attenuates dexamethasone-induced loss of viability and apoptosis in MC3T3-E1 cells. ( a and d ) Effects of different concentrations of SS-EVLP on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( b and e ) Effects of different concentrations of dexamethasone (DEX) on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( c and f ) SS-EVLP-mediated restoration of cell viability in MC3T3-E1 cells exposed to 100 μM DEX at 24 and 48 h, respectively. ( g ) Representative flow cytometric plots of Annexin V-FITC/PI double staining in the indicated groups. ( h ) Quantification of apoptotic cells. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Cell Counting Kit-8 (CCK-8) and Annexin V-FITC/PI apoptosis detection kits were purchased from Beyotime (Shanghai, China).

Techniques: Double Staining

Journal: Journal of Neuroinflammation

Article Title: DNA methylation regulation of CYP450-lipid metabolism by high-altitude hypoxia: linking neuroinflammation to cognitive impairment

doi: 10.1186/s12974-026-03775-6

Figure Lengend Snippet: Effects of hypoxia on astrocytes. A Effect of hypoxia on astrocyte survival. B Effect of hypoxia on inflammatory factor expression. C Effects of hypoxia on Aβ and p-Tau expression. D Effect of hypoxia on apoptosis. E Effect of hypoxia on CYP450 mRNA expression. F Effect of hypoxia on CYP450 protein expression. ( n = 3. Data are shown as the mean ± SD. Statistical analyses were performed with one-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05 vs. normoxia group)

Article Snippet: An Annexin V-FITC/PI cell apoptosis detection kit (Cat: G1511) was purchased from Servicebio (Wuhan, China).

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: DNA methylation regulation of CYP450-lipid metabolism by high-altitude hypoxia: linking neuroinflammation to cognitive impairment

doi: 10.1186/s12974-026-03775-6

Figure Lengend Snippet: The effect of CYP450 inhibition on astrocytes under hypoxic conditions. ( A ) Reproductive toxicity testing of MS-PPOH and 17-ODYA on astrocytes. ( B ) Changes in CYP450 mRNA expression after CYP450 inhibition. ( C ) Changes in CYP450 protein expression after CYP450 inhibition. ( D ) Changes in inflammatory factor expression after CYP450 inhibition. ( E ) Immunofluorescence staining of Aβ, p-Tau, GFAP, and ROS expression following CYP450 inhibition (400×; green: Aβ/p-Tau/GFAP/ROS; blue: DAPI). ( F ) Western blot analysis of Aβ, p-Tau, and total Tau protein expression after CYP450 inhibition. ( G ) Changes in apoptosis after CYP450 inhibition. ( n = 3. Data are shown as the mean ± SD. Statistical analyses were performed with two-way ANOVA with the Bonferroni multiple comparisons test. Significance: * P < 0.05)

Article Snippet: An Annexin V-FITC/PI cell apoptosis detection kit (Cat: G1511) was purchased from Servicebio (Wuhan, China).

Techniques: Inhibition, Expressing, Immunofluorescence, Staining, Western Blot

Journal: Journal of Neuroinflammation

Article Title: DNA methylation regulation of CYP450-lipid metabolism by high-altitude hypoxia: linking neuroinflammation to cognitive impairment

doi: 10.1186/s12974-026-03775-6

Figure Lengend Snippet: NF-κB signaling pathway mediates the neuroprotective effects of CYP450 epoxygenase under hypoxia conditions. ( A ) Changes in inflammatory factor expression after CYP450 and NF-κB inhibition. ( B ) Changes in Aβ and p-Tau protein expression after CYP450 and NF-κB inhibition. ( C ) Changes in apoptosis after CYP450 and NF-κB inhibition. ( D ) Changes in GFAP and ROS expression after CYP450 and NF-κB inhibition. (400×; green: GFAP/ROS; blue: DAPI). ( n = 3. Data are shown as the mean ± SD. Statistical analyses were performed with two-way ANOVA with the Bonferroni multiple comparisons test. Significance: * P < 0.05)

Article Snippet: An Annexin V-FITC/PI cell apoptosis detection kit (Cat: G1511) was purchased from Servicebio (Wuhan, China).

Techniques: Expressing, Inhibition