Journal: Oncology Letters
Article Title: Targeting ETHE1 inhibits tumorigenesis in vitro and in vivo by preventing aerobic glycolysis in gastric adenocarcinoma cells
doi: 10.3892/ol.2025.15032
Figure Lengend Snippet: BALB/c nude mice received subcutaneous injections of stabilized infected NCI-N87 cells to induce tumor formation in vivo . (A) Tumor tissue images, tumor growth curve and tumor weight. (B) Representative immunohistochemical staining images of Ki67 and ETHE1. (C) Apoptosis in gastric adenocarcinoma tissue was detected by TUNEL staining. Scale bar, 100 µm. *P<0.05, **P<0.01 and ***P<0.001 vs. Ctrl. Ctrl, control; ETHEI, ethylmalonic encephalopathy protein 1; sh, short hairpin.
Article Snippet: Cell cycle distribution and apoptosis were analyzed using a flow cytometer (NovoCyte-D2060R; Agilent Technologies, Inc.), and a Cell Cycle and Apoptosis Analysis Kit (BioSharp Life Sciences) or an Annexin V-APC/PI double staining apoptosis detection kit (Nanjing KeyGen Biotech Co., Ltd.), respectively, according to the manufacturers' protocols.
Techniques: Infection, In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Control