annexin v apc pi double staining apoptosis detection kit  (Keygen Biotech)

Journal: Frontiers in Oncology

Article Title: ITPKA suppresses glioma progression and predicts patient prognosis

doi: 10.3389/fonc.2026.1802857

Figure Lengend Snippet: Effect of ITPKA overexpression on the functional characteristics of GBM cells. Flow cytometry analysis (A, B) shows the effect of upregulated ITPKA expression on the cell cycle progression of U251-MG and T98G cells; (C, D) evaluates the effect of upregulated ITPKA expression on the apoptosis level of the two indicated cell lines. All experimental data are presented as mean ± standard deviation (SD) from six independent repeated experiments, and Student’s t-test was used for statistical analysis. Data annotation: ns: not significant; *p < 0.05, ****p < 0.0001.

Article Snippet: A total of 5×10 5 stably transfected U251-MG and T98G cells were centrifuged, after which apoptosis detection was performed using the Annexin V-APC/PI Double Staining Apoptosis Detection Kit (KGA1107-100; Keygen Biotech, Nanjing, China).

Techniques: Over Expression, Functional Assay, Flow Cytometry, Expressing, Standard Deviation

Journal: Frontiers in Oncology

Article Title: ITPKA suppresses glioma progression and predicts patient prognosis

doi: 10.3389/fonc.2026.1802857

Figure Lengend Snippet: Effect of ITPKA knockdown on the functional characteristics of GBM cells. Flow cytometry analysis (A, B) shows the effect of downregulated ITPKA expression on the cell cycle progression of U251-MG and T98G cells; (C, D) evaluates the effect of downregulated ITPKA expression on the apoptosis level of the two indicated cell lines. All experimental data are presented as mean ± standard deviation (SD) from six independent repeated experiments, and Student’s t-test was used for statistical analysis. Data annotation: ns: not significant; *p < 0.05, **p < 0.01, ****p < 0.0001.

Article Snippet: A total of 5×10 5 stably transfected U251-MG and T98G cells were centrifuged, after which apoptosis detection was performed using the Annexin V-APC/PI Double Staining Apoptosis Detection Kit (KGA1107-100; Keygen Biotech, Nanjing, China).

Techniques: Knockdown, Functional Assay, Flow Cytometry, Expressing, Standard Deviation

Journal: Oncology Letters

Article Title: Targeting ETHE1 inhibits tumorigenesis in vitro and in vivo by preventing aerobic glycolysis in gastric adenocarcinoma cells

doi: 10.3892/ol.2025.15032

Figure Lengend Snippet: ETHE1 knockdown promotes CAG cell apoptosis. (A) Flow cytometric analysis of apoptosis. (B) Apoptosis of gastric adenocarcinoma cells was detected by TUNEL staining. Scale bar, 50 µm. (C) Caspase-3 and (D) caspase-9 activity was detected using kits. **P<0.01, ***P<0.001. Ctrl, control; sh, short hairpin.

Article Snippet: Cell cycle distribution and apoptosis were analyzed using a flow cytometer (NovoCyte-D2060R; Agilent Technologies, Inc.), and a Cell Cycle and Apoptosis Analysis Kit (BioSharp Life Sciences) or an Annexin V-APC/PI double staining apoptosis detection kit (Nanjing KeyGen Biotech Co., Ltd.), respectively, according to the manufacturers' protocols.

Techniques: Knockdown, TUNEL Assay, Staining, Activity Assay, Control

Journal: Oncology Letters

Article Title: Targeting ETHE1 inhibits tumorigenesis in vitro and in vivo by preventing aerobic glycolysis in gastric adenocarcinoma cells

doi: 10.3892/ol.2025.15032

Figure Lengend Snippet: BALB/c nude mice received subcutaneous injections of stabilized infected NCI-N87 cells to induce tumor formation in vivo . (A) Tumor tissue images, tumor growth curve and tumor weight. (B) Representative immunohistochemical staining images of Ki67 and ETHE1. (C) Apoptosis in gastric adenocarcinoma tissue was detected by TUNEL staining. Scale bar, 100 µm. *P<0.05, **P<0.01 and ***P<0.001 vs. Ctrl. Ctrl, control; ETHEI, ethylmalonic encephalopathy protein 1; sh, short hairpin.

Article Snippet: Cell cycle distribution and apoptosis were analyzed using a flow cytometer (NovoCyte-D2060R; Agilent Technologies, Inc.), and a Cell Cycle and Apoptosis Analysis Kit (BioSharp Life Sciences) or an Annexin V-APC/PI double staining apoptosis detection kit (Nanjing KeyGen Biotech Co., Ltd.), respectively, according to the manufacturers' protocols.

Techniques: Infection, In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Control

Journal: Frontiers in Immunology

Article Title: Deciphering colorectal cancer radioresistance and immune microrenvironment: unraveling the role of EIF5A through single-cell RNA sequencing and machine learning

doi: 10.3389/fimmu.2024.1466226

Figure Lengend Snippet: In vitro validation of radiation resistance by EIF5A. (A, B) The transfection efficiency of siEIF5A in HCT116 cell line, which was examined by western blot and qRT-PCR. (C) Representative wound healing images of migration after knocking down EIF5A. (D) Representative transwell images of migration and invasion after knocking down EIF5A. (E) CCK-8 assay to analyze the effect of radiation on cell proliferation of the irradiated and non-irradiated groups after knocking down EIF5A. (F) Representative colony formation images of the irradiated and non-irradiated groups after knocking down EIF5A. (G) Representative ROS production images of the irradiated and non-irradiated groups after knocking down EIF5A. (H) Flow cytometry assay to analyze the cell apoptosis changes in irradiated and non-irradiated groups after knocking down EIF5A. (I) Calcein-AM/PI staining assay and EdU incorporation assay to detected the cell death and proliferation in irradiated and non-irradiated groups after knocking down EIF5A. qRT-PCR, Quantitative reverse transcription polymerase chain reaction; CCK-8, Cell counting kit-8; ROS, Reactive oxygen species; EdU, 5-Ethynyl-2’-deoxyuridine; NC, Negative control; KD, Knock down; IR, Irradiation; ns, Non-statistics significance;*: P < 0.05; **: P < 0.01; ***: P < 0.001.

Article Snippet: All groups’ cells were analyzed with an annexin V-APC/PI double staining apoptosis detection kit (KeyGen BioTech) according to the manufacturer’s instructions, respectively.

Techniques: In Vitro, Biomarker Discovery, Transfection, Western Blot, Quantitative RT-PCR, Migration, CCK-8 Assay, Irradiation, Flow Cytometry, Staining, Reverse Transcription, Polymerase Chain Reaction, Cell Counting, Negative Control, Knockdown

Journal: Journal of Translational Medicine

Article Title: Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas

doi: 10.1186/s12967-024-05151-5

Figure Lengend Snippet: Isi BCL-2 induces apoptosis and activates the RIG-I signaling pathway in glioma cells. A . Flow cytometry analysis of apoptosis in U251 cells 48 h after transfection with indicated RNAs. B . Western blotting analysis of protein levels of BCL-2, BAX, cytochrome C, cleaved caspase-3, cleaved caspase-9, and PARP in U251 cells 48 h after transfection with indicated RNAs. C . Quantitative analysis of RIG-I mRNA levels using qRT-PCR after a 48-h treatment with indicated RNAs. D . Western blotting analysis of protein levels of RIG-I, MAVS, IRF3, and P-IRF3 in U87 and U251 cells 48 h after transfection with indicated RNAs. E . Confocal fluorescence microscopy images of RIG-I and MAVS levels. Scale bar: 50 μm. Data are presented as mean ± standard deviation ( n = 3). * p < 0.05 vs. control

Article Snippet: Subsequently, cells were harvested and stained with Annexin V/PI using the Annexin V/PI Double Stain Apoptosis Detection Kit (Elabscience, Wuhan, China) following the manufacturer’s protocols.

Techniques: Flow Cytometry, Transfection, Western Blot, Quantitative RT-PCR, Fluorescence, Microscopy, Standard Deviation, Control

Journal: Journal of Translational Medicine

Article Title: Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas

doi: 10.1186/s12967-024-05151-5

Figure Lengend Snippet: Effects of isi BCL-2 on cell viability, apoptosis, cytokine release, and upregulation of MHC-I require RIG-I activation. A . Assessment of cell viability and apoptosis in U251 cells using CCK-8 and flow cytometry assays. B . Quantification of RIG-I , IFN-β , and CXCL10 mRNA levels by qRT-PCR. C . Quantitative analysis of IFN-β and CXCL10 secretion levels using ELISA. D . Western blotting analysis of protein levels of RIG-I, MAVS, and P-IRF3. E . Flow cytometry analysis of MHC-I levels. Data are presented as mean ± standard deviation ( n = 3). * p < 0.05 vs. control

Article Snippet: Subsequently, cells were harvested and stained with Annexin V/PI using the Annexin V/PI Double Stain Apoptosis Detection Kit (Elabscience, Wuhan, China) following the manufacturer’s protocols.

Techniques: Activation Assay, CCK-8 Assay, Flow Cytometry, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Standard Deviation, Control

Journal: Journal of Translational Medicine

Article Title: Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas

doi: 10.1186/s12967-024-05151-5

Figure Lengend Snippet: isi BCL-2 Exerts antitumor effects in vivo. A . Establishment of a xenograft nude mice model bearing human glioma U251 cells, with subsequent treatment using a specified amount of isi BCL-2 . B . Removal, photography, and weighing of tumors after 22 days of RNA treatment. Tumor volume and mouse body weight were monitored every two days. C . Immunohistochemical (IHC) analysis, hematoxylin and eosin (H&E) staining, and TUNEL assay of tumors excised from U251 tumor-bearing mice. BCL-2 and Ki67 protein levels in the tumor tissue assessed using IHC staining. The apoptosis rate in each group was determined using H&E staining and TUNEL assay. Scale bar: 40 μm Data are presented as mean ± standard deviation ( n = 3). * p < 0.05 vs. PBS (control)

Article Snippet: Subsequently, cells were harvested and stained with Annexin V/PI using the Annexin V/PI Double Stain Apoptosis Detection Kit (Elabscience, Wuhan, China) following the manufacturer’s protocols.

Techniques: In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Immunohistochemistry, Standard Deviation, Control