annexin v apc pi apoptosis detection kit  (Elabscience Biotechnology)

Journal: Frontiers in Oncology

Article Title: ITPKA suppresses glioma progression and predicts patient prognosis

doi: 10.3389/fonc.2026.1802857

Figure Lengend Snippet: Effect of ITPKA overexpression on the functional characteristics of GBM cells. Flow cytometry analysis (A, B) shows the effect of upregulated ITPKA expression on the cell cycle progression of U251-MG and T98G cells; (C, D) evaluates the effect of upregulated ITPKA expression on the apoptosis level of the two indicated cell lines. All experimental data are presented as mean ± standard deviation (SD) from six independent repeated experiments, and Student’s t-test was used for statistical analysis. Data annotation: ns: not significant; *p < 0.05, ****p < 0.0001.

Article Snippet: A total of 5×10 5 stably transfected U251-MG and T98G cells were centrifuged, after which apoptosis detection was performed using the Annexin V-APC/PI Double Staining Apoptosis Detection Kit (KGA1107-100; Keygen Biotech, Nanjing, China).

Techniques: Over Expression, Functional Assay, Flow Cytometry, Expressing, Standard Deviation

Journal: Frontiers in Oncology

Article Title: ITPKA suppresses glioma progression and predicts patient prognosis

doi: 10.3389/fonc.2026.1802857

Figure Lengend Snippet: Effect of ITPKA knockdown on the functional characteristics of GBM cells. Flow cytometry analysis (A, B) shows the effect of downregulated ITPKA expression on the cell cycle progression of U251-MG and T98G cells; (C, D) evaluates the effect of downregulated ITPKA expression on the apoptosis level of the two indicated cell lines. All experimental data are presented as mean ± standard deviation (SD) from six independent repeated experiments, and Student’s t-test was used for statistical analysis. Data annotation: ns: not significant; *p < 0.05, **p < 0.01, ****p < 0.0001.

Article Snippet: A total of 5×10 5 stably transfected U251-MG and T98G cells were centrifuged, after which apoptosis detection was performed using the Annexin V-APC/PI Double Staining Apoptosis Detection Kit (KGA1107-100; Keygen Biotech, Nanjing, China).

Techniques: Knockdown, Functional Assay, Flow Cytometry, Expressing, Standard Deviation

Journal: Cell Reports Medicine

Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells

doi: 10.1016/j.xcrm.2026.102698

Figure Lengend Snippet: The secretion of MMP2 by CAR-M2 activates the transcription factor Rxra in Cxcr2 + ECs (A) Position relationship between CXCR2 + ECs and FAP + fibroblasts was visualized by immunofluorescence staining. Blue: DAPI, green: CD31, red: CXCR2, French gray: FAP. (B) Volcano plot of differentially expressed genes before and after co-culture of CAR-M2 with FAP + fibroblasts. (C) Representative Masson and Sirius red staining images of mice kidney tissue sections following the injection of PBS into the renal subcapsule (Sham and UUO), CAR-M2-loaded HAMA-CS hydrogel injected into the renal subcapsule (CAR-M2), and PBS into the renal subcapsule of MMP2 inhibitor mice (MMP2 inhibitor). Detection at day 7. Scale bars, 100 μm. (D) Average collagen deposition density by Masson staining in (C), n = 5. (E) Average collagen deposition density (the percentage of positively stained area per HPF) by Sirius red staining in (C), n = 5. (F) Re-clustering of endothelial cells from human kidney single-cell sequencing data based on regulon activity, t-SNE (t-distributed stochastic neighbor embedding) displayed by cell type (left), and kidney condition (right). (G) SCENIC analysis of cell stability in clusters derived from regulon activity-based dimensionality reduction. (H) Ranked plot of transcription factor activity in CXCR2 + ECs. (I) Differential expression of the Rxra gene among groups in mouse single-cell transcriptome samples. (J) Changes in the endothelial transcription factor RXRA after CAR-M2 treatment and in MMP2 inhibitor-treated mice. Blue: DAPI, red: CD31, green: Rxra, yellow indicates the co-labeling of CD31 with Rxra, Scale bars, 25 μm. Arrowheads indicate the double positive cells. (K) Statistical analysis of the number of CD31 + Rxra + cells in (J), n = 5. (L) Cell co-culture protocol to verify that CAR-M2 activates the transcription factor Rxra by secreted MMP2. (M) After cell co-culture using protocol in (L), the lentiviral constructs Cxcr2 + ECs were collected to detect the changes in their Rxra and Cxcr2 expression levels. (N) Average collagen deposition density by Masson staining, mice kidney tissue sections following the injection of PBS into the renal subcapsule (Control), CAR-M2-loaded HAMA-CS hydrogel injected into the renal subcapsule (CAR-M2), and PBS into the renal subcapsule of Rxra agonist and inhibitor mice (Rxra agonist and Rxra inhibitor). Detection at day 14. n = 5. (O) Average collagen deposition density by Sirius red staining, mice kidney tissue sections following the injection of PBS into the renal subcapsule (Control), CAR-M2-loaded HAMA-CS hydrogel injected into the renal subcapsule (CAR-M2), and PBS into the renal subcapsule of Rxra agonist and inhibitor mice (Rxra agonist and Rxra inhibitor). Detection at day 14. n = 5. (P) Mitochondrial autophagy-related gene score in human normal and CKD groups. (Q) Apoptotic-related gene score in human normal and CKD groups. (R) Mitochondrial autophagy-related gene score in mice Sham, UUO, and CAR-M2 groups. (S) Apoptotic-related gene score in mice Sham, UUO, and CAR-M2 groups. (T) The changes in the expression levels of autophagy-related protein PINK1 and apoptosis-related protein active caspase-3. (U) The cell apoptosis rate after the aforementioned (T) co-culture was detected by flow cytometry, and the proportion of Annexin-APC positive cells was counted as the cell apoptosis rate. The detection time was 6 h after co-culture. n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant (one-way ANOVA). See also .

Article Snippet: Annexin V-APC / PI Apoptosis Detection Kit , Elabscience , Cat#E-CK-A217.

Techniques: Immunofluorescence, Staining, Co-Culture Assay, Injection, Single Cell, Sequencing, Activity Assay, Derivative Assay, Quantitative Proteomics, Labeling, Construct, Expressing, Control, Flow Cytometry

Journal: Inflammation

Article Title: Red Blood Cell-Derived Exosomes Deliver Complement C5 to Exacerbate Neuroinflammation and Neuronal Injury after Intracerebral Hemorrhage

doi: 10.1007/s10753-026-02456-z

Figure Lengend Snippet: ICH-Exos increased necroptosis and M1 polarization in hemin-induced primary murine microglia. ( A ) Cell viability was determined using the CCK-8 assay. ( B ) Cell apoptosis was assessed via flow cytometry. ( C ) Western blot analysis was conducted to measure p-RIPK1, RIPK1, p-RIPK3, RIPK3, p-MLKL, and MLKL levels. ( D and E ) Flow cytometry was used to examine the proportions of CD86 + and CD163 + macrophages. ( F ) qRT-PCR analysis of iNOS, COX-2, CCL2, ARG1, CD206, and YM1 levels. ( G ) iNOS, COX-2, ARG1, and CD206 proteins levels. ( H ) ELISA was conducted to assess IL-1β, IL-6, and TNF-α levels. n = 3. * p < 0.05 vs. Control group; # p < 0.05 vs. Hemin group

Article Snippet: The cell pellet was mixed with annexin V-APC and propidium iodide (PI) from the annexin V-APC/PI cell apoptosis detection kit (KGA1030, KeyGEN BioTECH, Jiangsu, China).

Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control

Journal: Inflammation

Article Title: Red Blood Cell-Derived Exosomes Deliver Complement C5 to Exacerbate Neuroinflammation and Neuronal Injury after Intracerebral Hemorrhage

doi: 10.1007/s10753-026-02456-z

Figure Lengend Snippet: ICH-Exos increased necroptosis of macrophages/microglia and M1 polarization after ICH. ( A ) Cell apoptosis was detected by TUNEL. ( B ) p-RIPK1, RIPK1, p-RIPK3, RIPK3, p-MLKL, and MLKL protein levels. ( C and D ) Immunofluorescence was used to detect IBA1 + iNOS + and IBA1 + ARG1 + . ( E ) iNOS, COX-2, CCL2, ARG1, CD206, and YM1 mRNA levels. ( F ) iNOS, COX-2, ARG1, and CD206 levels were analyzed by western blot. ( G ) ELISA was conducted to examine IL-1β, IL-6, and TNF-α. n = 5. * p < 0.05 vs. Sham group; # p < 0.05 vs. ICH group

Article Snippet: The cell pellet was mixed with annexin V-APC and propidium iodide (PI) from the annexin V-APC/PI cell apoptosis detection kit (KGA1030, KeyGEN BioTECH, Jiangsu, China).

Techniques: TUNEL Assay, Immunofluorescence, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Inflammation

Article Title: Red Blood Cell-Derived Exosomes Deliver Complement C5 to Exacerbate Neuroinflammation and Neuronal Injury after Intracerebral Hemorrhage

doi: 10.1007/s10753-026-02456-z

Figure Lengend Snippet: ICH-Exos C5 promoted necroptosis and M1 polarization in hemin-induced microglia. ( A ) The heat map shows the differentially expressed proteins. ( B ) C5a level in brain tissue analyzed by IHC. * p < 0.05 vs. sham group. ( C ) C5a level in serum was detected by ELISA. * p < 0.05 vs. sham group. Under RIPA lysis conditions, the results of Western blot for C5a in ( D ) human RBC-Exos and ( E ) murine RBC-Exos after untreated EVs or after treatment with proteinase K (5 U/mL, 10 min) . # p < 0.05 vs. Sham-Exos/Normal-Exos group; @ p < 0.05 vs. ICH-Exos group. ( F ) C5a level in HMC3 cells was analyzed by Western blot and ELISA. ( G ) Cell viability was detected by CCK-8. ( H ) Cell apoptosis was detected by flow cytometry. ( I ) p-RIPK1, RIPK1, p-RIPK3, RIPK3, p-MLKL, and MLKL levels were detected by Western blot. ( G ) The proportions of CD86 + M1 and CD163 + M2 macrophages were detected by flow cytometry. ( K ) iNOS, COX-2, CCL2, ARG1, CD206, and YM1 levels analyzed by qRT-PCR. ( L ) iNOS, COX-2, ARG1 and CD206 levels analyzed by Western blot. (M) ELISA was used to detect IL-1β, IL-6, and TNF-α levels. n = 3. # p < 0.05 vs. Normal-Exos group; & p < 0.05 vs. ICH-Exos group

Article Snippet: The cell pellet was mixed with annexin V-APC and propidium iodide (PI) from the annexin V-APC/PI cell apoptosis detection kit (KGA1030, KeyGEN BioTECH, Jiangsu, China).

Techniques: Paraffin-embedded Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Lysis, Western Blot, CCK-8 Assay, Flow Cytometry, Quantitative RT-PCR