Journal: Heliyon

Article Title: Assessment of ELISA-based method for the routine examination of serum indoxyl sulfate in patients with chronic kidney disease

doi: 10.1016/j.heliyon.2022.e12220

Figure Lengend Snippet: The linearity, sensitivity, and repeatability of three commercial ELISA kits.

Article Snippet: The standard curves of commercial ELISA kits purchased from BlueGene Biotech (Cat. No. E01I0039, Shanghai, China), Leadgene (Cat. No. 00201, Taiwan), and Mybio-source (Cat. No. MBS019983, San Diego, CA, USA) were prepared according to the manufacturer's instructions ( ).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Assessment of ELISA-based method for the routine examination of serum indoxyl sulfate in patients with chronic kidney disease

doi: 10.1016/j.heliyon.2022.e12220

Figure Lengend Snippet: Quantification of serum IS levels using UPLC-MS/MS- and ELISA-based methodology. (A) The indoxyl sulfate potassium was prepared to concentrations of 2, 4, and 8 μmol/L and determined by commercial ELISA kits according to the manufacturer's instructions. (B) The serum IS levels in 17 clinical samples in group 1 were determined using UPLC-MS/MS and commercial ELISA kits. The data were presented as means ± SEM, and the significance of differences between the IS levels determined by UPLC-MS/MS and commercial IS ELISA kits was evaluated by Wilcoxon signed-rank test.

Article Snippet: The standard curves of commercial ELISA kits purchased from BlueGene Biotech (Cat. No. E01I0039, Shanghai, China), Leadgene (Cat. No. 00201, Taiwan), and Mybio-source (Cat. No. MBS019983, San Diego, CA, USA) were prepared according to the manufacturer's instructions ( ).

Techniques: Tandem Mass Spectroscopy, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Assessment of ELISA-based method for the routine examination of serum indoxyl sulfate in patients with chronic kidney disease

doi: 10.1016/j.heliyon.2022.e12220

Figure Lengend Snippet: Bland-Altman analysis of serum IS levels determined using UPLC-MS/MS- and ELISA-based methods. Bland-Altman plots of serum IS levels determined by UPLC-MS/MS versus those by Bluegene (A), Leadgene (B), and Mybio-source (C) ELISA kits. The data were presented as the means ± 1.96∗SD and agreements between UPLC-MS/MS- and ELISA-base methods were evaluated by one-sample t test.

Article Snippet: The standard curves of commercial ELISA kits purchased from BlueGene Biotech (Cat. No. E01I0039, Shanghai, China), Leadgene (Cat. No. 00201, Taiwan), and Mybio-source (Cat. No. MBS019983, San Diego, CA, USA) were prepared according to the manufacturer's instructions ( ).

Techniques: Tandem Mass Spectroscopy, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Assessment of ELISA-based method for the routine examination of serum indoxyl sulfate in patients with chronic kidney disease

doi: 10.1016/j.heliyon.2022.e12220

Figure Lengend Snippet: Correlation analysis of serum IS levels determined by UPLC-MS/MS and commercial ELISA kits.

Article Snippet: The standard curves of commercial ELISA kits purchased from BlueGene Biotech (Cat. No. E01I0039, Shanghai, China), Leadgene (Cat. No. 00201, Taiwan), and Mybio-source (Cat. No. MBS019983, San Diego, CA, USA) were prepared according to the manufacturer's instructions ( ).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: Assessment of ELISA-based method for the routine examination of serum indoxyl sulfate in patients with chronic kidney disease

doi: 10.1016/j.heliyon.2022.e12220

Figure Lengend Snippet: Correlation analysis of serum IS levels and renal function parameters in patients with CKD. Correlations between the eGFR and serum creatinine level (A), IS levels determined by UPLC-MS/MS and Leadgene ELISA kit (B), eGFR and UPLC-MS/MS-determined IS levels (C), eGFR and Leadgene ELISA-determined IS levels (D), serum creatinine level and UPLC-MS/MS-determined IS levels (E), and serum creatinine level and Leadgene ELI-SA-determined IS levels (F) were analyzed by Spearman's correlation coefficients.

Article Snippet: The standard curves of commercial ELISA kits purchased from BlueGene Biotech (Cat. No. E01I0039, Shanghai, China), Leadgene (Cat. No. 00201, Taiwan), and Mybio-source (Cat. No. MBS019983, San Diego, CA, USA) were prepared according to the manufacturer's instructions ( ).

Techniques: Tandem Mass Spectroscopy, Enzyme-linked Immunosorbent Assay