Journal: In Vitro Cellular & Developmental Biology. Animal
Article Title: The role of semaphorin 3A on chondrogenic differentiation
doi: 10.1007/s11626-024-00909-z
Figure Lengend Snippet: Effects of Sema3A on chondrogenic differentiation. ATDC5 cells were treated with 0, 1, 10, or 100 ng/mL Sema3A for 24 h on day 7. The gene expression levels of ( A ) COL2A1 , ( B ) SRY-box transcription factor 9 ( Sox9 ), ( C ) hyaluronan synthase 2 ( HAS2 ), ( D ) Runt-related transcription factor 2 ( Runx2 ), ( E ) Aggrecan ( ACAN ), and ( F ) COL10A1 were determined using qPCR. (* p < 0.05, ** p < 0.01, N = 16).
Article Snippet: The concentrations of Hyaluronan in the supernatant were measured using a Hyaluronan quantification kit (Cosmo Bio, Tokyo, Japan).
Techniques: Expressing