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ReproCELL sb431542
Sb431542, supplied by ReproCELL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ams%2E04-0010-10/Stemolecule+SB431542/bio_rxiv__2024__12__13__628384-291-10-11
Average 96 stars, based on 1 article reviews

sb431542 - by Bioz Stars, 2026-09

96/100 stars

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Blocking Assay:

Article Title: Microglia depletion reduces human neuronal APOE4-related pathologies in a chimeric Alzheimer's disease model.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Human Nuclear Antigen Abcam Cat# ab84680; RRID: AB_2154610 Mouse anti-Human Nuclear Antigen Millipore Sigma Cat# MAB1281; RRID:AB_94090 Rabbit anti-IBA1 Wako Cat# 019-19741 RRID:AB_839504 Goat anti-IBA1 Abcam Cat# ab5076; RRID: AB_2224402 Rabbit anti-IBA1 Abcam Cat# ab178846; RRID: AB_2636859 Rabbit anti-GFAP Agilent Dako Cat# Z0334; RRID: AB_10013382 Mouse anti-GFAP Sigma Cat# MAB3402; RRID: AB_94844 Chicken anti-MAP2 Thermo Fisher Scientific Cat# PA1-10005; RRID: AB_1076848 Mouse anti-3D6 Elan Pharmaceuticals N/A Mouse anti-AT8 Thermo Fisher Scientific Cat# MN1020; RRID: AB_223647 Rabbit anti-pTau(Ser202/Thr205) ABClonal Cat# AP0894; RRID: AB_2771603 Goat anti-APOE Millipore Sigma Cat# 178479; RRID: AB_10682965 Rabbit anti-OLIG2 Millipore Sigma Cat# AB9610; RRID: AB_570666 Mouse anti-Synaptophysin Fisher Scientific Cat# 50-134-87; RRID: Not registered Guinea pig anti-NeuN Millipore Sigma Cat# ABN90; RRID: AB_11205592 Mouse anti-PHF1 Peter Davies Lab N/A Hamster anti-CD3 Bio-Rad Cat# MCA2690; RRID: AB_905951 Rabbit anti-P2RY12 Millipore Sigma Cat# HPA014518; RRID: AB_2669027 Mouse anti-CD68 Thermofisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) BD Biosciences Cat# 553141; RRID: AB_394656 CD45 Monoclonal Antibody (30-F11), PE-Cyanine7 (100ug) ThermoFisher Scientific Cat# 25-0451-82; RRID: AB_2734986 Brilliant Violet 421 anti-mouse/human CD11b Antibody (125uL) BioLegend Cat# 101236; RRID: AB_11203704 PE anti-mouse CD115 (CSF-1R) Antibody Biolegend Cat# 135505; RRID: AB_1937254 Donkey anti-mouse 488nm Thermo Fisher Scientific Cat# A-21202; RRID:AB_141607 Donkey anti-rabbit 488nm Thermo Fisher Scientific Cat# A-21206; RRID:AB_2535792 Donkey anti-goat 488nm Abcam Cat# ab150129; RRID: AB_2687506 Donkey anti-mouse 594nm Thermo Fisher Scientific Cat# R37115; RRID:AB_2556543 Donkey anti-rabbit 594nm Thermo Fisher Scientific Cat#A-21207; RRID:AB_141637 Chemicals, Peptides, Disposable Materials, and Recombinant Proteins Mouse-on-Mouse Blocking Reagent Vector Labs Cat# MKB-2213; Normal Donkey Serum Jackson Labs Cat# 017-000-121 Triton-X Millipore Sigma Cat# T8787-250mL Tween-20 Millipore Sigma Cat# P2287-500mL Accutase Millipore Sigma Cat# SCR005 NEAA GIBCO Cat# 11140-050 Glutamax GIBCO Cat# 35050-061 Pen/Strep GIBCO Cat# 15140-122 SB Stemgent Cat# 04-0010-10 LDN Stemgent Cat# 04-0074 Rock Inhibitor Tocris Cat# 1254 (Continued on next page) e1 Cell Stem Cell 32, 1–19.e1–e7, January 2, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER bFGF Peprotech Cat# 100-18C EGF Peprotech Cat# AF-100-15 Poly-L-Lysine Sigma Cat# 4707 PBS Thermo Fisher Scientific Cat# 14190250 Laminin GIBCO Cat# 23017-015 DMEM/F12 GIBCO Cat# 11330-032 Neurobasal GIBCO Cat# 21103-049 DAPT Tocris Cat# 2634 BDNF Peprotech Cat# 450-02 GDNF Peprotech Cat# 450-10 Matrigel hESC Qualified Corning Cat# 354277 Matrigel Growth Factor Reduced Corning Cat# 354230 N2 Supplement Thermo Fisher Scientific Cat# 17502048 mTESR Stemcell technologies Cat# 85850 B27 Supplement Thermo Fisher Scientific Cat# 17504044 STEMdiff Neural Progenitor Medium Stem Cell Technologies Cat# 05833 Ketamine Henry Schein Cat# 1049007 Xylazine (Anased) Henry Schein Cat# 1311139 Isofluorane Henry Schein Cat# 029405 Buprenorphine Henry Schein Cat# 055175 Ketofen Henry Schein Cat# 005487 Avertin (2,2,2-Tribromoethanol) Millipore Sigma Cat# T48402 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 InVivoMab anti-mouse CD40L (CD154) Clone MR-1 BioXCell Cat# BE0017-1 InVivoMab anti-mouse CTLA-4 (CD152) Clone 9D9 BioXCell Cat# BE0164 InVivoMab anti-mouse LFA-1a (CD11a) Clone M17/4 BioXcell Cat# BE0006 Collagenase D Sigma-Aldrich Cat# 11088866001 Debris Removal Solution Miltenyi Biotec Cat# 130-109-398 Critical Commercial Assays Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics Cat#1000268 Deposited data scRNA-seq of microglia from E3KI, E4KI, hE3-E3KI, hE4-E4KI, hEKO-E4KI mice This paper GEO: GSE248020 Experimental Models: Cell Lines Human: ApoE4/4 iPSC line Wang et al.12 N/A Human: Isogenic ApoE3/3 iPSC line Wang et al.12 N/A Human: ApoE-KO iPSC line Wang et al.12 N/A Experimental Models: Organisms/Strains Mouse: ApoE4-KI: B6.129P2-Apoetm3(APOE*4)Mae N8 Taconic Cat#1549-M Mouse: ApoE3-KI: B6.129P2-Apoetm2(APOE*3)Mae N8 Taconic Cat#1548-M Software and Algorithms FlowJo v10.9.0 BD Biosciences https://www.flowjo.com/solutions/flowjo Fiji v2.14 Schindelin et al.129 https://imagej.net/software/fiji/downloads (Continued on next page) Cell Stem Cell 32, 1–19.e1–e7, January 2, 2025 e2

Clinical Proteomics:

Recombinant:

Article Title: Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines
Article Snippet: For stage 1, the bEpiSCs were dissociated into small pieces, and cultured in MDBM supplemented with 1% B27 supplement, 3 μM CHIR99021, and 2 μM SB431542 (Selleckchem, S1067) maintained for 3 days. .. In stage 2, the culture medium was replaced with a combination of 3 μM CHIR99021, 2 μM SB431542, 0.5 μM LDN193189 (Stemgent, 04–0074), and 20 ng/mL recombinant human FGF-basic (154 a.a.) from day 4 to day 6. .. For stage 3, the culture was replaced with 10 ng/mL HGF (Peprotech, 100-39H), 10 ng/mL IGF-1 (Peprotech, 100–11), 20 ng/mL recombinant human FGF-basic (154 a.a.), and 0.5 μM LDN193189 for 2 days.

other:

Article Title: Reconstitution of Human Brain Cell Diversity in Organoids via Four Protocols
Article Snippet: Days 0, 2:150 μl NI supplemented with RevitaCell, 200 ng ml-1 Noggin (R&D Systems, cat. no. 6057), 10 μM SB431542 (Stemgent, cat. no. 04-0010-10) and 0.8 μM CHIR.

Concentration Assay:

Article Title: Synchronous 3D patterning of diverse CNS progenitors generates motor neurons of broad axial identity
Article Snippet: .. Concentration of small molecules for SCO protocols used: 10 μM SB431542 (Stemgent), 1μM LDN193189 (Stemgent), 3μM CHIRON99021 (Tocris), 1μM RXR inhibitor UVI 3003 (Tocris), 1μM RAR inhibitor AGN 193109 (Tocris), 1μM, 50nM or 25nM RA (Sigma-Aldrich), 200ng/ml FGF8 (Peprotech), 10μM Notch-inhibitor DAPT (Tocris), 120ng/ml FGF2 (Qkine), 1μM Smoothened Agonist SAG (Merck), 50ng/ml GDF11 (Peprotech), 10ng/ml BDNF (Qkine), 10ng/ml GDNF (Qkine). .. Neural Induction Media (NIM) for SCO culture contained 477.5 ml Advanced DMEM and 477.5 ml Neurobasal Media (both from Life Technologies), 10 ml Penicillin-Streptamycin and 10 ml B27 supplement with Vitamin A (Thermo Scientific), 10 ml GlutaMax (Gibco), 1.82 ml ß-Mercaptoethanol (Sigma-Aldrich), 5 ml N2 supplement (Thermo Scientific), 8 ml 20% glucose in 1xDPBS (sterile filtered from Sigma Aldrich), 200 μl 5,000x Ascorbic Acid (StemCell), and 1 ml 1,000x Antioxidant Supplement (Thermo Scientific).

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