anti cholinergic muscarinic receptor 1  (Alomone Labs)

Journal: PLoS ONE

Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

doi: 10.1371/journal.pone.0226954

Figure Lengend Snippet: (A) Calretinin (CR) staining in a parasagittal slice of gerbil cochlear nucleus. The filled arrows point to strong calretinin staining corresponding to big auditory nerve fiber terminals, the endbulb of Held. Scale: 100 μm. (B) M1 muscarinic staining was found on all AVCN cell bodies. (C) Merged image of A and B. Filled arrows show M1-positive SBCs. Empty arrows show M1-positive cells which are not SBCs. (D) Digital magnification of the white square in C. Scale: 50 μm. (E) Calretinin staining in a parasagittal slice of gerbil cochlear nucleus in which endbulb of Held could be identified. Scale: 100 μm. (F) M2 muscarinic staining was mostly present in the anterior part of AVCN, around SBCs. Filled arrowheads indicate sparse but strong M2 staining close to cell bodies. (G) Merged image of E and F. (H) Digital magnification of the white square in G. Empty arrowheads point to M2-positive puncta on SBC cell bodies. Scale: 50 μm (magnification: 63x). D = dorsal; P = posterior; NR = nerve root.

Article Snippet: The primary antibodies used were: goat anti-calretinin antibody (1/500, Merck Millipore, Germany), rabbit anti-M1 muscarinic receptor (443–458) antibody (1/200, #AMR-010, RRID: AB_2340994, Alomone labs, Israel) and rabbit anti-M2 muscarinic receptor antibody (1/200, #AMR-002, RRID: AB_2039995, Alomone labs, Israel).

Techniques: Staining

Journal: PLoS ONE

Article Title: Muscarinic modulation of M and h currents in gerbil spherical bushy cells

doi: 10.1371/journal.pone.0226954

Figure Lengend Snippet: On one hand, acetylcholine (Ach) binds the M1/M3 muscarinic receptor which induces the activation of the phospholipase C (PLC) via the Gα q/11 protein. The activated PLC depletes the phosphatidylinositol biphosphate (PIP 2 ) into diacylglycerol (DAG) and inositol triphosphate (IP 3 ). IP 3 concentration increase releases calcium from the internal cell storage. Directly or indirectly, one or several of these 2 nd messengers trigger the closure of Kv7 channels thereby depolarizing the cell membrane. On the other hand, acetylcholine may bind the M2 muscarinic receptor inhibiting the adenylate cyclase (AC) via the Gα i protein which induces a reduction of cyclic AMP. Thus, for the same membrane potential, more HCN channels are closed inducing the hyperpolarization of the cell. Cell membrane and ion channels were designed by “ Servier Medical Art ” ( https://smart.servier.com ) provided by Les Laboratoires Servier ( https://servier.com/en ), licensed under Creative Commons Attribution 3.0 Unported License .

Article Snippet: The primary antibodies used were: goat anti-calretinin antibody (1/500, Merck Millipore, Germany), rabbit anti-M1 muscarinic receptor (443–458) antibody (1/200, #AMR-010, RRID: AB_2340994, Alomone labs, Israel) and rabbit anti-M2 muscarinic receptor antibody (1/200, #AMR-002, RRID: AB_2039995, Alomone labs, Israel).

Techniques: Activation Assay, Concentration Assay

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

Article Title: Cholinergic activation of enteric glia is a physiological mechanism that contributes to the regulation of gastrointestinal motility

doi: 10.1152/ajpgi.00155.2018

Figure Lengend Snippet: Primary antibodies used

Article Snippet: Rabbit anti-M1 muscarinic receptor , Alomone, Jerusalem, Israel , 1:200 , AMR-010 , AB_2340994.

Techniques:

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

Article Title: Cholinergic activation of enteric glia is a physiological mechanism that contributes to the regulation of gastrointestinal motility

doi: 10.1152/ajpgi.00155.2018

Figure Lengend Snippet: Primary antibodies used

Article Snippet: Fluorescent labeling was visualized by confocal imaging through the Plan-Apochromat 60X oil immersion objective (1.42 numerical aperture) of an inverted Olympus Fluoview FV-1000 microscope (Olympus, Center Valley, PA). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Source Dilution Cat. No. Resource ID No. Chicken anti-GFAP Abcam, Cambridge, MA 1:1,000 AB-4674 AB_304558 Biotinylated mouse anti-human HuC/D Invitrogen, Carlsbad, CA 1:200 {"type":"entrez-nucleotide","attrs":{"text":"A21272","term_id":"514140","term_text":"A21272"}} A21272 AB_2535822 Rabbit anti-M1 muscarinic receptor Alomone, Jerusalem, Israel 1:200 AMR-010 AB_2340994 Rabbit anti-M2 muscarinic receptor Alomone 1:200 AMR-002 AB_2039995 Rabbit anti-M3 muscarinic receptor Alomone 1:200 AMR-006 AB_2039997 Rabbit anti-M4 muscarinic receptor Alomone 1:200 AMR-004 AB_11219338 Rabbit anti-M5 muscarinic receptor Alomone 1:200 AMR-005 AB_10658757 Open in a separate window GFAP, glial fibrillary acidic protein.

Techniques: