amplitaq gold360  (Thermo Fisher)


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    Name:
    AmpliTaq Gold DNA Polymerase
    Description:
    AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
    Catalog Number:
    4311806
    Price:
    None
    Applications:
    Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher amplitaq gold360
    AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
    https://www.bioz.com/result/amplitaq gold360/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold360 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Filtration:

    Article Title: Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples
    Article Snippet: .. Further optimization experiments and reduction in reaction volume combined with a previous filtration protocol ( ) resulted in the final reaction conditions of 5 µl of total reaction volume consisting of 1× PCR buffer (Qiagen), 10 nM fluorecene (Bio-Rad), 3.5 mM total MgCl2 (Qiagen), 150 nM of each V6 primer (IDT), 50 nM temperature calibrator sequences (IDT), 1× EvaGreen (Sigma), 200 µM dNTP (Invitrogen), 0.05 U/µl AmpliTaq Gold Low DNA Taq polymerase (Invitrogen), 2 µl of genomic DNA (gDNA) dilution and ultrapure water (Quality Biological), with a 20 µl of overlay of high-quality DNase-free mineral oil (Sigma). .. For each bacterial gDNA template , standard 10-fold dilutions of template gDNA in ultrapure DNase-treated water ranging from approximately 106 to 0 copies per PCR reaction were run using MyIQ qPCR (Bio-Rad) machine.

    Ancient DNA Assay:

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA
    Article Snippet: .. These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research. .. In contrast, when the oligonucleotide carrying the U was used, both polymerase reagents inserted A residues opposite the template sites carrying a U residue in all clones sequenced (data not shown).

    Real-time Polymerase Chain Reaction:

    Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶
    Article Snippet: .. Applied Biosystems' AmpliTaq Gold Taq polymerase contained in the master mix of all of our qPCR TaqMan assays did not contain any IAP sequences ( D), indicating that it was free of mouse DNA. .. When additional AmpliTaq Gold Taq polymerase was added as a template for the IAP qPCR assay, as was done with the other polymerase preparations, all reactions remained negative.

    other:

    Article Title: A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms
    Article Snippet: AmpliTaq Gold® DNA polymerase was purchased from Applied Biosystems (Foster City, CA).

    Polymerase Chain Reaction:

    Article Title: Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples
    Article Snippet: .. Further optimization experiments and reduction in reaction volume combined with a previous filtration protocol ( ) resulted in the final reaction conditions of 5 µl of total reaction volume consisting of 1× PCR buffer (Qiagen), 10 nM fluorecene (Bio-Rad), 3.5 mM total MgCl2 (Qiagen), 150 nM of each V6 primer (IDT), 50 nM temperature calibrator sequences (IDT), 1× EvaGreen (Sigma), 200 µM dNTP (Invitrogen), 0.05 U/µl AmpliTaq Gold Low DNA Taq polymerase (Invitrogen), 2 µl of genomic DNA (gDNA) dilution and ultrapure water (Quality Biological), with a 20 µl of overlay of high-quality DNase-free mineral oil (Sigma). .. For each bacterial gDNA template , standard 10-fold dilutions of template gDNA in ultrapure DNase-treated water ranging from approximately 106 to 0 copies per PCR reaction were run using MyIQ qPCR (Bio-Rad) machine.

    Article Title: MethyLight and Digital MethyLight
    Article Snippet: .. MethyLight PCR AmpliTaq Gold DNA Polymerase with Buffer II and MgCl2 (Thermo Fisher Scientific). ..

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    Gel Extraction:

    Article Title: Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification
    Article Snippet: .. Reagents and Instruments Oligonucleotides were purchased from Biomers or Metabion, HeLa genomic DNA and Taq 2x master mix was bought from New England Biolabs, dNTPs were either from Roche or Fermentas, Phusion DNA polymerase was purchased from Thermo Scientific, Platinum Taq and AmpliTaq Gold DNA polymerases from Life Technologies, DNase I, SphI, and HindIII from Fermentas, the Gel Extraction and EpiTect MSP kit from Qiagen and used according to their manuals. .. KlenTaq and its respective mutants were recombinantly expressed in E. coli BL21 (DE3) and purified with Ni-IDA as previously described .

    Recombinant:

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

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  • 99
    Thermo Fisher amplitaq gold360
    Amplitaq Gold360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold360/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold360 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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