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GE Healthcare amplitaq gold dna polymerase
Amplitaq Gold Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplitaq gold dna polymerase/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
amplitaq gold dna polymerase - by Bioz Stars, 2020-09
99/100 stars

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Polymerase Chain Reaction:

Article Title: Enhanced NADH Metabolism Involves Colistin-Induced Killing of Bacillus subtilis and Paenibacillus polymyxa
Article Snippet: .. PCR amplification reactions were performed in a final volume of 50 µL containing 20 ng genomic DNA, 100 nM each of primers, 62.5 µM each of dNTPs, 50 mM KCl, 10 mM Tris-HCl, 1.5 mM MgCl2 and 1 U Taq polymerase (Amersham Biosciences, Piscataway, BJ, USA). .. PCR amplification consisted of denaturation at 95 °C for 5 min, followed by 35 cycles of 30 s at 95 °C, 30 s at 55 °C, 2 min at 72 °C, and a final extension step at 72 °C for 10 min. At the end of the reaction, the reaction mixture was cooled to 4 °C to await further manipulations.

Article Title: A Recombination Hotspot in a Schizophrenia-Associated Region of GABRB2
Article Snippet: .. Amplification of a 760-bp DNA fragment that included SNPs S1 to S5 was performed in a 20 µl PCR mixture containing 100 ng sperm or genomic DNA, 75 nM of each of primers B2I8-214F and B2I8-214R , 50 nM of each dNTP, 2.5 mM MgCl2 and 1 U Taq DNA polymerase (Amersham). .. Stringent PCR conditions using increased annealing temperatures and a minimum number of reaction cycles were employed to prevent PCR jumping.

Article Title: dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
Article Snippet: .. For this purpose, Taq polymerase-dependent PCR amplification in the presence of minor amounts (5%) of dUTPs labelled with differently charged Cy3 and Cy5 analogues was investigated. dUTPs labelled with electroneutral Cy3 and Cy5 analogues were used by Taq polymerase approximately one order of magnitude more effectively than dUTPs labelled with negatively charged Cy3 or Cy5 fluorophore analogues, including Amersham Cy3-dUTP and Cy5-dUTP, each of which carries negatively charged fluorophores. ..

Activity Assay:

Article Title: dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
Article Snippet: .. This activity was not observed for Taq polymerase when dUTPs labelled with negatively charged Cy3 and Cy5 dye analogues, dU(Cy3a−)TP, dU(Cy5a−)TP and Amersham Cy5-dUTP, were used (Figures and and ). .. Nucleotidyl transferase activity of Taq polymerase in the presence of fluorescently labelled dUTPs: mass spectral analysis of primer extension products Figure illustrates the results of a mass spectral analysis of P1-(Cy3a−) primer extension products obtained using the M5 template and natural dNTPs or dNTPs in which dTTP was completely replaced by dU(Cy5a1±)TP (mass spectra 1 and 2, respectively).

Article Title: Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Article Snippet: .. We have synthesized a 582,970-base pair Mycoplasma genitalium genome. .. We have synthesized a 582,970-base pair Mycoplasma genitalium genome.

Article Title: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases
Article Snippet: .. Taq DNA polymerase (Amersham Pharmacia Biotech, Piscataway, NJ) and its F667Y derivative Thermo Sequenase (Amersham Pharmacia Biotech) naturally lack this activity, while the archaeon DNA polymerases have been genetically modified within the conserved exonuclease motif DIE to AIA to eliminate this activity ( – ). .. Vent and Deep Vent DNA polymerases were from New England Biolabs and Pfu DNA polymerase was from Stratagene (La Jolla, CA).

Amplification:

Article Title: Enhanced NADH Metabolism Involves Colistin-Induced Killing of Bacillus subtilis and Paenibacillus polymyxa
Article Snippet: .. PCR amplification reactions were performed in a final volume of 50 µL containing 20 ng genomic DNA, 100 nM each of primers, 62.5 µM each of dNTPs, 50 mM KCl, 10 mM Tris-HCl, 1.5 mM MgCl2 and 1 U Taq polymerase (Amersham Biosciences, Piscataway, BJ, USA). .. PCR amplification consisted of denaturation at 95 °C for 5 min, followed by 35 cycles of 30 s at 95 °C, 30 s at 55 °C, 2 min at 72 °C, and a final extension step at 72 °C for 10 min. At the end of the reaction, the reaction mixture was cooled to 4 °C to await further manipulations.

Article Title: A Recombination Hotspot in a Schizophrenia-Associated Region of GABRB2
Article Snippet: .. Amplification of a 760-bp DNA fragment that included SNPs S1 to S5 was performed in a 20 µl PCR mixture containing 100 ng sperm or genomic DNA, 75 nM of each of primers B2I8-214F and B2I8-214R , 50 nM of each dNTP, 2.5 mM MgCl2 and 1 U Taq DNA polymerase (Amersham). .. Stringent PCR conditions using increased annealing temperatures and a minimum number of reaction cycles were employed to prevent PCR jumping.

Article Title: dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
Article Snippet: .. For this purpose, Taq polymerase-dependent PCR amplification in the presence of minor amounts (5%) of dUTPs labelled with differently charged Cy3 and Cy5 analogues was investigated. dUTPs labelled with electroneutral Cy3 and Cy5 analogues were used by Taq polymerase approximately one order of magnitude more effectively than dUTPs labelled with negatively charged Cy3 or Cy5 fluorophore analogues, including Amersham Cy3-dUTP and Cy5-dUTP, each of which carries negatively charged fluorophores. ..

Genetically Modified:

Article Title: Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases
Article Snippet: .. Taq DNA polymerase (Amersham Pharmacia Biotech, Piscataway, NJ) and its F667Y derivative Thermo Sequenase (Amersham Pharmacia Biotech) naturally lack this activity, while the archaeon DNA polymerases have been genetically modified within the conserved exonuclease motif DIE to AIA to eliminate this activity ( – ). .. Vent and Deep Vent DNA polymerases were from New England Biolabs and Pfu DNA polymerase was from Stratagene (La Jolla, CA).

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    GE Healthcare amplitaq gold dna polymerase
    Amplitaq Gold Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
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