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Bio-Rad amplitaq gold dna polymerase
Amplitaq Gold Dna Polymerase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplitaq gold dna polymerase/product/Bio-Rad
Average 90 stars, based on 6 article reviews
Price from $9.99 to $1999.99
amplitaq gold dna polymerase - by Bioz Stars, 2020-09
90/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Shedding of Ovine Herpesvirus 2 in Sheep Nasal Secretions: the Predominant Mode for Transmission
Article Snippet: .. The thermal cycle profile used was as follows: 95°C for 10 min to denature DNA and activate the AmpliTaq Gold DNA polymerase, followed by 40 cycles at 60°C for 1 min and at 95°C for 15 s. Amplifications were performed in an iCycler iQ real-time PCR detection system (Bio-Rad Laboratories, Hercules, Calif.), and data were analyzed using software (version 2.3) provided by the manufacturer. ..

Article Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues
Article Snippet: .. The TaqMan™ Universal PCR Master Mix (used in the qPCR Harper’s test) contains an AmpliTaq Gold DNA polymerase, and the SsoAdvanced™ Universal Probes Supermix (Bio-Rad) (used in our tetraplex qPCR assays) contains a Sso7d fusion polymerase. .. Both Taq polymerases were highlighted to have good amplification performance in comparison to nine other Taq polymerases ( ).

Article Title: Pig BMSCs Transfected with Human TFPI Combat Species Incompatibility and Regulate the Human TF Pathway in Vitro and in a Rodent Model.
Article Snippet: .. AmpliTaq Gold DNA polymerase was activated for 10 minutes at 95 °C, and amplification was performed for 40 cycles of 95 °C for 15 seconds and 60 °C for 1 minute on a Multicolour Real-time PCR detection system IQ 5 (Bio-Rad, USA). .. Cell Physiol Biochem 2015;36:233-249 DOI: 10.1159/000374067 Published online: May 04, 2015 © 2015 S. Karger AG, Basel www.karger.com/cpb 238 Hongchen et al.: Coagulation Dysregulation due to Xenotransplantation.

Article Title: Novel Tetraplex Quantitative PCR Assays for Simultaneous Detection and Identification of Xylella fastidiosa Subspecies in Plant Tissues
Article Snippet: .. The TaqMan™ Universal PCR Master Mix (used in the qPCR Harper's test) contains an AmpliTaq Gold DNA polymerase, and the SsoAdvanced™ Universal Probes Supermix (Bio-Rad) (used in our tetraplex qPCR assays) contains an Sso7d fusion polymerase. .. Both Taq polymerases were highlighted to have good amplification performance in comparison to nine other Taq polymerases ( ).

Article Title: Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species
Article Snippet: .. Real-time PCR testing and melting curve analysis To perform real-time qPCR assays, Power SYBR Green PCR Master Mix containing AmpliTaq gold DNA polymerase (Bio-Rad) was used. .. Real-time PCR was performed in triplicates using the CFX96 Real-Time PCR Detection System (Bio-Rad) with the following parameters: an initial activation of AmpliTaq gold DNA polymerase at 95°C for 10 minutes, followed by 50 cycles of 95°C for 30 seconds (denaturation) and 60°C for one minute (annealing and extension).

Article Title: Identification of DNA Signatures Suitable for Use in Development of Real-Time PCR Assays by Whole-Genome Sequence Approaches: Use of Streptococcus pyogenes in a Pilot Study
Article Snippet: .. Power SYBR green PCR master mix with AmpliTaq gold DNA polymerase and a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) were used with the following cycling parameters: an initial activation of AmpliTaq gold DNA polymerase at 94°C for 10 min, followed by 50 cycles of 94°C for 15 s (denaturation) and 60°C for 1 min. .. Among the 11 remaining primer sets, 2 primer sets (sets 4 and 6) ( ) produced some side amplicons from non- S. pyogenes DNA with melting peaks close to those of S. pyogenes .

Article Title: Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection
Article Snippet: .. 2 μg of RNA was converted into cDNA with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, product ID 4368814) according to the manufacturer’s manual and concentrations and A260/280 were measured with NanoDrop, typically yielding DNA with A260/280 of 1.8. qPCR reactions were prepared with SYBR Green PCR Master Mix (Thermo Fisher Scientific, product ID 4309155), which includes AmpliTaq Gold DNA Polymerase, according to the manufacturer’s manual with 2 μM each of forward and reverse primers and performed on a Bio-Rad CFX96 machine. ..

Amplification:

Article Title: Pig BMSCs Transfected with Human TFPI Combat Species Incompatibility and Regulate the Human TF Pathway in Vitro and in a Rodent Model.
Article Snippet: .. AmpliTaq Gold DNA polymerase was activated for 10 minutes at 95 °C, and amplification was performed for 40 cycles of 95 °C for 15 seconds and 60 °C for 1 minute on a Multicolour Real-time PCR detection system IQ 5 (Bio-Rad, USA). .. Cell Physiol Biochem 2015;36:233-249 DOI: 10.1159/000374067 Published online: May 04, 2015 © 2015 S. Karger AG, Basel www.karger.com/cpb 238 Hongchen et al.: Coagulation Dysregulation due to Xenotransplantation.

SYBR Green Assay:

Article Title: Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species
Article Snippet: .. Real-time PCR testing and melting curve analysis To perform real-time qPCR assays, Power SYBR Green PCR Master Mix containing AmpliTaq gold DNA polymerase (Bio-Rad) was used. .. Real-time PCR was performed in triplicates using the CFX96 Real-Time PCR Detection System (Bio-Rad) with the following parameters: an initial activation of AmpliTaq gold DNA polymerase at 95°C for 10 minutes, followed by 50 cycles of 95°C for 30 seconds (denaturation) and 60°C for one minute (annealing and extension).

Article Title: Identification of DNA Signatures Suitable for Use in Development of Real-Time PCR Assays by Whole-Genome Sequence Approaches: Use of Streptococcus pyogenes in a Pilot Study
Article Snippet: .. Power SYBR green PCR master mix with AmpliTaq gold DNA polymerase and a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) were used with the following cycling parameters: an initial activation of AmpliTaq gold DNA polymerase at 94°C for 10 min, followed by 50 cycles of 94°C for 15 s (denaturation) and 60°C for 1 min. .. Among the 11 remaining primer sets, 2 primer sets (sets 4 and 6) ( ) produced some side amplicons from non- S. pyogenes DNA with melting peaks close to those of S. pyogenes .

Article Title: Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection
Article Snippet: .. 2 μg of RNA was converted into cDNA with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, product ID 4368814) according to the manufacturer’s manual and concentrations and A260/280 were measured with NanoDrop, typically yielding DNA with A260/280 of 1.8. qPCR reactions were prepared with SYBR Green PCR Master Mix (Thermo Fisher Scientific, product ID 4309155), which includes AmpliTaq Gold DNA Polymerase, according to the manufacturer’s manual with 2 μM each of forward and reverse primers and performed on a Bio-Rad CFX96 machine. ..

Activation Assay:

Article Title: Identification of DNA Signatures Suitable for Use in Development of Real-Time PCR Assays by Whole-Genome Sequence Approaches: Use of Streptococcus pyogenes in a Pilot Study
Article Snippet: .. Power SYBR green PCR master mix with AmpliTaq gold DNA polymerase and a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) were used with the following cycling parameters: an initial activation of AmpliTaq gold DNA polymerase at 94°C for 10 min, followed by 50 cycles of 94°C for 15 s (denaturation) and 60°C for 1 min. .. Among the 11 remaining primer sets, 2 primer sets (sets 4 and 6) ( ) produced some side amplicons from non- S. pyogenes DNA with melting peaks close to those of S. pyogenes .

Polymerase Chain Reaction:

Article Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues
Article Snippet: .. The TaqMan™ Universal PCR Master Mix (used in the qPCR Harper’s test) contains an AmpliTaq Gold DNA polymerase, and the SsoAdvanced™ Universal Probes Supermix (Bio-Rad) (used in our tetraplex qPCR assays) contains a Sso7d fusion polymerase. .. Both Taq polymerases were highlighted to have good amplification performance in comparison to nine other Taq polymerases ( ).

Article Title: Novel Tetraplex Quantitative PCR Assays for Simultaneous Detection and Identification of Xylella fastidiosa Subspecies in Plant Tissues
Article Snippet: .. The TaqMan™ Universal PCR Master Mix (used in the qPCR Harper's test) contains an AmpliTaq Gold DNA polymerase, and the SsoAdvanced™ Universal Probes Supermix (Bio-Rad) (used in our tetraplex qPCR assays) contains an Sso7d fusion polymerase. .. Both Taq polymerases were highlighted to have good amplification performance in comparison to nine other Taq polymerases ( ).

Article Title: Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species
Article Snippet: .. Real-time PCR testing and melting curve analysis To perform real-time qPCR assays, Power SYBR Green PCR Master Mix containing AmpliTaq gold DNA polymerase (Bio-Rad) was used. .. Real-time PCR was performed in triplicates using the CFX96 Real-Time PCR Detection System (Bio-Rad) with the following parameters: an initial activation of AmpliTaq gold DNA polymerase at 95°C for 10 minutes, followed by 50 cycles of 95°C for 30 seconds (denaturation) and 60°C for one minute (annealing and extension).

Article Title: Identification of DNA Signatures Suitable for Use in Development of Real-Time PCR Assays by Whole-Genome Sequence Approaches: Use of Streptococcus pyogenes in a Pilot Study
Article Snippet: .. Power SYBR green PCR master mix with AmpliTaq gold DNA polymerase and a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) were used with the following cycling parameters: an initial activation of AmpliTaq gold DNA polymerase at 94°C for 10 min, followed by 50 cycles of 94°C for 15 s (denaturation) and 60°C for 1 min. .. Among the 11 remaining primer sets, 2 primer sets (sets 4 and 6) ( ) produced some side amplicons from non- S. pyogenes DNA with melting peaks close to those of S. pyogenes .

Article Title: Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection
Article Snippet: .. 2 μg of RNA was converted into cDNA with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, product ID 4368814) according to the manufacturer’s manual and concentrations and A260/280 were measured with NanoDrop, typically yielding DNA with A260/280 of 1.8. qPCR reactions were prepared with SYBR Green PCR Master Mix (Thermo Fisher Scientific, product ID 4309155), which includes AmpliTaq Gold DNA Polymerase, according to the manufacturer’s manual with 2 μM each of forward and reverse primers and performed on a Bio-Rad CFX96 machine. ..

Software:

Article Title: Shedding of Ovine Herpesvirus 2 in Sheep Nasal Secretions: the Predominant Mode for Transmission
Article Snippet: .. The thermal cycle profile used was as follows: 95°C for 10 min to denature DNA and activate the AmpliTaq Gold DNA polymerase, followed by 40 cycles at 60°C for 1 min and at 95°C for 15 s. Amplifications were performed in an iCycler iQ real-time PCR detection system (Bio-Rad Laboratories, Hercules, Calif.), and data were analyzed using software (version 2.3) provided by the manufacturer. ..

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  • 90
    Bio-Rad amplitaq gold dna polymerase
    Amplitaq Gold Dna Polymerase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Bio-Rad
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    85
    Bio-Rad abi amplitaq gold dna polymerase system
    Abi Amplitaq Gold Dna Polymerase System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi amplitaq gold dna polymerase system/product/Bio-Rad
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    abi amplitaq gold dna polymerase system - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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