amplitaq gold 360 dna polymerase  (Thermo Fisher)


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    Name:
    AmpliTaq Gold 360 DNA Polymerase
    Description:
    The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase when used with improved AmpliTaq Gold 360 Buffer and the optional 360 GC Enhancer amplifies a vast range of DNA sequence contexts AmpliTaq Gold 360 DNA Polymerase delivers 360° coverage for a full range of targets Optimized for the broadest range of targets from everyday to challenging Unmatched sensitivity specificity and yield Robust amplification of GC rich sequences with market leading 360 GC Enhancer Achieves the highest quality sequencing dataOptimized for Easy and Challenging TargetsChallenging targets include AT rich GC rich primer dimer forming amplicons homopolymer repeats and amplicons that pose sequencing challenges Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions Figure 1 Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as the best performing enzyme ensuring the highest probability of success for the amplification of both everyday and challenging targets Table 1 As shown in Figure 1 GC rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold while AmpliTaq Gold 360 provides successful robust amplification Optimized for Hot Start PCRAmpliTaq Gold 360 DNA Polymerase provides the same hot start specificity as AmpliTaq Gold DNA polymerase A high temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed When the polymerase is added to the reaction mixture at room temperature primer extension does not occur because the enzyme is inactivated Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified Hence PCR setup can be performed at room temperature without concern for extension at misprimed sites The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number and specific product yield increases without buildup of nonspecific products including primer dimers Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2 The exquisite specificity allows easier multiplexing and allelic discrimination The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number and specific product yield increases without buildup of nonspecific products including primer dimers Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A B The exquisite specificity allows easier multiplexing and allelic discrimination Superior Sensitivity and Amplification LengthCompared to the original AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates including those from bacterial and human genomes AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number Figure 3 even in the presence of high concentrations of complex DNA making it especially suited for low copy pathogen detection and amplification of targets from degraded DNA samples The extreme purity of the enzyme contributes to its unmatched sensitivity AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long up to 5 Kb sequences Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA Note See user s manual or package insert for limited label license and trademark information For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4398813
    Price:
    None
    Applications:
    Amplification of Bisulfite-Treated DNA|GC-Rich PCR|Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|RNAi, Epigenetics & Non-Coding RNA Research|Routine PCR|Methylation Analysis
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher amplitaq gold 360 dna polymerase
    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus <t>AmpliTaq</t> <t>Gold</t> 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase when used with improved AmpliTaq Gold 360 Buffer and the optional 360 GC Enhancer amplifies a vast range of DNA sequence contexts AmpliTaq Gold 360 DNA Polymerase delivers 360° coverage for a full range of targets Optimized for the broadest range of targets from everyday to challenging Unmatched sensitivity specificity and yield Robust amplification of GC rich sequences with market leading 360 GC Enhancer Achieves the highest quality sequencing dataOptimized for Easy and Challenging TargetsChallenging targets include AT rich GC rich primer dimer forming amplicons homopolymer repeats and amplicons that pose sequencing challenges Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions Figure 1 Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as the best performing enzyme ensuring the highest probability of success for the amplification of both everyday and challenging targets Table 1 As shown in Figure 1 GC rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold while AmpliTaq Gold 360 provides successful robust amplification Optimized for Hot Start PCRAmpliTaq Gold 360 DNA Polymerase provides the same hot start specificity as AmpliTaq Gold DNA polymerase A high temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed When the polymerase is added to the reaction mixture at room temperature primer extension does not occur because the enzyme is inactivated Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified Hence PCR setup can be performed at room temperature without concern for extension at misprimed sites The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number and specific product yield increases without buildup of nonspecific products including primer dimers Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2 The exquisite specificity allows easier multiplexing and allelic discrimination The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number and specific product yield increases without buildup of nonspecific products including primer dimers Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A B The exquisite specificity allows easier multiplexing and allelic discrimination Superior Sensitivity and Amplification LengthCompared to the original AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates including those from bacterial and human genomes AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number Figure 3 even in the presence of high concentrations of complex DNA making it especially suited for low copy pathogen detection and amplification of targets from degraded DNA samples The extreme purity of the enzyme contributes to its unmatched sensitivity AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long up to 5 Kb sequences Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA Note See user s manual or package insert for limited label license and trademark information For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/amplitaq gold 360 dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 dna polymerase - by Bioz Stars, 2020-09
    99/100 stars

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    1) Product Images from "Molecular breeding of polymerases for resistance to environmental inhibitors"

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkq1360

    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    Figure Legend Snippet: Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Techniques Used: Inhibition, Polymerase Chain Reaction, Activity Assay

    Related Articles

    DNA Extraction:

    Article Title: Ancient DNA provides evidence of 27,000-year-old papillomavirus infection and long-term codivergence with rodents
    Article Snippet: .. In the first round, 2 μl of the DNA extraction was amplified using AmpliTaq Gold 360 DNA polymerase (Thermo Fisher Scientific), in a 50 μl reaction volume, with a final concentration of 0.4 μM of each primer, 200 μM dNTP, 1× AmpliTaq Gold 360 Buffer, and 2.5 μM of MgCl2 . ..

    Multiplex Assay:

    Article Title: Multilevel regression modeling for aneuploidy classification and physical separation of maternal cell contamination facilitates the QF-PCR based analysis of common fetal aneuploidies
    Article Snippet: .. The multiplex reaction was performed in the volume of 20 μl consisting of 1X AmpliTaq Gold 360 Buffer (Thermo Fisher Scientific), 1.875 mM MgCl2 , 187.5 μM each of the four dNTPs (dATP, dCTP, dGTP, and dTTP), 1.5 μl 360 GC Enhancer (Thermo Fisher Scientific), 50–667 nM primers, 0.75 U AmpliTaq Gold 360 DNA Polymerase (Thermo Fisher Scientific) and 1–50 ng DNA. .. The conditions for the PCR reaction were: 10 min at 95°C for the activation of the DNA polymerase, 29 cycles each consisted of 45 seconds at 95°C for DNA denaturation, 1 min. at 58°C for primer annealing and 1 min. 30 seconds at 72°C for elongation and finally one cycle of 30 min at 60°C for removing the stutter peaks.

    Amplification:

    Article Title: Ancient DNA provides evidence of 27,000-year-old papillomavirus infection and long-term codivergence with rodents
    Article Snippet: .. In the first round, 2 μl of the DNA extraction was amplified using AmpliTaq Gold 360 DNA polymerase (Thermo Fisher Scientific), in a 50 μl reaction volume, with a final concentration of 0.4 μM of each primer, 200 μM dNTP, 1× AmpliTaq Gold 360 Buffer, and 2.5 μM of MgCl2 . ..

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We amplified the PrP gene ( PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We purified amplicons by using an Illustra ExoProStar 1-Step clean-up kit (GE Healthcare Life Sciences, Little Chalfont, UK).

    Polymerase Chain Reaction:

    Article Title: Role of Material-Driven Fibronectin Fibrillogenesis in Protein Remodeling
    Article Snippet: .. PCR experiments were done using Ampli Taq Gold 360 DNA polymerase (Invitrogen) Oligonucleotides are shown in . .. Metalloproteinase activity analysis Gelatin zymography was performed using 15 μL of supernatant after 4 and 24 h of culture.

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We amplified the PrP gene ( PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We purified amplicons by using an Illustra ExoProStar 1-Step clean-up kit (GE Healthcare Life Sciences, Little Chalfont, UK).

    Article Title: Comparative Genomics of an IncA/C Multidrug Resistance Plasmid from Escherichia coli and Klebsiella Isolates from Intensive Care Unit Patients and the Utility of Whole-Genome Sequencing in Health Care Settings
    Article Snippet: .. The PCR was performed using AmpliTaq Gold 360 polymerase (Life Technologies). .. The PCR was performed using the following final concentrations of reagents in a 15-μl final reaction volume: 1× buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.2 μM each primer, and 1.25 U of Taq polymerase.

    Article Title: MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα
    Article Snippet: .. RT products were stored at −20°C prior to RT-qPCR. qPCR was performed using 25 µl volumes on 96-well plates with a reaction buffer containing 1xTaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Inc., Waltham, MA, USA), 3 mM Mn(OAc)2 , 200 µM dNTPs, 1×25 U AmpliTaq Gold polymerase (#4398823, Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1×25 U AmpErase UNG (#N8080096, Thermo Fisher Scientific, Inc.), 100–200 nmol TaqMan probe (#4316034, Thermo Fisher Scientific, Inc.) and 900 nmol primers (sequences presented in ). .. U6 snRNA served as an endogenous control and was used to normalize the expression levels of miR-200b.

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
    Article Snippet: .. Inhibition profiles for AmpliTaq Gold 360 DNA polymerase (Applied Biosystems) were determined using an identical PCR set-up but using AmpliTaq Gold buffer and thermocycling conditions according to manufacturer’s recommendations. .. Briefly, inhibitor suspensions were prepared as in ‘Other inhibitors: provenance and preparation’ section and added at 100% inhibitory concentration to PCR mix AT comprising 1× AmpliTaqGold buffer supplemented with 2 mM MgCl2 , dNTPs (20 µM final), primers 5′-AAA AAT CTA GAT AAC GAG GGC AA/5′-ACC ACC GAA CTG CGG GTG ACG CCA AGC G (at 0.1 µM each), 1 pM of pASK75 template.

    Quantitative RT-PCR:

    Article Title: MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα
    Article Snippet: .. RT products were stored at −20°C prior to RT-qPCR. qPCR was performed using 25 µl volumes on 96-well plates with a reaction buffer containing 1xTaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Inc., Waltham, MA, USA), 3 mM Mn(OAc)2 , 200 µM dNTPs, 1×25 U AmpliTaq Gold polymerase (#4398823, Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1×25 U AmpErase UNG (#N8080096, Thermo Fisher Scientific, Inc.), 100–200 nmol TaqMan probe (#4316034, Thermo Fisher Scientific, Inc.) and 900 nmol primers (sequences presented in ). .. U6 snRNA served as an endogenous control and was used to normalize the expression levels of miR-200b.

    Real-time Polymerase Chain Reaction:

    Article Title: An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications
    Article Snippet: .. Using the home-made qPCR mix, various Taq polymerases were tested for contamination with exogenous DNA: AmpliTaq Gold and AmpliTaq 360 DNA Polymerase (Applied Biosystems, CA, USA), FastStart Taq DNA polymerase (Roche Applied Science, Mannheim, Germany), HotStarTaq® DNA polymerase (Qiagen, Düsseldorf, Germany), GoTaq® Hot Start Polymerase (Promega Corporation, Madison, WI, USA). .. Contamination of PCR reagents with foreign DNA was tested using primers amplifying the B. taurus and the human mitochondrial D-loop.

    Article Title: MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα
    Article Snippet: .. RT products were stored at −20°C prior to RT-qPCR. qPCR was performed using 25 µl volumes on 96-well plates with a reaction buffer containing 1xTaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Inc., Waltham, MA, USA), 3 mM Mn(OAc)2 , 200 µM dNTPs, 1×25 U AmpliTaq Gold polymerase (#4398823, Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1×25 U AmpErase UNG (#N8080096, Thermo Fisher Scientific, Inc.), 100–200 nmol TaqMan probe (#4316034, Thermo Fisher Scientific, Inc.) and 900 nmol primers (sequences presented in ). .. U6 snRNA served as an endogenous control and was used to normalize the expression levels of miR-200b.

    Concentration Assay:

    Article Title: Ancient DNA provides evidence of 27,000-year-old papillomavirus infection and long-term codivergence with rodents
    Article Snippet: .. In the first round, 2 μl of the DNA extraction was amplified using AmpliTaq Gold 360 DNA polymerase (Thermo Fisher Scientific), in a 50 μl reaction volume, with a final concentration of 0.4 μM of each primer, 200 μM dNTP, 1× AmpliTaq Gold 360 Buffer, and 2.5 μM of MgCl2 . ..

    Inhibition:

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
    Article Snippet: .. Inhibition profiles for AmpliTaq Gold 360 DNA polymerase (Applied Biosystems) were determined using an identical PCR set-up but using AmpliTaq Gold buffer and thermocycling conditions according to manufacturer’s recommendations. .. Briefly, inhibitor suspensions were prepared as in ‘Other inhibitors: provenance and preparation’ section and added at 100% inhibitory concentration to PCR mix AT comprising 1× AmpliTaqGold buffer supplemented with 2 mM MgCl2 , dNTPs (20 µM final), primers 5′-AAA AAT CTA GAT AAC GAG GGC AA/5′-ACC ACC GAA CTG CGG GTG ACG CCA AGC G (at 0.1 µM each), 1 pM of pASK75 template.

    Sequencing:

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We amplified the PrP gene ( PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We purified amplicons by using an Illustra ExoProStar 1-Step clean-up kit (GE Healthcare Life Sciences, Little Chalfont, UK).

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  • 99
    Thermo Fisher amplitaq gold 360 master mix
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq <t>Gold</t> 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Gold 360 Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 master mix/product/Thermo Fisher
    Average 99 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 master mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    89
    Thermo Fisher amplitaq gold 360 dna polymerase
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq <t>Gold</t> 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Gold 360 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 dna polymerase/product/Thermo Fisher
    Average 89 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 dna polymerase - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    Techniques: Polymerase Chain Reaction, Hot Start PCR