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Roche amplification oligos
Amplification Oligos, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplification oligos/product/Roche
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
amplification oligos - by Bioz Stars, 2020-08
85/100 stars

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Sequencing:

Article Title: Direct Identification of an HPV-16 Tumor Antigen from Cervical Cancer Biopsy Specimens
Article Snippet: .. The amplification oligos were fusion primers containing these sequences as well as standard Roche/454 “A” and “B” sequencing adapters and unique multiplex identifier DNA barcodes (MIDs). .. PCR products were purified using Amplure-XP SPRI beads (Beckman Coulter Genomics, Danvers, MA, USA) and subsequently pooled to create a multiplexed sequencing library.

Polymerase Chain Reaction:

Article Title: Rapid and Specific Detection of Mycobacterium tuberculosis from Acid-Fast Bacillus Smear-Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real-Time PCR
Article Snippet: .. For each sample, 2 μl of template DNA was incorporated into a 10-μl PCR containing the amplification oligonucleotides and MTB-ITS hybridization probes using the LightCycler DNA Master Hybridization kit (Roche Biochemicals). .. The optimized LC PCR protocol included an initial denaturation step at 95°C for 30 s and was followed by a touchdown PCR protocol using the following conditions: 95°C for 0 s, 61°C for 15 s, and 72°C for 30 s for five cycles; 95°C for 0 s, 60°C for 15 s, and 72°C for 30 s for 5 cycles; 95°C for 0 s, 59°C for 15 s, and 72°C for 30 s for five cycles; and 95°C for 0 s, 59°C for 5 s, and 72°C for 30 s for 35 cycles.

Multiplex Assay:

Article Title: Direct Identification of an HPV-16 Tumor Antigen from Cervical Cancer Biopsy Specimens
Article Snippet: .. The amplification oligos were fusion primers containing these sequences as well as standard Roche/454 “A” and “B” sequencing adapters and unique multiplex identifier DNA barcodes (MIDs). .. PCR products were purified using Amplure-XP SPRI beads (Beckman Coulter Genomics, Danvers, MA, USA) and subsequently pooled to create a multiplexed sequencing library.

Amplification:

Article Title: Direct Identification of an HPV-16 Tumor Antigen from Cervical Cancer Biopsy Specimens
Article Snippet: .. The amplification oligos were fusion primers containing these sequences as well as standard Roche/454 “A” and “B” sequencing adapters and unique multiplex identifier DNA barcodes (MIDs). .. PCR products were purified using Amplure-XP SPRI beads (Beckman Coulter Genomics, Danvers, MA, USA) and subsequently pooled to create a multiplexed sequencing library.

Article Title: Rapid and Specific Detection of Mycobacterium tuberculosis from Acid-Fast Bacillus Smear-Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real-Time PCR
Article Snippet: .. For each sample, 2 μl of template DNA was incorporated into a 10-μl PCR containing the amplification oligonucleotides and MTB-ITS hybridization probes using the LightCycler DNA Master Hybridization kit (Roche Biochemicals). .. The optimized LC PCR protocol included an initial denaturation step at 95°C for 30 s and was followed by a touchdown PCR protocol using the following conditions: 95°C for 0 s, 61°C for 15 s, and 72°C for 30 s for five cycles; 95°C for 0 s, 60°C for 15 s, and 72°C for 30 s for 5 cycles; 95°C for 0 s, 59°C for 15 s, and 72°C for 30 s for five cycles; and 95°C for 0 s, 59°C for 5 s, and 72°C for 30 s for 35 cycles.

Hybridization:

Article Title: Rapid and Specific Detection of Mycobacterium tuberculosis from Acid-Fast Bacillus Smear-Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real-Time PCR
Article Snippet: .. For each sample, 2 μl of template DNA was incorporated into a 10-μl PCR containing the amplification oligonucleotides and MTB-ITS hybridization probes using the LightCycler DNA Master Hybridization kit (Roche Biochemicals). .. The optimized LC PCR protocol included an initial denaturation step at 95°C for 30 s and was followed by a touchdown PCR protocol using the following conditions: 95°C for 0 s, 61°C for 15 s, and 72°C for 30 s for five cycles; 95°C for 0 s, 60°C for 15 s, and 72°C for 30 s for 5 cycles; 95°C for 0 s, 59°C for 15 s, and 72°C for 30 s for five cycles; and 95°C for 0 s, 59°C for 5 s, and 72°C for 30 s for 35 cycles.

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    Roche amplification oligos
    Amplification Oligos, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplification oligos/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplification oligos - by Bioz Stars, 2020-08
    85/100 stars
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