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MWG-Biotech amplicons
Conservation of AtcS and AtcR among T. denticola strains. PCR <t>amplicons</t> obtained with atcS and atcR gene-specific primer sets from seven different strains are shown in panel A. Lane NT shows PCRs with no template added. Western blotting analysis of AtcS
Amplicons, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplicons/product/MWG-Biotech
Average 92 stars, based on 12 article reviews
Price from $9.99 to $1999.99
amplicons - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Analysis of a Growth-Phase-Regulated Two-Component Regulatory System in the Periodontal Pathogen Treponema denticola ▿"

Article Title: Analysis of a Growth-Phase-Regulated Two-Component Regulatory System in the Periodontal Pathogen Treponema denticola ▿

Journal:

doi: 10.1128/JB.00046-08

Conservation of AtcS and AtcR among T. denticola strains. PCR amplicons obtained with atcS and atcR gene-specific primer sets from seven different strains are shown in panel A. Lane NT shows PCRs with no template added. Western blotting analysis of AtcS
Figure Legend Snippet: Conservation of AtcS and AtcR among T. denticola strains. PCR amplicons obtained with atcS and atcR gene-specific primer sets from seven different strains are shown in panel A. Lane NT shows PCRs with no template added. Western blotting analysis of AtcS

Techniques Used: Polymerase Chain Reaction, Western Blot

RT-PCR and Northern blot analysis of the atcR/atcS locus. Panel A shows a schematic of atcR-atcS and their up- and downstream genes (TIGR annotation). The lengths of the intergenic regions are shown in parentheses. Primer binding sites and the amplicons
Figure Legend Snippet: RT-PCR and Northern blot analysis of the atcR/atcS locus. Panel A shows a schematic of atcR-atcS and their up- and downstream genes (TIGR annotation). The lengths of the intergenic regions are shown in parentheses. Primer binding sites and the amplicons

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Northern Blot, Binding Assay

2) Product Images from "Application of Denaturing High-Performance Liquid Chromatography in Microbial Ecology: Fermentor Sludge, Compost, and Soil Community Profiling ▿"

Article Title: Application of Denaturing High-Performance Liquid Chromatography in Microbial Ecology: Fermentor Sludge, Compost, and Soil Community Profiling ▿

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.01411-08

Separation of two pure culture amplicons ( E. coli (RT = 9.07 min) and B. subtilis (RT = 16.99 min and 17.61 min) in separated dHPLC runs and injected together. The PCR product of B. subtilis shows a double peak (for an explanation, see the text).
Figure Legend Snippet: Separation of two pure culture amplicons ( E. coli (RT = 9.07 min) and B. subtilis (RT = 16.99 min and 17.61 min) in separated dHPLC runs and injected together. The PCR product of B. subtilis shows a double peak (for an explanation, see the text).

Techniques Used: Injection, Polymerase Chain Reaction

3) Product Images from "Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever "

Article Title: Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever

Journal: Infection and Immunity

doi: 10.1128/IAI.00377-06

Development of type-specific fhbA primers and evidence that some isolates represent mixed populations. Recombinant plasmids carrying fhbA1 (pET32- fhbA 1) or fhbA2 (pET32- fhbA 2) inserts were used to establish the specificity of the primer sets (indicated to the right of each panel). Once established, these primers were used to amplify fhbA from various B. hermsii isolates (indicated above each lane). The resulting amplicons were analyzed by electrophoresis in 2.5% Metaphor agarose gels and visualized by ethidium bromide staining. All methods are described in the text. Molecular size markers are indicated.
Figure Legend Snippet: Development of type-specific fhbA primers and evidence that some isolates represent mixed populations. Recombinant plasmids carrying fhbA1 (pET32- fhbA 1) or fhbA2 (pET32- fhbA 2) inserts were used to establish the specificity of the primer sets (indicated to the right of each panel). Once established, these primers were used to amplify fhbA from various B. hermsii isolates (indicated above each lane). The resulting amplicons were analyzed by electrophoresis in 2.5% Metaphor agarose gels and visualized by ethidium bromide staining. All methods are described in the text. Molecular size markers are indicated.

Techniques Used: Recombinant, Electrophoresis, Staining

Related Articles

Clone Assay:

Article Title: Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever
Article Snippet: .. The resulting amplicons were analyzed by agarose gel electrophoresis in 1% GTG-agarose gels with Tris-acetate-EDTA (TAE) buffer, cloned, and sequenced on a fee-for-service basis (MWG Biotech). ..

Article Title: In Vivo Mutations of Thymidylate Synthase (Encoded by thyA) Are Responsible for Thymidine Dependency in Clinical Small-Colony Variants of Staphylococcus aureus ▿
Article Snippet: .. The resulting amplicons were cloned into the cloning vector pCR2.1 and sent to MWG Biotech AG, Martinsried, Germany, for sequencing using the M13 vector primers. .. For complementation, the pCX19 vector was used, which is a derivative of staphylococcal low-copy-number plasmid pC194 with lipase cloned into BamHI and SmaI restriction sites under the control of an xylA promoter and a chloramphenicol resistance cassette ( ).

Article Title: Application of Denaturing High-Performance Liquid Chromatography in Microbial Ecology: Fermentor Sludge, Compost, and Soil Community Profiling ▿
Article Snippet: .. Amplicons were screened for different retention times (RTs) on dHPLC, and chosen clones were sequenced using primer T7 (MWG-Biotech, Germany). .. DGGE analysis was performed as described previously ( ).

Article Title: Analysis of a Growth-Phase-Regulated Two-Component Regulatory System in the Periodontal Pathogen Treponema denticola ▿
Article Snippet: .. The resulting amplicons were cloned into the pCR2.1 TOPO vector and the inserts sequenced on a fee-for-service basis (MWG Biotech). .. Total RNA was isolated from 100-ml cultures using the RNeasy Midi kit (Qiagen).

Multiplex Assay:

Article Title: Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues
Article Snippet: .. The results of H pylori genotyping by multiplex PCR were validated previously by single PCR assays of each target sequence and subsequent sequencing of the respective amplicons, using sequence specific primers labelled with the IRD 800 fluorescence dye (MWG-Biotech). .. The sequences of the amplificates were analysed in an automated sequencer (LI-COR DNA analyser Gene Reader 4200; MWG-Biotech) and compared with the published data of the NCBI database by BLAST analysis.

Agarose Gel Electrophoresis:

Article Title: Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever
Article Snippet: .. The resulting amplicons were analyzed by agarose gel electrophoresis in 1% GTG-agarose gels with Tris-acetate-EDTA (TAE) buffer, cloned, and sequenced on a fee-for-service basis (MWG Biotech). ..

Fluorescence:

Article Title: Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues
Article Snippet: .. The results of H pylori genotyping by multiplex PCR were validated previously by single PCR assays of each target sequence and subsequent sequencing of the respective amplicons, using sequence specific primers labelled with the IRD 800 fluorescence dye (MWG-Biotech). .. The sequences of the amplificates were analysed in an automated sequencer (LI-COR DNA analyser Gene Reader 4200; MWG-Biotech) and compared with the published data of the NCBI database by BLAST analysis.

Electrophoresis:

Article Title: Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5?-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms
Article Snippet: .. Aliquots of the amplicons (5 μl) were analyzed by electrophoresis on 1% agarose gels and visualized after staining with ethidium bromide using a gel imaging system (MWG Biotech). .. An ∼1.9-kb fragment encompassing parts of the dissimilatory sulfite reductase genes dsrA and dsrB was amplified using primers DSR1-F and DSR4-R ( ).

Sequencing:

Article Title: In Vivo Mutations of Thymidylate Synthase (Encoded by thyA) Are Responsible for Thymidine Dependency in Clinical Small-Colony Variants of Staphylococcus aureus ▿
Article Snippet: .. The resulting amplicons were cloned into the cloning vector pCR2.1 and sent to MWG Biotech AG, Martinsried, Germany, for sequencing using the M13 vector primers. .. For complementation, the pCX19 vector was used, which is a derivative of staphylococcal low-copy-number plasmid pC194 with lipase cloned into BamHI and SmaI restriction sites under the control of an xylA promoter and a chloramphenicol resistance cassette ( ).

Article Title: DHRS9 Is a Stable Marker of Human Regulatory Macrophages
Article Snippet: .. PCR specificity was confirmed sequencing of amplicons (MWG Biotech). .. DHRS9 Expression Uniquely Identifies Mregs Amongst Comparator Macrophages Although the CD14−/low CD16−/low TLR2− and CD163−/low cell-surface phenotype distinguished human Mregs from a variety of differently polarised monocyte-derived macrophages, this specification relies upon the absence of marker expression in Mregs (Figure ).

Article Title: Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues
Article Snippet: .. The results of H pylori genotyping by multiplex PCR were validated previously by single PCR assays of each target sequence and subsequent sequencing of the respective amplicons, using sequence specific primers labelled with the IRD 800 fluorescence dye (MWG-Biotech). .. The sequences of the amplificates were analysed in an automated sequencer (LI-COR DNA analyser Gene Reader 4200; MWG-Biotech) and compared with the published data of the NCBI database by BLAST analysis.

Imaging:

Article Title: Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5?-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms
Article Snippet: .. Aliquots of the amplicons (5 μl) were analyzed by electrophoresis on 1% agarose gels and visualized after staining with ethidium bromide using a gel imaging system (MWG Biotech). .. An ∼1.9-kb fragment encompassing parts of the dissimilatory sulfite reductase genes dsrA and dsrB was amplified using primers DSR1-F and DSR4-R ( ).

Polymerase Chain Reaction:

Article Title: DHRS9 Is a Stable Marker of Human Regulatory Macrophages
Article Snippet: .. PCR specificity was confirmed sequencing of amplicons (MWG Biotech). .. DHRS9 Expression Uniquely Identifies Mregs Amongst Comparator Macrophages Although the CD14−/low CD16−/low TLR2− and CD163−/low cell-surface phenotype distinguished human Mregs from a variety of differently polarised monocyte-derived macrophages, this specification relies upon the absence of marker expression in Mregs (Figure ).

Article Title: Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues
Article Snippet: .. The results of H pylori genotyping by multiplex PCR were validated previously by single PCR assays of each target sequence and subsequent sequencing of the respective amplicons, using sequence specific primers labelled with the IRD 800 fluorescence dye (MWG-Biotech). .. The sequences of the amplificates were analysed in an automated sequencer (LI-COR DNA analyser Gene Reader 4200; MWG-Biotech) and compared with the published data of the NCBI database by BLAST analysis.

Staining:

Article Title: Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5?-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms
Article Snippet: .. Aliquots of the amplicons (5 μl) were analyzed by electrophoresis on 1% agarose gels and visualized after staining with ethidium bromide using a gel imaging system (MWG Biotech). .. An ∼1.9-kb fragment encompassing parts of the dissimilatory sulfite reductase genes dsrA and dsrB was amplified using primers DSR1-F and DSR4-R ( ).

Plasmid Preparation:

Article Title: In Vivo Mutations of Thymidylate Synthase (Encoded by thyA) Are Responsible for Thymidine Dependency in Clinical Small-Colony Variants of Staphylococcus aureus ▿
Article Snippet: .. The resulting amplicons were cloned into the cloning vector pCR2.1 and sent to MWG Biotech AG, Martinsried, Germany, for sequencing using the M13 vector primers. .. For complementation, the pCX19 vector was used, which is a derivative of staphylococcal low-copy-number plasmid pC194 with lipase cloned into BamHI and SmaI restriction sites under the control of an xylA promoter and a chloramphenicol resistance cassette ( ).

Article Title: Analysis of a Growth-Phase-Regulated Two-Component Regulatory System in the Periodontal Pathogen Treponema denticola ▿
Article Snippet: .. The resulting amplicons were cloned into the pCR2.1 TOPO vector and the inserts sequenced on a fee-for-service basis (MWG Biotech). .. Total RNA was isolated from 100-ml cultures using the RNeasy Midi kit (Qiagen).

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  • 92
    MWG-Biotech amplicons
    Conservation of AtcS and AtcR among T. denticola strains. PCR <t>amplicons</t> obtained with atcS and atcR gene-specific primer sets from seven different strains are shown in panel A. Lane NT shows PCRs with no template added. Western blotting analysis of AtcS
    Amplicons, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplicons/product/MWG-Biotech
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    amplicons - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    MWG-Biotech ssu rrna gene amplicons
    Typical T-RFLP electropherograms of archaeal <t>SSU</t> rDNA <t>amplicons</t> from a rice field slurry sample on day 1 after flooding. Fingerprints were generated using FAM-labeled primers Ar912r (A) and Ar109f (B). The archaeal lineages represented in different OTUs are Methanobacteriaceae ( MB ), Methanomicrobiaceae ( MM ), Methanosarcinaceae ( MS ), Methanosaetaceae ( MX ), and RC-I to RC-VI. T-RF lengths of selected peaks (in base pairs) are indicated.
    Ssu Rrna Gene Amplicons, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ssu rrna gene amplicons/product/MWG-Biotech
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ssu rrna gene amplicons - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Conservation of AtcS and AtcR among T. denticola strains. PCR amplicons obtained with atcS and atcR gene-specific primer sets from seven different strains are shown in panel A. Lane NT shows PCRs with no template added. Western blotting analysis of AtcS

    Journal:

    Article Title: Analysis of a Growth-Phase-Regulated Two-Component Regulatory System in the Periodontal Pathogen Treponema denticola ▿

    doi: 10.1128/JB.00046-08

    Figure Lengend Snippet: Conservation of AtcS and AtcR among T. denticola strains. PCR amplicons obtained with atcS and atcR gene-specific primer sets from seven different strains are shown in panel A. Lane NT shows PCRs with no template added. Western blotting analysis of AtcS

    Article Snippet: The resulting amplicons were cloned into the pCR2.1 TOPO vector and the inserts sequenced on a fee-for-service basis (MWG Biotech).

    Techniques: Polymerase Chain Reaction, Western Blot

    RT-PCR and Northern blot analysis of the atcR/atcS locus. Panel A shows a schematic of atcR-atcS and their up- and downstream genes (TIGR annotation). The lengths of the intergenic regions are shown in parentheses. Primer binding sites and the amplicons

    Journal:

    Article Title: Analysis of a Growth-Phase-Regulated Two-Component Regulatory System in the Periodontal Pathogen Treponema denticola ▿

    doi: 10.1128/JB.00046-08

    Figure Lengend Snippet: RT-PCR and Northern blot analysis of the atcR/atcS locus. Panel A shows a schematic of atcR-atcS and their up- and downstream genes (TIGR annotation). The lengths of the intergenic regions are shown in parentheses. Primer binding sites and the amplicons

    Article Snippet: The resulting amplicons were cloned into the pCR2.1 TOPO vector and the inserts sequenced on a fee-for-service basis (MWG Biotech).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Northern Blot, Binding Assay

    Separation of two pure culture amplicons ( E. coli (RT = 9.07 min) and B. subtilis (RT = 16.99 min and 17.61 min) in separated dHPLC runs and injected together. The PCR product of B. subtilis shows a double peak (for an explanation, see the text).

    Journal: Applied and Environmental Microbiology

    Article Title: Application of Denaturing High-Performance Liquid Chromatography in Microbial Ecology: Fermentor Sludge, Compost, and Soil Community Profiling ▿

    doi: 10.1128/AEM.01411-08

    Figure Lengend Snippet: Separation of two pure culture amplicons ( E. coli (RT = 9.07 min) and B. subtilis (RT = 16.99 min and 17.61 min) in separated dHPLC runs and injected together. The PCR product of B. subtilis shows a double peak (for an explanation, see the text).

    Article Snippet: Amplicons were screened for different retention times (RTs) on dHPLC, and chosen clones were sequenced using primer T7 (MWG-Biotech, Germany).

    Techniques: Injection, Polymerase Chain Reaction

    Development of type-specific fhbA primers and evidence that some isolates represent mixed populations. Recombinant plasmids carrying fhbA1 (pET32- fhbA 1) or fhbA2 (pET32- fhbA 2) inserts were used to establish the specificity of the primer sets (indicated to the right of each panel). Once established, these primers were used to amplify fhbA from various B. hermsii isolates (indicated above each lane). The resulting amplicons were analyzed by electrophoresis in 2.5% Metaphor agarose gels and visualized by ethidium bromide staining. All methods are described in the text. Molecular size markers are indicated.

    Journal: Infection and Immunity

    Article Title: Immunological and Molecular Analyses of the Borrelia hermsii Factor H and Factor H-Like Protein 1 Binding Protein, FhbA: Demonstration of Its Utility as a Diagnostic Marker and Epidemiological Tool for Tick-Borne Relapsing Fever

    doi: 10.1128/IAI.00377-06

    Figure Lengend Snippet: Development of type-specific fhbA primers and evidence that some isolates represent mixed populations. Recombinant plasmids carrying fhbA1 (pET32- fhbA 1) or fhbA2 (pET32- fhbA 2) inserts were used to establish the specificity of the primer sets (indicated to the right of each panel). Once established, these primers were used to amplify fhbA from various B. hermsii isolates (indicated above each lane). The resulting amplicons were analyzed by electrophoresis in 2.5% Metaphor agarose gels and visualized by ethidium bromide staining. All methods are described in the text. Molecular size markers are indicated.

    Article Snippet: The resulting amplicons were analyzed by agarose gel electrophoresis in 1% GTG-agarose gels with Tris-acetate-EDTA (TAE) buffer, cloned, and sequenced on a fee-for-service basis (MWG Biotech).

    Techniques: Recombinant, Electrophoresis, Staining

    Typical T-RFLP electropherograms of archaeal SSU rDNA amplicons from a rice field slurry sample on day 1 after flooding. Fingerprints were generated using FAM-labeled primers Ar912r (A) and Ar109f (B). The archaeal lineages represented in different OTUs are Methanobacteriaceae ( MB ), Methanomicrobiaceae ( MM ), Methanosarcinaceae ( MS ), Methanosaetaceae ( MX ), and RC-I to RC-VI. T-RF lengths of selected peaks (in base pairs) are indicated.

    Journal: Applied and Environmental Microbiology

    Article Title: Archaeal Population Dynamics during Sequential Reduction Processes in Rice Field Soil

    doi:

    Figure Lengend Snippet: Typical T-RFLP electropherograms of archaeal SSU rDNA amplicons from a rice field slurry sample on day 1 after flooding. Fingerprints were generated using FAM-labeled primers Ar912r (A) and Ar109f (B). The archaeal lineages represented in different OTUs are Methanobacteriaceae ( MB ), Methanomicrobiaceae ( MM ), Methanosarcinaceae ( MS ), Methanosaetaceae ( MX ), and RC-I to RC-VI. T-RF lengths of selected peaks (in base pairs) are indicated.

    Article Snippet: Aliquots of the SSU rRNA gene amplicons (5 μl) were analyzed by electrophoresis on 1% agarose gels and visualized after staining with ethidium bromide using a gel imaging system (MWG Biotech).

    Techniques: Generated, Labeling, Mass Spectrometry